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Literature summary extracted from
- Biochemical analysis of recombinant CYP4A11 allelic variant enzymes W126R, K276T and S353G (2016), Drug Metab. Pharmacokinet., 31, 445-450 .
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.14.14.80 | cytochrome b5 | increases the enzyme activity | Homo sapiens |
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 1.14.14.80 | gene CYP4A11, recombinant expression of His6-tagged wild-type and mutant enzymes in Escherichia coli | Homo sapiens |
Protein Variants
| EC Number | Protein Variants | Comment | Organism |
|---|---|---|---|
| 1.14.14.80 | K276T | site-directed mutagenesis, the mutant shows increased activity compared to the wild-type enzyme | Homo sapiens |
| 1.14.14.80 | S353G | site-directed mutagenesis, the mutant shows increased activity compared to the wild-type enzyme | Homo sapiens |
| 1.14.14.80 | W126R | site-directed mutagenesis, the mutant W126R variant cannot be expressed in Escherichia coli. | Homo sapiens |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.14.14.80 | additional information | - |
additional information | Michaelis-Menten kinetics, fatty acids binding constants | Homo sapiens | |
| 1.14.14.80 | 0.014 | - |
palmitic acid | pH 7.4, 37°C, recombinant mutant S353G with cytochrome B5 | Homo sapiens |  |
| 1.14.14.80 | 0.018 | - |
palmitic acid | pH 7.4, 37°C, recombinant mutant S353G | Homo sapiens | |
| 1.14.14.80 | 0.018 | - |
myristic acid | pH 7.4, 37°C, recombinant wild-type enzyme with cytochrome B5 | Homo sapiens | |
| 1.14.14.80 | 0.019 | - |
lauric acid | pH 7.4, 37°C, recombinant wild-type enzyme | Homo sapiens | |
| 1.14.14.80 | 0.021 | - |
palmitic acid | pH 7.4, 37°C, recombinant mutant K276T | Homo sapiens | |
| 1.14.14.80 | 0.021 | - |
lauric acid | pH 7.4, 37°C, recombinant mutant S353G | Homo sapiens | |
| 1.14.14.80 | 0.021 | - |
lauric acid | pH 7.4, 37°C, recombinant wild-type enzyme, with cytochrome B5 | Homo sapiens | |
| 1.14.14.80 | 0.022 | - |
palmitic acid | pH 7.4, 37°C, recombinant wild-type enzyme | Homo sapiens | |
| 1.14.14.80 | 0.022 | - |
palmitic acid | pH 7.4, 37°C, recombinant wild-type enzyme with cytochrome B5 | Homo sapiens | |
| 1.14.14.80 | 0.023 | - |
lauric acid | pH 7.4, 37°C, recombinant mutant K276T | Homo sapiens | |
| 1.14.14.80 | 0.026 | - |
myristic acid | pH 7.4, 37°C, recombinant wild-type enzyme | Homo sapiens | |
| 1.14.14.80 | 0.029 | - |
palmitic acid | pH 7.4, 37°C, recombinant mutant K276T with cytochrome B5 | Homo sapiens | |
| 1.14.14.80 | 0.03 | - |
myristic acid | pH 7.4, 37°C, recombinant mutant K276T | Homo sapiens | |
| 1.14.14.80 | 0.033 | - |
myristic acid | pH 7.4, 37°C, recombinant mutant S353G | Homo sapiens | |
| 1.14.14.80 | 0.033 | - |
lauric acid | pH 7.4, 37°C, recombinant mutant S353G with cytochrome B5 | Homo sapiens | |
| 1.14.14.80 | 0.037 | - |
myristic acid | pH 7.4, 37°C, recombinant mutant K276T with cytochrome B5 | Homo sapiens | |
| 1.14.14.80 | 0.037 | - |
lauric acid | pH 7.4, 37°C, recombinant mutant K276T with cytochrome B5 | Homo sapiens | |
| 1.14.14.80 | 0.045 | - |
