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Literature summary extracted from

  • Jones, C.P.; Ingram-Smith, C.
    Biochemical and kinetic characterization of the recombinant ADP-forming acetyl coenzyme A synthetase from the amitochondriate protozoan Entamoeba histolytica (2014), Eukaryot. Cell, 13, 1530-1537 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.2.1.B11 recombinant expression of C-terminally His6-tagged enzyme in Escherichia coli strain Rosetta2(DE3)pLysS Entamoeba histolytica
6.2.1.13 expressed in Escherichia coli Rosetta2(DE3)pLysS cells Entamoeba histolytica

Inhibitors

EC Number Inhibitors Comment Organism Structure
6.2.1.B11 acetyl phosphate
-
 Entamoeba histolytica
6.2.1.B11 AMP slight inhibition Entamoeba histolytica
6.2.1.B11 ATP ATP inhibits the acetate-forming direction of the reaction, with 50% inhibitory concentration of 0.81 mM, which is in the range of its physiological concentration. Diphosphate displays mixed inhibition versus each of the three substrates, acetyl-CoA, ADP, and phosphate. No inhibition by lactate and pyruvate Entamoeba histolytica
6.2.1.B11 cAMP
-
 Entamoeba histolytica
6.2.1.B11 D-fructose 1,6-bisphosphate
-
 Entamoeba histolytica
6.2.1.B11 D-fructose 6-phosphate
-
 Entamoeba histolytica
6.2.1.B11 D-glucose 6-phosphate
-
 Entamoeba histolytica
6.2.1.B11 diphosphate diphosphate inhibits the acetate-forming direction of the reaction, with 50% inhibitory concentration of 0.75 mM, which is in the range of its physiological concentration. ATP displays mixed inhibition versus each of the three substrates, acetyl-CoA, ADP, and phosphate Entamoeba histolytica
6.2.1.B11 glyoxylate inhibits the activity in the acetate-forming direction Entamoeba histolytica
6.2.1.B11 NAD+
-
 Entamoeba histolytica
6.2.1.B11 NADH
-
 Entamoeba histolytica
6.2.1.13 ATP inhibits the acetate-forming direction of the reaction Entamoeba histolytica
6.2.1.13 Ca2+ about 3% residual activity at 20 mM Entamoeba histolytica
6.2.1.13 Cu2+ about 15% residual activity at 20 mM Entamoeba histolytica
6.2.1.13 D-glucose 6-phosphate
-
 Entamoeba histolytica
6.2.1.13 diphosphate inhibits the acetate-forming direction of the reaction (64% residual activity at 10 mM) Entamoeba histolytica
6.2.1.13 glyoxylate 32% residual activity at 10 mM Entamoeba histolytica
6.2.1.13 NAD+ 47% residual activity at 10 mM Entamoeba histolytica
6.2.1.13 Ni2+ about 3% residual activity at 20 mM Entamoeba histolytica
6.2.1.13 Zn2+ about 5% residual activity at 20 mM Entamoeba histolytica

