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Literature summary extracted from

  • Liu, Y.; Gong, X.; Wang, C.; Du, G.; Chen, J.; Kang, Z.
    Production of glucaric acid from myo-inositol in engineered Pichia pastoris (2016), Enzyme Microb. Technol., 91, 8-16 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.13.99.1 gene miox, recombinant expression in Pichia pastoris, coexpression with urinate dehydrogenase (Udh) from Pseudomonas putida KT2440 as fusion protein Komagataella phaffii
1.13.99.1 gene mMIOX, recombinant expression in Pichia pastoris, coexpression with urinate dehydrogenase (Udh) from Pseudomonas putida KT2440, recombinant coexpression of the enzyme in Escherichia coli with myo-inositol-1-phosphate synthase from Saccharomyces cerevisiae and urinate dehydrogenase (Udh) from Pseudomonas putida KT2440 Mus musculus

Protein Variants

EC Number Protein Variants Comment Organism
1.13.99.1 additional information coexpression of the Mus musculus mMIOX and the urinate dehydrogenase (Udh) from Pseudomonas putida KT2440 as fusion protein leads highly increased glutaric acid levels from myo-inositol in transfected Pichia pastoris cells. Production of glucaric acid with a fed-batch fermentation strategy, overview. Recombinant coexpression of the enzyme in Escherichia coli strains BL21(DE3) or MG1655 with myo-inositol-1-phosphate synthase from Saccharomyces cerevisiae and urinate dehydrogenase (Udh) from Pseudomonas putida KT2440 leads to highly increased glutaric acid production, especially with addition of myo-inositol instead of glucose, production parameters, overview Mus musculus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.13.99.1 myo-inositol + O2 Mus musculus
-
 D-glucuronate + H2O
-
 ?
1.13.99.1 myo-inositol + O2 Komagataella phaffii
-
 D-glucuronate + H2O
-
 ?
1.13.99.1 myo-inositol + O2 Komagataella phaffii GS115
-
 D-glucuronate + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.13.99.1 Komagataella phaffii C4QZH8 i.e. Pichia pastoris
-
1.13.99.1 Komagataella phaffii GS115 C4QZH8 i.e. Pichia pastoris
-
1.13.99.1 Mus musculus Q9QXN5
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.13.99.1 myo-inositol + O2
-
 Mus musculus D-glucuronate + H2O
-
 ?
1.13.99.1 myo-inositol + O2
-
 Komagataella phaffii D-glucuronate + H2O
-
 ?
1.13.99.1 myo-inositol + O2
-
 Komagataella phaffii GS115 D-glucuronate + H2O
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.13.99.1 MIOX
-
 Mus musculus
1.13.99.1 MIOX
-
 Komagataella phaffii
1.13.99.1 mMIOX
-
 Mus musculus
1.13.99.1 Myo-inositol oxygenase
-
 Mus musculus
1.13.99.1 Myo-inositol oxygenase
-
 Komagataella phaffii
1.13.99.1 ppMIOX
-
 Komagataella phaffii

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.13.99.1 37
-
 assay at Mus musculus
1.13.99.1 37
-
 assay at Komagataella phaffii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.13.99.1 8
-
 assay at Mus musculus
1.13.99.1 8
-
 assay at Komagataella phaffii

Expression

EC Number Organism Comment Expression
1.13.99.1 Komagataella phaffii coexpression of the Pichia pastoris ppMIOX and the urinate dehydrogenase (Udh) from Pseudomonas putida KT2440 does not lead to accumulation of glucaric acid from myo-inositol or increased enzyme activity of neither MIOX nor Udh additional information

General Information

EC Number General Information Comment Organism
1.13.99.1 metabolism the enzyme is important in the glucaric acid synthetic pathway Mus musculus
1.13.99.1 metabolism the enzyme is important in the glucaric acid synthetic pathway Komagataella phaffii