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Literature summary extracted from

  • Hashimoto, T.; Yamada, Y.
    Purification and characterization of hyoscyamine 6 beta-hydroxylase from root cultures of Hyoscyamus niger L. hydroxylase and epoxidase activities in the enzyme preparation (1987), Eur. J. Biochem., 164, 277-285 .
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.14.20.13 ascorbate presence of reductant required Hyoscyamus niger
1.14.20.13 dehydroascorbate
-
 Hyoscyamus niger
1.14.20.13 dithiothreitol
-
 Hyoscyamus niger
1.14.20.13 Fe2+
-
 Hyoscyamus niger

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.14.20.13 3,4-dihydroxybenzoate competitive inhibitor with respect to 2-oxoglutarate Hyoscyamus niger
1.14.20.13 Cd2+ 0.4 mM, 100% inhibition Hyoscyamus niger
1.14.20.13 Co2+ 0.4 mM, 100% inhibition Hyoscyamus niger
1.14.20.13 Cu2+ 0.4 mM, 100% inhibition Hyoscyamus niger
1.14.20.13 EDTA 0.1 mM, 67% inhibition Hyoscyamus niger
1.14.20.13 Hg2+ 0.4 mM, 96% inhibition Hyoscyamus niger
1.14.20.13 Mn2+ 0.4 mM, 95% inhibition Hyoscyamus niger
1.14.20.13 Ni2+ 0.4 mM, 96% inhibition Hyoscyamus niger
1.14.20.13 nitroblue tetrazolium
-
 Hyoscyamus niger
1.14.20.13 Pyridine 2,4-dicarboxylate competitive inhibitor with respect to 2-oxoglutarate Hyoscyamus niger
1.14.20.13 Tiron
-
 Hyoscyamus niger
1.14.20.13 Zn2+ 0.4 mM, 100% inhibition Hyoscyamus niger

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.20.13 0.035
-
 (6S)-6beta-hydroxyhyoscyamine pH 7.8, 30°C Hyoscyamus niger
1.14.20.13 0.043
-
 2-oxoglutarate pH 7.8, 30°C Hyoscyamus niger

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.14.20.13 41000
-
 gel fitlration Hyoscyamus niger

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.20.13 6,7-dehydrohyoscyamine + 2-oxoglutarate + O2 Hyoscyamus niger
-
 scopolamine + succinate + CO2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.20.13 Hyoscyamus niger P24397
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.20.13 from root culture Hyoscyamus niger

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.14.20.13 root root culture Hyoscyamus niger
-

Storage Stability

EC Number Storage Stability Organism
1.14.20.13 -20°C, stable for at least 3 months Hyoscyamus niger

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.20.13 (6S)-6beta-hydroxyhyoscyamine + 2-oxoglutarate + O2
-
 Hyoscyamus niger scopolamine + succinate + CO2 + H2O
-
 ?
1.14.20.13 3-hydroxy-3-phenylpropionyltropine + 2-oxoglutarate + O2
-
 Hyoscyamus niger 3-hydroxy-3-phenylpropionyl-6-hydroxytropine + succinate + CO2 + H2O
-
 ?
1.14.20.13 6,7-dehydrohyoscyamine + 2-oxoglutarate + O2
-
 Hyoscyamus niger scopolamine + succinate + CO2
-
 ?
1.14.20.13 apoatropine + 2-oxoglutarate + O2
-
 Hyoscyamus niger 6-hydroxyapoatropine + succinate + CO2 + H2O
-
 ?
1.14.20.13 l-homatropine + 2-oxoglutarate + O2
-
 Hyoscyamus niger 6-hydroxyhomatropine + succinate + CO2 + H2O
-
 ?
1.14.20.13 additional information enzyme catalyzes both the hydroxylation of hyoscyamine, reaction of EC 1.14.11.11, and the epoxidation of 6beta-hydroxyhyoscyamine to generate scopolamine Hyoscyamus niger ?
-
 ?
1.14.20.13 norhyoscyamine + 2-oxoglutarate + O2
-
 Hyoscyamus niger 6-hydroxynorhyoscyamine + succinate + CO2 + H2O
-
 ?
1.14.20.13 phenylacetyltropine + 2-oxoglutarate + O2
-
 Hyoscyamus niger 6-hydroxyphenylacetyltropine + succinate + CO2 + H2O
-
 ?
1.14.20.13 t-cinnamoyltropine + 2-oxoglutarate + O2
-
 Hyoscyamus niger t-cinnamoyl-6-hydroxytropine + succinate + CO2 + H2O
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.14.20.13 7.8
-
-
 Hyoscyamus niger

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.14.20.13 0.009
-
 Pyridine 2,4-dicarboxylate pH 7.8, 30°C Hyoscyamus niger
1.14.20.13 0.09
-
 3,4-dihydroxybenzoate pH 7.8, 30°C Hyoscyamus niger