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.2.1.B11 additional information
-
 additional information Michaelis-Menten steady-state kinetic analysis, pseudo-first-order reaction kinetic determinations are performed in both directions of the reaction Entamoeba histolytica
6.2.1.B11 0.032
-
 propionyl-CoA pH 7.3, 37°C, propionate-forming direction, with propionyl-CoA Entamoeba histolytica
6.2.1.B11 0.04
-
 acetyl-CoA pH 7.3, 37°C, acetate-forming direction Entamoeba histolytica
6.2.1.B11 0.2
-
 CoA pH 7.3, 37°C, acetyl-CoA-forming direction, with acetate Entamoeba histolytica
6.2.1.B11 0.71
-
 ADP pH 7.3, 37°C, propionate-forming direction, with propionyl-CoA Entamoeba histolytica
6.2.1.B11 1.5
-
 phosphate pH 7.3, 37°C, propionate-forming direction, with propionyl-CoA Entamoeba histolytica
6.2.1.B11 1.6
-
 ADP pH 7.3, 37°C, acetate-forming direction, with acetyl-CoA Entamoeba histolytica
6.2.1.B11 1.6
-
 CoA pH 7.3, 37°C, propionyl-CoA-forming direction, with propionate Entamoeba histolytica
6.2.1.B11 1.8
-
 phosphate pH 7.3, 37°C, acetate-forming direction, with acetyl-CoA Entamoeba histolytica
6.2.1.B11 1.9
-
 GDP pH 7.3, 37°C, acetate-forming direction, with acetyl-CoA Entamoeba histolytica
6.2.1.B11 7.2
-
 ATP pH 7.3, 37°C, propionyl-CoA-forming direction, with propionate Entamoeba histolytica
6.2.1.B11 10
-
 GTP pH 7.3, 37°C, acetyl-CoA-forming direction, with acetate Entamoeba histolytica
6.2.1.B11 12
-
 ATP pH 7.3, 37°C, acetyl-CoA-forming direction, with acetate Entamoeba histolytica
6.2.1.B11 14
-
 acetate pH 7.3, 37°C, acetyl-CoA-forming direction Entamoeba histolytica
6.2.1.B11 29
-
 propionate pH 7.3, 37°C, propionyl-CoA-forming direction, with propionate Entamoeba histolytica
6.2.1.13 0.032
-
 propionyl-CoA at pH 7.3 and 37°C Entamoeba histolytica
6.2.1.13 0.04
-
 acetyl-CoA at pH 7.3 and 37°C Entamoeba histolytica
6.2.1.13 0.2
-
 CoA with acetate as cosubstrate, at pH 7.3 and 37°C Entamoeba histolytica
6.2.1.13 0.71
-
 ADP with propionyl-CoA as cosubstrate, at pH 7.3 and 37°C Entamoeba histolytica
6.2.1.13 1.5
-
 phosphate with propionyl-CoA as cosubstrate, at pH 7.3 and 37°C Entamoeba histolytica
6.2.1.13 1.6
-
 ADP with acetyl-CoA as cosubstrate, at pH 7.3 and 37°C Entamoeba histolytica
6.2.1.13 1.6
-
 CoA with propionate as cosubstrate, at pH 7.3 and 37°C Entamoeba histolytica
6.2.1.13 1.8
-
 phosphate with acetyl-CoA as cosubstrate, at pH 7.3 and 37°C Entamoeba histolytica
6.2.1.13 1.9
-
 GDP with acetyl-CoA as cosubstrate, at pH 7.3 and 37°C Entamoeba histolytica
6.2.1.13 7.2
-
 ATP with propionate as cosubstrate, at pH 7.3 and 37°C Entamoeba histolytica
6.2.1.13 10
-
 GTP with acetate as cosubstrate, at pH 7.3 and 37°C Entamoeba histolytica
6.2.1.13 12
-
 ATP with acetate as cosubstrate, at pH 7.3 and 37°C Entamoeba histolytica
6.2.1.13 14
-
 acetate at pH 7.3 and 37°C Entamoeba histolytica
6.2.1.13 29
-
 propionate at pH 7.3 and 37°C Entamoeba histolytica

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.2.1.B11 Ca2+ can slightly substitute for Mg2+ Entamoeba histolytica
6.2.1.B11 Co2+ can partially substitute for Mg2+ Entamoeba histolytica
6.2.1.B11 Cu2+ can slightly substitute for Mg2+ Entamoeba histolytica
6.2.1.B11 Mg2+ required for activity, best divalent cation Entamoeba histolytica
6.2.1.B11 Mn2+ can substitute for Mg2+ Entamoeba histolytica
6.2.1.B11 Ni2+ can slightly substitute for Mg2+ Entamoeba histolytica
6.2.1.B11 Zn2+ can slightly substitute for Mg2+ Entamoeba histolytica
6.2.1.13 Co2+ 90% activity in the presence of 20 mM Entamoeba histolytica
6.2.1.13 Mg2+ 100% activity in the presence of 20 mM Entamoeba histolytica
6.2.1.13 Mn2+ 100% activity in the presence of 20 mM Entamoeba histolytica

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.2.1.B11 150000
-
 recombinant His6-tagged enzyme, gel filtration Entamoeba histolytica
6.2.1.13 150000
-
 gel filtration Entamoeba histolytica

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.2.1.B11 ATP + acetate + CoA Entamoeba histolytica
-
 ADP + phosphate + acetyl-CoA
-
 r
6.2.1.B11 GTP + acetate + CoA Entamoeba histolytica
-
 GDP + phosphate + acetyl-CoA
-
 r
6.2.1.13 ADP + phosphate + acetyl-CoA Entamoeba histolytica the specific activity in the acetate-forming direction is approximately half that observed in the acetyl-CoA-forming direction ATP + acetate + CoA
-
 r
6.2.1.13 ATP + acetate + CoA Entamoeba histolytica preferred direction of reaction ADP + phosphate + acetyl-CoA
-
 r

