BRENDA - Enzyme Database

Purification and characterisation of the NADH acceptor reductase component of xylene monooxygenase encoded by the TOL plasmid pWWO of Pseudomonas putida mt-2

Shaw, J.; Harayama, S.; Eur. J. Biochem. 209, 51-61 (1992)

Data extracted from this reference:

Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.18.1.3
42000
-
gel filtration
Pseudomonas putida
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.14.15.26
Pseudomonas putida
-
-
-
1.18.1.3
Pseudomonas putida
P21394
enzyme acts as reductase component of xylene monooxygenase, EC 1.18.1.3
-
Purification (Commentary)
EC Number
Commentary
Organism
1.18.1.3
electron-transfer component XylA
Pseudomonas putida
Subunits
EC Number
Subunits
Commentary
Organism
1.18.1.3
?
x * 40000, SDS-PAGE
Pseudomonas putida
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.14.15.26
additional information
electron-transfer component XylA, EC 1.18.1.3 contains 1 mol FAD per mol of enzyme. The electron flow is from NADH to FAD to[2Fe-2S]
Pseudomonas putida
1.18.1.3
FAD
1 mol/mol enzyme. The electron flow is from NADH to FAD to [2Fe-2S]
Pseudomonas putida
1.18.1.3
NADH
electron-transfer component XylA. The electron flow is from NADH to FAD to [2Fe-2S]
Pseudomonas putida
1.18.1.3
[2Fe-2S]-center
protein contains 2 mol/mol of non-haem iron and 2 mol/mol of acid-labile sulfide. The electron flow is from NADH to FAD to [2Fe-2S]
Pseudomonas putida
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.14.15.26
additional information
electron-transfer component XylA, EC 1.18.1.3 contains 1 mol FAD per mol of enzyme. The electron flow is from NADH to FAD to[2Fe-2S]
Pseudomonas putida
1.18.1.3
FAD
1 mol/mol enzyme. The electron flow is from NADH to FAD to [2Fe-2S]
Pseudomonas putida
1.18.1.3
NADH
electron-transfer component XylA. The electron flow is from NADH to FAD to [2Fe-2S]
Pseudomonas putida
1.18.1.3
[2Fe-2S]-center
protein contains 2 mol/mol of non-haem iron and 2 mol/mol of acid-labile sulfide. The electron flow is from NADH to FAD to [2Fe-2S]
Pseudomonas putida
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.18.1.3
42000
-
gel filtration
Pseudomonas putida
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.18.1.3
electron-transfer component XylA
Pseudomonas putida
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
1.18.1.3
?
x * 40000, SDS-PAGE
Pseudomonas putida