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Literature summary extracted from

  • Spatzal, T.; Perez, K.A.; Howard, J.B.; Rees, D.C.
    Catalysis-dependent selenium incorporation and migration in the nitrogenase active site iron-molybdenum cofactor (2015), eLife, 4, e11620 .
    View publication on PubMedView publication on EuropePMC

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.18.6.1 sitting drop vapor diffusion method, using 24-28% (w/v) PEG 8000, 0.75-0.85 M NaCl, 0.1 M imidazole/malate (pH 7.5), 1% glycerol (v/v), 0.5% 2,2,2-trifluoroethanol (v/v) and 2.5 mM Na2S2O4 Azotobacter vinelandii

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.18.6.1 CO
-
 Azotobacter vinelandii
1.18.6.1 SCN-
-
 Azotobacter vinelandii
1.18.6.1 SeCN- potent, reversible inhibitor of acetylene reduction Azotobacter vinelandii

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.18.6.1 Mg2+ 5 mM MgCl2 used in assay conditions Azotobacter vinelandii

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.18.6.1 reduced ferredoxin + H+ + N2 + ATP + H2O Azotobacter vinelandii
-
 oxidized ferredoxin + H2 + NH3 + ADP + phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.18.6.1 Azotobacter vinelandii
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.18.6.1 reduced ferredoxin + H+ + N2 + ATP + H2O
-
 Azotobacter vinelandii oxidized ferredoxin + H2 + NH3 + ADP + phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
1.18.6.1 heterotetramer x-ray crystallography Azotobacter vinelandii

Synonyms

EC Number Synonyms Comment Organism
1.18.6.1 MoFe-protein
-
 Azotobacter vinelandii

Cofactor

EC Number Cofactor Comment Organism Structure
1.18.6.1 ATP
-
 Azotobacter vinelandii
1.18.6.1 iron-molybdenum cofactor [7Fe:8S:1Se:Mo:C]-R-homocitrate cluster Azotobacter vinelandii

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.18.6.1 0.41
-
 SeCN- at pH 7.5 and 30°C Azotobacter vinelandii
1.18.6.1 12.7
-
 SCN- at pH 7.5 and 30°C Azotobacter vinelandii