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Literature summary extracted from

  • Tsujimura, S.; Murata, K.
    Electrochemical oxygen reduction catalyzed by bilirubin oxidase with the aid of 2,2'-azinobis(3-ethylbenzothiazolin-6-sulfonate) on a MgO-template carbon electrode (2015), Electrochim. Acta, 180, 555-559 .
No PubMed abstract available

Protein Variants

EC Number Protein Variants Comment Organism
1.3.3.5 additional information immobilization of the enzyme on a mesoporous carbon cryogel electrode, allowing a direct electron transfer (DET) from the carbon electrode to the type I copper site of the enzyme, and analysis of the bioelectrocatalytic reactions of the enzyme in presence and absence of a mediator. The current from the dioxygenreduction reaction (ORR), catalyzed by enzyme BOD, depends on the temperature and pH of the electrolyte.The mediated ORR catalyzed by BOD on CCG electrode is also investigated using osmium-based redox polymers. The catalytic current on the CCG electrode modified with 0.2 mg/cm2 of hydrogel consisting of an enzyme, a redox polymer and a cross linker, is 1.8 mA/cm2, which is almost five times higher than that on a flat glassy carbon electrode for the same hydrogel composition and loading. The catalytic current linearly increases with the total amount of hydrogel on the porous carbon electrode while the catalytic current on the flat electrode is indifferent to the loading. Method evaluation, detailed overview Bacillus pumilus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.3.3.5 Cu2+ the enzyme is a copper oxidase Bacillus pumilus

Organism

EC Number Organism UniProt Comment Textmining
1.3.3.5 Bacillus pumilus A8FAG9
-
-
1.3.3.5 Bacillus pumilus SAFR-032 A8FAG9
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.3.5 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) + O2
-
Bacillus pumilus ? + H2O
-
?
1.3.3.5 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) + O2
-
Bacillus pumilus SAFR-032 ? + H2O
-
?

Synonyms

EC Number Synonyms Comment Organism
1.3.3.5 BOD
-
Bacillus pumilus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.3.3.5 30 60 assay at Bacillus pumilus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.3.3.5 50
-
purified enzyme immobilized on a mesoporous carbon cryogel electrode displays high stability in solution at pH 5.0 and 50°C Bacillus pumilus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.3.3.5 4 7 assay at Bacillus pumilus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.3.3.5 5
-
purified enzyme immobilized on a mesoporous carbon cryogel electrode displays high stability in solution at pH 5.0 and 50°C Bacillus pumilus