Organism

EC Number Organism UniProt Comment Textmining
6.2.1.B11 Entamoeba histolytica Q9NAT4
-
-
6.2.1.13 Entamoeba histolytica C4LUV9
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.2.1.B11 recombinant His6-tagged enzyme from Escherichia coli strain Rosetta2(DE3)pLysS by nickel affinity chromatography Entamoeba histolytica
6.2.1.13 HisTrap HP nickel affinity column chromatography, and Superdex 200 gel filtration Entamoeba histolytica

Source Tissue

EC Number Source Tissue Comment Organism Textmining
6.2.1.B11 trophozoite
-
 Entamoeba histolytica
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.2.1.B11 ADP + phosphate + acetyl-CoA
-
 Entamoeba histolytica ATP + acetate + CoA
-
 r
6.2.1.B11 ADP + phosphate + propionyl-CoA
-
 Entamoeba histolytica ATP + propionate + CoA
-
 r
6.2.1.B11 ATP + acetate + CoA
-
 Entamoeba histolytica ADP + phosphate + acetyl-CoA
-
 r
6.2.1.B11 ATP + propionate + CoA
-
 Entamoeba histolytica ADP + phosphate + propionyl-CoA
-
 r
6.2.1.B11 GDP + phosphate + acetyl-CoA
-
 Entamoeba histolytica GTP + acetate + CoA
-
 r
6.2.1.B11 GTP + acetate + CoA
-
 Entamoeba histolytica GDP + phosphate + acetyl-CoA
-
 r
6.2.1.B11 GTP + acetate + CoA GTP gives 57% of the activity with ATP Entamoeba histolytica GDP + phosphate + acetyl-CoA
-
 r
6.2.1.B11 ITP + acetate + CoA ITP gives 15% of the activity with ATP Entamoeba histolytica IDP + phosphate + acetyl-CoA
-
 r
6.2.1.B11 additional information the enzyme can utilize both ADP/ATP and GDP/GTP in the respective directions of the reaction. Less than 4% of maximal activity is observed with isobutyrate, valerate, isovalerate, hexanoate, heptanoate, octanoate, succinate, or phenylacetate as the acyl substrate. Activity observed with ATP (representing 100% activity) is nearly double that with GTP (57%) and substantially higher than the activities observed with ITP (15%), CTP (4.1%), or UTP (1.6%), no activity is observed with TTP or diphosphate. Enzymatic activity in the acetate-forming direction is determined by measuring the release of CoA with a sulfhydryl group (CoASH) from acyl-CoA by using Ellman's thiol reagent (DTNB). Production of NTB2- by CoASH cleavage of DTNB is measured spectrophotometrically at 412 nm. Activity in the acetate-forming direction is confirmed using the hexokinase/glucose-6-phosphate dehydrogenase-coupled assay. This assay couples ATP formation to the reduction of NADP+ to NADPH, which is measured spectrophotometrically at 340 nm Entamoeba histolytica ?
-
 ?
6.2.1.13 ADP + phosphate + acetyl-CoA 100% activity Entamoeba histolytica ATP + acetate + CoA
-
 r
6.2.1.13 ADP + phosphate + acetyl-CoA the specific activity in the acetate-forming direction is approximately half that observed in the acetyl-CoA-forming direction Entamoeba histolytica ATP + acetate + CoA
-
 r
6.2.1.13 ADP + phosphate + isobutyryl-CoA less than 1% activity compared to acetyl-CoA Entamoeba histolytica ATP + isobutyrate + CoA
-
 r
6.2.1.13 ADP + phosphate + isovaleryl-CoA less than 1% activity compared to acetyl-CoA Entamoeba histolytica ATP + isovalerate + CoA
-
 r
6.2.1.13 ADP + phosphate + propionyl-CoA 16% activity compared to acetyl-CoA Entamoeba histolytica ATP + propionate + CoA
-
 r
6.2.1.13 ATP + acetate + CoA 100% activity Entamoeba histolytica ADP + phosphate + acetyl-CoA
-
 r
6.2.1.13 ATP + acetate + CoA preferred direction of reaction Entamoeba histolytica ADP + phosphate + acetyl-CoA
-
 r
6.2.1.13 ATP + butyrate + CoA 5.6% activity compared to acetate Entamoeba histolytica ADP + phosphate + butyryl-CoA
-
 r
6.2.1.13 ATP + heptanoate + CoA less than 4% activity compared to acetate Entamoeba histolytica ADP + phosphate + heptanoyl-CoA
-
 r
6.2.1.13 ATP + hexanoate + CoA less than 4% activity compared to acetate Entamoeba histolytica ADP + phosphate + hexanoyl-CoA
-
 r
6.2.1.13 ATP + isobutyrate + CoA less than 4% activity compared to acetate Entamoeba histolytica ADP + phosphate + isobutyryl-CoA
-
 r
6.2.1.13 ATP + isovalerate + CoA less than 4% activity compared to acetate Entamoeba histolytica ADP + phosphate + isovaleryl-CoA
-
 r
6.2.1.13 ATP + octanoate + CoA less than 4% activity compared to acetate Entamoeba histolytica ADP + phosphate + octanoyl-CoA
-
 r
6.2.1.13 ATP + phenylacetate + CoA less than 4% activity compared to acetate Entamoeba histolytica ADP + phosphate + phenylacetyl-CoA
-
 r
6.2.1.13 ATP + propionate + CoA 72% activity compared to acetate Entamoeba histolytica ADP + phosphate + propionyl-CoA
-
 r
6.2.1.13 ATP + succinate + CoA less than 4% activity compared to acetate Entamoeba histolytica ADP + phosphate + succinyl-CoA
-
 r
6.2.1.13 ATP + valerate + CoA less than 4% activity compared to acetate Entamoeba histolytica ADP + phosphate + valeryl-CoA
-
 r
6.2.1.13 CDP + phosphate + acetyl-CoA 7% activity compared to ADP Entamoeba histolytica CTP + acetate + CoA
-
 r
6.2.1.13 CTP + acetate + CoA 4.1% activity compared to ATP Entamoeba histolytica CDP + phosphate + acetyl-CoA
-
 r
6.2.1.13 GDP + phosphate + acetyl-CoA 106% activity compared to ADP Entamoeba histolytica GTP + acetate + CoA
-
 r
6.2.1.13 GTP + acetate + CoA 57% activity compared to ATP Entamoeba histolytica GDP + phosphate + acetyl-CoA
-
 r
6.2.1.13 IDP + phosphate + acetyl-CoA 28% activity compared to ADP Entamoeba histolytica ITP + acetate + CoA
-
 r
6.2.1.13 ITP + acetate + CoA 15% activity compared to ATP Entamoeba histolytica IDP + phosphate + acetyl-CoA
-
 r
6.2.1.13 additional information no activity with diphosphate, AMP, TDP, TTP, butyryl-CoA, succinyl-CoA, or phenylacetyl-CoA Entamoeba histolytica ?
-
 ?
6.2.1.13 UDP + phosphate + acetyl-CoA 2.8% activity compared to ADP Entamoeba histolytica UTP + acetate + CoA
-
 r
6.2.1.13 UTP + acetate + CoA 1.6% activity compared to ATP Entamoeba histolytica UDP + phosphate + acetyl-CoA
-
 r

