EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
1.3.3.5 | additional information | immobilization of the enzyme on a mesoporous carbon cryogel electrode, allowing a direct electron transfer (DET) from the carbon electrode to the type I copper site of the enzyme, and analysis of the bioelectrocatalytic reactions of the enzyme in presence and absence of a mediator. The current from the dioxygenreduction reaction (ORR), catalyzed by enzyme BOD, depends on the temperature and pH of the electrolyte.The mediated ORR catalyzed by BOD on CCG electrode is also investigated using osmium-based redox polymers. The catalytic current on the CCG electrode modified with 0.2 mg/cm2 of hydrogel consisting of an enzyme, a redox polymer and a cross linker, is 1.8 mA/cm2, which is almost five times higher than that on a flat glassy carbon electrode for the same hydrogel composition and loading. The catalytic current linearly increases with the total amount of hydrogel on the porous carbon electrode while the catalytic current on the flat electrode is indifferent to the loading. Method evaluation, detailed overview | Bacillus pumilus |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
1.3.3.5 | Cu2+ | the enzyme is a copper oxidase | Bacillus pumilus |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.3.3.5 | Bacillus pumilus | A8FAG9 | - |
- |
1.3.3.5 | Bacillus pumilus SAFR-032 | A8FAG9 | - |
- |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.3.3.5 | 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) + O2 | - |
Bacillus pumilus | ? + H2O | - |
? | |
1.3.3.5 | 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) + O2 | - |
Bacillus pumilus SAFR-032 | ? + H2O | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.3.3.5 | BOD | - |
Bacillus pumilus |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.3.3.5 | 30 | 60 | assay at | Bacillus pumilus |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.3.3.5 | 50 | - |
purified enzyme immobilized on a mesoporous carbon cryogel electrode displays high stability in solution at pH 5.0 and 50°C | Bacillus pumilus |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.3.3.5 | 4 | 7 | assay at | Bacillus pumilus |
EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|---|
1.3.3.5 | 5 | - |
purified enzyme immobilized on a mesoporous carbon cryogel electrode displays high stability in solution at pH 5.0 and 50°C | Bacillus pumilus |