myristic acid | pH 7.4, 37°C, recombinant mutant S353G with cytochrome B5 | Homo sapiens | |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.14.14.80 | Fe2+ | in heme | Homo sapiens | |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.14.14.80 | lauric acid + [reduced NADPH-hemoprotein reductase] + O2 | Homo sapiens | - |
12-hydroxylauric acid + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
| 1.14.14.80 | myristic acid + [reduced NADPH-hemoprotein reductase] + O2 | Homo sapiens | - |
14-hydroxymyristic acid + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
| 1.14.14.80 | palmitic acid + [reduced NADPH-hemoprotein reductase] + O2 | Homo sapiens | - |
16-hydroxypalmitic acid + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.14.14.80 | Homo sapiens | Q02928 | - |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.14.14.80 | recombinant His6-tagged wild-type and mutant enzymes from Escherichia coli by nickel affinity chromatography | Homo sapiens |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 1.14.14.80 | kidney | - |
Homo sapiens | - |
| 1.14.14.80 | liver | - |
Homo sapiens | - |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.14.14.80 | lauric acid + [reduced NADPH-hemoprotein reductase] + O2 | - |
Homo sapiens | 12-hydroxylauric acid + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
| 1.14.14.80 | myristic acid + [reduced NADPH-hemoprotein reductase] + O2 | - |
Homo sapiens | 14-hydroxymyristic acid + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
| 1.14.14.80 | palmitic acid + [reduced NADPH-hemoprotein reductase] + O2 | - |
Homo sapiens | 16-hydroxypalmitic acid + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 1.14.14.80 | ? | x * 58500, recombinant His-tagged wild-type enzyme, SDS-PAGE | Homo sapiens |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 1.14.14.80 | omega-hydroxylase | - |
Homo sapiens |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.14.14.80 | 37 | - |
assay at | Homo sapiens |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.14.14.80 | 0.055 | - |
palmitic acid | pH 7.4, 37°C, recombinant wild-type enzyme | Homo sapiens | |
| 1.14.14.80 | 0.068 | - |
palmitic acid | pH 7.4, 37°C, recombinant wild-type enzyme with cytochrome B5 | Homo sapiens | |
| 1.14.14.80 | 0.088 | - |
palmitic acid | pH 7.4, 37°C, recombinant mutant S353G | Homo sapiens | |
| 1.14.14.80 | 0.1 | - |
palmitic acid | pH 7.4, 37°C, recombinant mutant K276T | Homo sapiens | |
| 1.14.14.80 | 0.115 | - |
palmitic acid | pH 7.4, 37°C, recombinant mutant S353G with cytochrome B5 | Homo sapiens | |
| 1.14.14.80 | 0.117 | - |
lauric acid | pH 7.4, 37°C, recombinant wild-type enzyme | Homo sapiens | |
| 1.14.14.80 | 0.157 | - |
myristic acid | pH 7.4, 37°C, recombinant wild-type enzyme | Homo sapiens | |
| 1.14.14.80 | 0.188 | - |
palmitic acid | pH 7.4, 37°C, recombinant mutant K276T with cytochrome B5 | Homo sapiens | |
| 1.14.14.80 | 0.193 | - |
myristic acid | pH 7.4, 37°C, recombinant wild-type enzyme with cytochrome B5 | Homo sapiens | |
| 1.14.14.80 | 0.258 | - |
lauric acid | pH 7.4, 37°C, recombinant mutant K276T | Homo sapiens | |
| 1.14.14.80 | 0.258 | - |
lauric acid | pH 7.4, 37°C, recombinant wild-type enzyme, with cytochrome B5 | Homo sapiens | |