Subunits

EC Number Subunits Comment Organism
6.2.1.B11 homodimer 2 * 78999, recombinant His6-tagged enzyme, sequence calculation and SDS-PAGE Entamoeba histolytica
6.2.1.13 homodimer 2 * 78999, calculated from amino acid sequence Entamoeba histolytica

Synonyms

EC Number Synonyms Comment Organism
6.2.1.B11 ACD
-
 Entamoeba histolytica
6.2.1.B11 Acetyl-CoA synthetase (ADP-forming)
-
 Entamoeba histolytica
6.2.1.B11 ADP-forming acetyl coenzyme A synthetase
-
 Entamoeba histolytica
6.2.1.B11 EhACD
-
 Entamoeba histolytica
6.2.1.B11 More cf. EC 6.2.1.13 Entamoeba histolytica
6.2.1.13 ACD
-
 Entamoeba histolytica
6.2.1.13 acetate:CoA ligase [ADP-forming]
-
 Entamoeba histolytica
6.2.1.13 ADP-forming acetyl-coenzyme A synthetase
-
 Entamoeba histolytica

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
6.2.1.B11 37
-
 acetate-forming assay at Entamoeba histolytica
6.2.1.B11 55
-
 acetyl-CoA-forming direction Entamoeba histolytica
6.2.1.13 55
-
-
 Entamoeba histolytica

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
6.2.1.13 37
-
 the enzyme exhibits 70% activity at 37°C Entamoeba histolytica

