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Literature summary extracted from

  • Zhang, S.; Li, W.; Zhang, D.; Huang, X.; Qin, W.; Gu, J.
    Purification and characterization of a low-temperature ammonia monooxygenase from heterotrophic nitrifier Acinetobacter sp. Y16 (2015), Desalin. Water Treatment, 53, 257-262 .
No PubMed abstract available

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.14.99.39 membrane membrane-bound Acinetobacter sp. 16020
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.14.99.39 Cu2+ CuCl2 stimulates AMO activity in vitro Acinetobacter sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.99.39 NH3 + a reduced acceptor + O2 Acinetobacter sp.
-
 NH2OH + an acceptor + H2O
-
 ?
1.14.99.39 NH3 + a reduced acceptor + O2 Acinetobacter sp. Y16
-
 NH2OH + an acceptor + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.99.39 Acinetobacter sp.
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 isolated from Songhua River in winter, China
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1.14.99.39 Acinetobacter sp. Y16
-
 isolated from Songhua River in winter, China
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Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.99.39 native enzyme 9.7fold by by anion-exchange chromatography and gel filtration as membrane-bound monomer Acinetobacter sp.

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.14.99.39 23.2
-
 purified native enzyme, pH 7.5, 15°C Acinetobacter sp.

Storage Stability

EC Number Storage Stability Organism
1.14.99.39 4-15°C, the purified enzyme activity is stable when stored at 4°C for five days, at 10°C for two days, and at 15°C for one day Acinetobacter sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.99.39 additional information the enzyme catalyzes the oxidation of ammonium without stabilizing agents in vitro at low temperature Acinetobacter sp. ?
-
 ?
1.14.99.39 additional information the enzyme catalyzes the oxidation of ammonium without stabilizing agents in vitro at low temperature Acinetobacter sp. Y16 ?
-
 ?
1.14.99.39 NH3 + a reduced acceptor + O2
-
 Acinetobacter sp. NH2OH + an acceptor + H2O
-
 ?
1.14.99.39 NH3 + a reduced acceptor + O2
-
 Acinetobacter sp. Y16 NH2OH + an acceptor + H2O
-
 ?

Subunits

EC Number Subunits Comment Organism
1.14.99.39 monomer 1 * 31000, SDS-PAGE Acinetobacter sp.

Synonyms

EC Number Synonyms Comment Organism
1.14.99.39 AMO
-
 Acinetobacter sp.
1.14.99.39 amoA
-
 Acinetobacter sp.
1.14.99.39 low-temperature ammonia monooxygenase
-
 Acinetobacter sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.14.99.39 15
-
-
 Acinetobacter sp.

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.14.99.39 4 20 over 91% activity with in the range of 4-15°C, 15.1% of maximal activity at 20C, complete inactivation above 20°C Acinetobacter sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.14.99.39 7.5
-
 assay at Acinetobacter sp.