| 1.14.14.80 | 0.278 | - |
myristic acid | pH 7.4, 37°C, recombinant mutant K276T | Homo sapiens | |
| 1.14.14.80 | 0.423 | - |
myristic acid | pH 7.4, 37°C, recombinant mutant S353G | Homo sapiens | |
| 1.14.14.80 | 0.5 | - |
myristic acid | pH 7.4, 37°C, recombinant mutant K276T with cytochrome B5 | Homo sapiens | |
| 1.14.14.80 | 0.502 | - |
lauric acid | pH 7.4, 37°C, recombinant mutant S353G | Homo sapiens | |
| 1.14.14.80 | 0.822 | - |
myristic acid | pH 7.4, 37°C, recombinant mutant S353G with cytochrome B5 | Homo sapiens | |
| 1.14.14.80 | 0.842 | - |
lauric acid | pH 7.4, 37°C, recombinant mutant K276T with cytochrome B5 | Homo sapiens | |
| 1.14.14.80 | 0.998 | - |
lauric acid | pH 7.4, 37°C, recombinant mutant S353G with cytochrome B5 | Homo sapiens | |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.14.14.80 | 7.4 | - |
assay at | Homo sapiens |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.14.14.80 | cytochrome P450 | - |
Homo sapiens | |
| 1.14.14.80 | heme | - |
Homo sapiens | |
| 1.14.14.80 | NADPH | - |
Homo sapiens | |
kcat/KM [mM/s]
| EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.14.14.80 | 2.5 | - |
palmitic acid | pH 7.4, 37°C, recombinant wild-type enzyme | Homo sapiens | |
| 1.14.14.80 | 3.09 | - |
palmitic acid | pH 7.4, 37°C, recombinant wild-type enzyme with cytochrome B5 | Homo sapiens | |
| 1.14.14.80 | 4.76 | - |
palmitic acid | pH 7.4, 37°C, recombinant mutant K276T | Homo sapiens | |
| 1.14.14.80 | 4.8 | - |
palmitic acid | pH 7.4, 37°C, recombinant mutant S353G | Homo sapiens | |
| 1.14.14.80 | 6.04 | - |
myristic acid | pH 7.4, 37°C, recombinant wild-type enzyme | Homo sapiens | |
| 1.14.14.80 | 6.16 | - |
lauric acid | pH 7.4, 37°C, recombinant wild-type enzyme | Homo sapiens | |
| 1.14.14.80 | 6.48 | - |
palmitic acid | pH 7.4, 37°C, recombinant mutant K276T with cytochrome B5 | Homo sapiens | |
| 1.14.14.80 | 8.21 | - |
palmitic acid | pH 7.4, 37°C, recombinant mutant S353G with cytochrome B5 | Homo sapiens | |
| 1.14.14.80 | 9.27 | - |
myristic acid | pH 7.4, 37°C, recombinant mutant K276T | Homo sapiens | |
| 1.14.14.80 | 10.72 | - |
myristic acid | pH 7.4, 37°C, recombinant wild-type enzyme with cytochrome B5 | Homo sapiens | |
| 1.14.14.80 | 11.22 | - |
lauric acid | pH 7.4, 37°C, recombinant mutant K276T | Homo sapiens | |
| 1.14.14.80 | 12.27 | - |
myristic acid | pH 7.4, 37°C, recombinant mutant S353G with cytochrome B5 | Homo sapiens | |
| 1.14.14.80 | 12.29 | - |
lauric acid | pH 7.4, 37°C, recombinant wild-type enzyme, with cytochrome B5 | Homo sapiens | |
| 1.14.14.80 | 12.8 | - |
myristic acid | pH 7.4, 37°C, recombinant mutant S353G | Homo sapiens | |
| 1.14.14.80 | 13.52 | - |
myristic acid | pH 7.4, 37°C, recombinant mutant K276T with cytochrome B5 | Homo sapiens | |
| 1.14.14.80 | 22.76 | - |
lauric acid | pH 7.4, 37°C, recombinant mutant K276T with cytochrome B5 | Homo sapiens | |
| 1.14.14.80 | 23.91 | - |
lauric acid | pH 7.4, 37°C, recombinant mutant S353G | Homo sapiens | |
| 1.14.14.80 | 30.24 | - |
lauric acid | pH 7.4, 37°C, recombinant mutant S353G with cytochrome B5 | Homo sapiens | |
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