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
6.2.1.B11 18
-
 phosphate pH 7.3, 37°C, acetate-forming direction, with propionyl-CoA Entamoeba histolytica
6.2.1.B11 21
-
 propionyl-CoA pH 7.3, 37°C, acetate-forming direction, with propionyl-CoA Entamoeba histolytica
6.2.1.B11 24
-
 ADP pH 7.3, 37°C, acetate-forming direction, with propionyl-CoA Entamoeba histolytica
6.2.1.B11 110
-
 acetyl-CoA pH 7.3, 37°C, acetate-forming direction Entamoeba histolytica
6.2.1.B11 120
-
 phosphate pH 7.3, 37°C, acetate-forming direction, with acetyl-CoA Entamoeba histolytica
6.2.1.B11 140
-
 ADP pH 7.3, 37°C, acetate-forming direction, with acetyl-CoA Entamoeba histolytica
6.2.1.B11 170
-
 GDP pH 7.3, 37°C, acetate-forming direction, with acetyl-CoA Entamoeba histolytica
6.2.1.B11 180
-
 GTP pH 7.3, 37°C, acetyl-CoA-forming direction, with acetate Entamoeba histolytica
6.2.1.B11 190
-
 propionate pH 7.3, 37°C, propionyl-CoA-forming direction, with propionate Entamoeba histolytica
6.2.1.B11 220
-
 CoA pH 7.3, 37°C, acetyl-CoA-forming direction, with acetate Entamoeba histolytica
6.2.1.B11 240
-
 acetate pH 7.3, 37°C, acetyl-CoA-forming direction Entamoeba histolytica
6.2.1.B11 240
-
 ATP pH 7.3, 37°C, propionyl-CoA-forming direction, with propionate Entamoeba histolytica
6.2.1.B11 260
-
 CoA pH 7.3, 37°C, propionyl-CoA-forming direction, with propionate Entamoeba histolytica
6.2.1.B11 320
-
 ATP pH 7.3, 37°C, acetyl-CoA-forming direction, with acetate Entamoeba histolytica
6.2.1.13 18
-
 phosphate with propionyl-CoA as cosubstrate, at pH 7.3 and 37°C Entamoeba histolytica
6.2.1.13 21
-
 propionyl-CoA at pH 7.3 and 37°C Entamoeba histolytica
6.2.1.13 24
-
 ADP with propionyl-CoA as cosubstrate, at pH 7.3 and 37°C Entamoeba histolytica
6.2.1.13 110
-
 acetyl-CoA at pH 7.3 and 37°C Entamoeba histolytica
6.2.1.13 120
-
 phosphate with acetyl-CoA as cosubstrate, at pH 7.3 and 37°C Entamoeba histolytica
6.2.1.13 140
-
 ADP with acetyl-CoA as cosubstrate, at pH 7.3 and 37°C Entamoeba histolytica
6.2.1.13 170
-
 GDP with acetyl-CoA as cosubstrate, at pH 7.3 and 37°C Entamoeba histolytica
6.2.1.13 180
-
 GTP with acetate as cosubstrate, at pH 7.3 and 37°C Entamoeba histolytica
6.2.1.13 190
-
 propionate at pH 7.3 and 37°C Entamoeba histolytica
6.2.1.13 220
-
 CoA with acetate as cosubstrate, at pH 7.3 and 37°C Entamoeba histolytica
6.2.1.13 240
-
 acetate at pH 7.3 and 37°C Entamoeba histolytica
6.2.1.13 240
-
 ATP with propionate as cosubstrate, at pH 7.3 and 37°C Entamoeba histolytica
6.2.1.13 260
-
 CoA with propionate as cosubstrate, at pH 7.3 and 37°C Entamoeba histolytica
6.2.1.13 320
-
 ATP with acetate as cosubstrate, at pH 7.3 and 37°C Entamoeba histolytica

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
6.2.1.B11 7.3
-
 assay at Entamoeba histolytica

Cofactor

EC Number Cofactor Comment Organism Structure
6.2.1.B11 ADP
-
 Entamoeba histolytica
6.2.1.B11 ATP
-
 Entamoeba histolytica
6.2.1.B11 GDP
-
 Entamoeba histolytica
6.2.1.B11 GTP
-
 Entamoeba histolytica
6.2.1.13 ADP
-
 Entamoeba histolytica
6.2.1.13 ATP
-
 Entamoeba histolytica
6.2.1.13 GDP
-
 Entamoeba histolytica
6.2.1.13 GTP
-
 Entamoeba histolytica

