Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Kelsall, I.; Duda, D.; Olszewski, J.; Hofmann, K.; Knebel, A.; Langevin, F.; Wood, N.; Wightman, M.; Schulman, B.; Alpi, A.
    TRIAD1 and HHARI bind to and are activated by distinct neddylated Cullin-RING ligase complexes (2013), EMBO J., 32, 2848-2860 .
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.3.2.31 additional information neddylated cullin-1-RBX1 activates auto-inhibited isoform HHARI Homo sapiens

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.2.31 expressed in Escherichia coli BL21 cells Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.2.31 [E2 ubiquitin-conjugating enzyme cullin-1]-S-ubiquitinyl-L-cysteine + [acceptor protein]-L-lysine Homo sapiens overall reaction for HHARI [E2 ubiquitin-conjugating enzyme cullin-1]-L-cysteine + [acceptor protein]-N6-ubiquitinyl-L-lysine
-
 ?
2.3.2.31 [E2 ubiquitin-conjugating enzyme cullin-2]-S-ubiquitinyl-L-cysteine + [acceptor protein]-L-lysine Homo sapiens overall reaction for HHARI [E2 ubiquitin-conjugating enzyme cullin-2]-L-cysteine + [acceptor protein]-N6-ubiquitinyl-L-lysine
-
 ?
2.3.2.31 [E2 ubiquitin-conjugating enzyme cullin-3]-S-ubiquitinyl-L-cysteine + [acceptor protein]-L-lysine Homo sapiens overall reaction for HHARI [E2 ubiquitin-conjugating enzyme cullin-3]-L-cysteine + [acceptor protein]-N6-ubiquitinyl-L-lysine
-
 ?
2.3.2.31 [E2 ubiquitin-conjugating enzyme cullin-4A]-S-ubiquitinyl-L-cysteine + [acceptor protein]-L-lysine Homo sapiens overall reaction for HHARI [E2 ubiquitin-conjugating enzyme cullin-4A]-L-cysteine + [acceptor protein]-N6-ubiquitinyl-L-lysine
-
 ?
2.3.2.31 [E2 ubiquitin-conjugating enzyme cullin-5]-S-ubiquitinyl-L-cysteine + [acceptor protein]-L-lysine Homo sapiens overall reaction for TRIAD1 [E2 ubiquitin-conjugating enzyme cullin-5]-L-cysteine + [acceptor protein]-N6-ubiquitinyl-L-lysine
-
 ?
2.3.2.31 [E2 ubiquitin-conjugating enzyme UBCH7]-S-ubiquitinyl-L-cysteine + [acceptor protein]-L-lysine Homo sapiens overall reaction for TRIAD1 [E2 ubiquitin-conjugating enzyme UBCH7]-L-cysteine + [acceptor protein]-N6-ubiquitinyl-L-lysine
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.2.31 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.2.31 GSH-Sepharose column chromatography, amylose resin column chromatography, and Ni2+-Sepharose column chromatography Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.3.2.31 HEK-293 cell
-
 Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.2.31 additional information the enzyme-substrate-interaction is neddylation-dependent Homo sapiens ?
-
 ?
2.3.2.31 [E2 ubiquitin-conjugating enzyme cullin-1]-S-ubiquitinyl-L-cysteine + [acceptor protein]-L-lysine overall reaction for HHARI Homo sapiens [E2 ubiquitin-conjugating enzyme cullin-1]-L-cysteine + [acceptor protein]-N6-ubiquitinyl-L-lysine
-
 ?
2.3.2.31 [E2 ubiquitin-conjugating enzyme cullin-2]-S-ubiquitinyl-L-cysteine + [acceptor protein]-L-lysine overall reaction for HHARI Homo sapiens [E2 ubiquitin-conjugating enzyme cullin-2]-L-cysteine + [acceptor protein]-N6-ubiquitinyl-L-lysine
-
 ?
2.3.2.31 [E2 ubiquitin-conjugating enzyme cullin-3]-S-ubiquitinyl-L-cysteine + [acceptor protein]-L-lysine overall reaction for HHARI Homo sapiens [E2 ubiquitin-conjugating enzyme cullin-3]-L-cysteine + [acceptor protein]-N6-ubiquitinyl-L-lysine
-
 ?
2.3.2.31 [E2 ubiquitin-conjugating enzyme cullin-4A]-S-ubiquitinyl-L-cysteine + [acceptor protein]-L-lysine overall reaction for HHARI Homo sapiens [E2 ubiquitin-conjugating enzyme cullin-4A]-L-cysteine + [acceptor protein]-N6-ubiquitinyl-L-lysine
-
 ?
2.3.2.31 [E2 ubiquitin-conjugating enzyme cullin-5]-S-ubiquitinyl-L-cysteine + [acceptor protein]-L-lysine overall reaction for TRIAD1 Homo sapiens [E2 ubiquitin-conjugating enzyme cullin-5]-L-cysteine + [acceptor protein]-N6-ubiquitinyl-L-lysine
-
 ?
2.3.2.31 [E2 ubiquitin-conjugating enzyme UBCH7]-S-ubiquitinyl-L-cysteine + [acceptor protein]-L-lysine overall reaction for TRIAD1 Homo sapiens [E2 ubiquitin-conjugating enzyme UBCH7]-L-cysteine + [acceptor protein]-N6-ubiquitinyl-L-lysine
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.3.2.31 HHARI
-
 Homo sapiens
2.3.2.31 RBR-type E3 ligase
-
 Homo sapiens
2.3.2.31 Triad1
-
 Homo sapiens