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Literature summary extracted from

  • Nguyen, T.T.; Kim, S.B.; Kim, N.M.; Kang, C.; Chung, B.; Park, J.S.; Kim, D.
    Production of steviol from steviol glucosides using beta-glycosidase from Sulfolobus solfataricus (2016), Enzyme Microb. Technol., 93-94, 157-165 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.B26 expressed in Escherichia coli Rosetta BL21(DE3)pLyS cells Saccharolobus solfataricus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.B26 D-glucose competitive inhibitor Saccharolobus solfataricus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.B26 2.04
-
 4-nitrophenyl beta-D-glucoside at pH 6.0 and 80°C Saccharolobus solfataricus
3.2.1.B26 2.25
-
 2-nitrophenyl beta-galactoside at pH 6.0 and 80°C Saccharolobus solfataricus
3.2.1.B26 15.82
-
 lactose at pH 6.0 and 80°C Saccharolobus solfataricus
3.2.1.B26 17.21
-
 stevioside at pH 6.0 and 80°C Saccharolobus solfataricus

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.B26 Saccharolobus solfataricus P22498
-
-
3.2.1.B26 Saccharolobus solfataricus DSM 1617 P22498
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.B26 Ni-Sepharose column chromatography Saccharolobus solfataricus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.B26 2-nitrophenyl beta-galactoside + H2O
-
 Saccharolobus solfataricus 2-nitrophenol + D-galactose
-
 ?
3.2.1.B26 2-nitrophenyl beta-galactoside + H2O
-
 Saccharolobus solfataricus DSM 1617 2-nitrophenol + D-galactose
-
 ?
3.2.1.B26 4-nitrophenyl beta-D-glucoside + H2O highest activity Saccharolobus solfataricus 4-nitrophenol + D-glucose
-
 ?
3.2.1.B26 4-nitrophenyl beta-D-glucoside + H2O highest activity Saccharolobus solfataricus DSM 1617 4-nitrophenol + D-glucose
-
 ?
3.2.1.B26 lactose + H2O
-
 Saccharolobus solfataricus D-glucose + D-galactose
-
 ?
3.2.1.B26 lactose + H2O
-
 Saccharolobus solfataricus DSM 1617 D-glucose + D-galactose
-
 ?
3.2.1.B26 rebaudioside A + H2O negligible activity Saccharolobus solfataricus steviol + D-glucose
-
 ?
3.2.1.B26 rebaudioside A + H2O negligible activity Saccharolobus solfataricus DSM 1617 steviol + D-glucose
-
 ?
3.2.1.B26 rubusoside + H2O 44.2% yield Saccharolobus solfataricus steviol + D-glucose
-
 ?
3.2.1.B26 rubusoside + H2O 44.2% yield Saccharolobus solfataricus DSM 1617 steviol + D-glucose
-
 ?
3.2.1.B26 stevioside + H2O
-
 Saccharolobus solfataricus steviol + D-glucose
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.B26 ? x * 61000, SDS-PAGE Saccharolobus solfataricus

Synonyms

EC Number Synonyms Comment Organism
3.2.1.B26 beta-glycosidase
-
 Saccharolobus solfataricus
3.2.1.B26 bgly
-
 Saccharolobus solfataricus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.B26 1.62
-
 stevioside at pH 6.0 and 80°C Saccharolobus solfataricus
3.2.1.B26 8.37
-
 lactose at pH 6.0 and 80°C Saccharolobus solfataricus
3.2.1.B26 24
-
 2-nitrophenyl beta-galactoside at pH 6.0 and 80°C Saccharolobus solfataricus
3.2.1.B26 38.65
-
 4-nitrophenyl beta-D-glucoside at pH 6.0 and 80°C Saccharolobus solfataricus

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.2.1.B26 0.094
-
 stevioside at pH 6.0 and 80°C Saccharolobus solfataricus
3.2.1.B26 0.53
-
 lactose at pH 6.0 and 80°C Saccharolobus solfataricus
3.2.1.B26 10.67
-
 2-nitrophenyl beta-galactoside at pH 6.0 and 80°C Saccharolobus solfataricus
3.2.1.B26 18.95
-
 4-nitrophenyl beta-D-glucoside at pH 6.0 and 80°C Saccharolobus solfataricus