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
6.2.1.B11 0.81
-
 pH 7.3, 37°C, acetate-forming direction Entamoeba histolytica ATP
6.2.1.B11 0.83
-
 pH 7.3, 37°C, acetate-forming direction Entamoeba histolytica diphosphate
6.2.1.13 0.81
-
 at pH 7.3 and 37°C Entamoeba histolytica ATP
6.2.1.13 0.83
-
 at pH 7.3 and 37°C Entamoeba histolytica diphosphate
6.2.1.13 10
-
 at pH 7.3 and 37°C Entamoeba histolytica D-glucose 6-phosphate

General Information

EC Number General Information Comment Organism
6.2.1.B11 physiological function enzyme ACD is regulated through inhibition by ATP and diphosphate in the acetate-forming direction Entamoeba histolytica

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
6.2.1.B11 6.3
-
 propionate pH 7.3, 37°C, propionyl-CoA-forming direction, with propionate Entamoeba histolytica
6.2.1.B11 12
-
 phosphate pH 7.3, 37°C, acetate-forming direction, with propionyl-CoA Entamoeba histolytica
6.2.1.B11 16
-
 acetate pH 7.3, 37°C, acetyl-CoA-forming direction Entamoeba histolytica
6.2.1.B11 18
-
 GTP pH 7.3, 37°C, acetyl-CoA-forming direction, with acetate Entamoeba histolytica
6.2.1.B11 27
-
 ATP pH 7.3, 37°C, acetyl-CoA-forming direction, with acetate Entamoeba histolytica
6.2.1.B11 33
-
 ATP pH 7.3, 37°C, propionyl-CoA-forming direction, with propionate Entamoeba histolytica
6.2.1.B11 34
-
 ADP pH 7.3, 37°C, acetate-forming direction, with propionyl-CoA Entamoeba histolytica
6.2.1.B11 65
-
 phosphate pH 7.3, 37°C, acetate-forming direction, with acetyl-CoA Entamoeba histolytica
6.2.1.B11 89
-
 ADP pH 7.3, 37°C, acetate-forming direction, with acetyl-CoA Entamoeba histolytica
6.2.1.B11 90
-
 GDP pH 7.3, 37°C, acetate-forming direction, with acetyl-CoA Entamoeba histolytica
6.2.1.B11 160
-
 CoA pH 7.3, 37°C, propionyl-CoA-forming direction, with propionate Entamoeba histolytica
6.2.1.B11 650
-
 propionyl-CoA pH 7.3, 37°C, acetate-forming direction, with propionyl-CoA Entamoeba histolytica
6.2.1.B11 1100
-
 CoA pH 7.3, 37°C, acetyl-CoA-forming direction, with acetate Entamoeba histolytica
6.2.1.B11 2600
-
 acetyl-CoA pH 7.3, 37°C, acetate-forming direction Entamoeba histolytica
6.2.1.13 6.3
-
 propionate at pH 7.3 and 37°C Entamoeba histolytica
6.2.1.13 12
-
 phosphate with propionyl-CoA as cosubstrate, at pH 7.3 and 37°C Entamoeba histolytica
6.2.1.13 16
-
 acetate at pH 7.3 and 37°C Entamoeba histolytica
6.2.1.13 18
-
 GTP with acetate as cosubstrate, at pH 7.3 and 37°C Entamoeba histolytica
6.2.1.13 27
-
 ATP with acetate as cosubstrate, at pH 7.3 and 37°C Entamoeba histolytica
6.2.1.13 33
-
 ATP with propionate as cosubstrate, at pH 7.3 and 37°C Entamoeba histolytica
6.2.1.13 34
-
 ADP with propionyl-CoA as cosubstrate, at pH 7.3 and 37°C Entamoeba histolytica
6.2.1.13 65
-
 phosphate with acetyl-CoA as cosubstrate, at pH 7.3 and 37°C Entamoeba histolytica
6.2.1.13 89
-
 ADP with acetyl-CoA as cosubstrate, at pH 7.3 and 37°C Entamoeba histolytica
6.2.1.13 90
-
 GDP with acetyl-CoA as cosubstrate, at pH 7.3 and 37°C Entamoeba histolytica
6.2.1.13 160
-
 CoA with propionate as cosubstrate, at pH 7.3 and 37°C Entamoeba histolytica
6.2.1.13 650
-
 propionyl-CoA at pH 7.3 and 37°C Entamoeba histolytica
6.2.1.13 1100
-
 CoA with acetate as cosubstrate, at pH 7.3 and 37°C Entamoeba histolytica
6.2.1.13 2600
-
 acetyl-CoA at pH 7.3 and 37°C Entamoeba histolytica