BRENDA - Enzyme Database

An essential role of Rieske domain oxygenase Neverland in the molting cycle of black tiger shrimp, Penaeus monodon

Sathapondecha, P.; Panyim, S.; Udomkit, A.; Comp. Biochem. Physiol. A 213, 11-19 (2017)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
1.14.19.21
DNA and amiino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, quantitative RT-PCR expression analysis
Penaeus monodon
Engineering
EC Number
Amino acid exchange
Commentary
Organism
1.14.19.21
additional information
construction and expression of double-stranded RNA specific to PmNvd (dsNvd) for silencing of the enzyme, effect of PmNvd knockdown on molting duration, phenotype, overview
Penaeus monodon
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
1.14.19.21
membrane
the enzyme sequence contains the transmembrane motif 33WxxF59
Penaeus monodon
16020
-
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
1.14.19.21
Iron
the enzyme structure contains a Rieske [2Fe-2S] domain motif 107AxxG209 with the highly conserved sequence C-X-H-X16-17-C-X2-H and a non-heme iron-binding motif E-X3-D-X2-H-X4-H
Penaeus monodon
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.14.19.21
Penaeus monodon
A0A2I4PEL8
-
-
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
1.14.19.21
additional information
the expression of PmNvd is dominantly in the Y-organ, but changes during molting period. The PmNvd mRNA level is low in intermolt and early premolt stages, then dramatically increases in the mid premolt stage. No enzyme expression in eyestalk, brain, thoracic ganglia, nerve cord, hepatopancreas, ovary, gills, muscle, and lymphoid organ, RT-PCR expression analysis
Penaeus monodon
-
1.14.19.21
Y-organ
exclusive expression
Penaeus monodon
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.14.19.21
cholesterol + O2 + NAD(P)H + H+
-
744745
Penaeus monodon
cholesta-5,7-dien-3beta-ol + NAD(P)+ + 2 H2O
-
-
-
?
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.14.19.21
[2Fe-2S]-center
the enzyme structure contains a Rieske [2Fe-2S] domain motif 107AxxG209 with the highly conserved sequence C-X-H-X16-17-C-X2-H and a non-heme iron-binding motif E-X3-D-X2-H-X4-H
Penaeus monodon
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
1.14.19.21
DNA and amiino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, quantitative RT-PCR expression analysis
Penaeus monodon
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.14.19.21
[2Fe-2S]-center
the enzyme structure contains a Rieske [2Fe-2S] domain motif 107AxxG209 with the highly conserved sequence C-X-H-X16-17-C-X2-H and a non-heme iron-binding motif E-X3-D-X2-H-X4-H
Penaeus monodon
Engineering (protein specific)
EC Number
Amino acid exchange
Commentary
Organism
1.14.19.21
additional information
construction and expression of double-stranded RNA specific to PmNvd (dsNvd) for silencing of the enzyme, effect of PmNvd knockdown on molting duration, phenotype, overview
Penaeus monodon
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
1.14.19.21
membrane
the enzyme sequence contains the transmembrane motif 33WxxF59
Penaeus monodon
16020
-
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
1.14.19.21
Iron
the enzyme structure contains a Rieske [2Fe-2S] domain motif 107AxxG209 with the highly conserved sequence C-X-H-X16-17-C-X2-H and a non-heme iron-binding motif E-X3-D-X2-H-X4-H
Penaeus monodon
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
1.14.19.21
additional information
the expression of PmNvd is dominantly in the Y-organ, but changes during molting period. The PmNvd mRNA level is low in intermolt and early premolt stages, then dramatically increases in the mid premolt stage. No enzyme expression in eyestalk, brain, thoracic ganglia, nerve cord, hepatopancreas, ovary, gills, muscle, and lymphoid organ, RT-PCR expression analysis
Penaeus monodon
-
1.14.19.21
Y-organ
exclusive expression
Penaeus monodon
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.14.19.21
cholesterol + O2 + NAD(P)H + H+
-
744745
Penaeus monodon
cholesta-5,7-dien-3beta-ol + NAD(P)+ + 2 H2O
-
-
-
?
General Information
EC Number
General Information
Commentary
Organism
1.14.19.21
malfunction
silencing of PmNvd transcript in shrimp by specific double-stranded RNA (dsNvd) leads to prolonged molt duration with abnormal molting progression, i.e. the molting process got stuck at early premolt stage. In addition, the 20-hydroxyecdysone titer in hemolymph of dsNvd-injected shrimps is significantly reduced compared with that in NaCl-injected shrimps. Phenotype, overview
Penaeus monodon
1.14.19.21
metabolism
the cholesterol 7,8-dehydrogenase catalyzes the first step of the ecdysteroid biosynthesis converting cholesterol to 7-dehydrocholesterol
Penaeus monodon
1.14.19.21
physiological function
function of the enzyme in the molting process and molt regulation, crucial role of PmNvd in molt progression, particularly during the initiation of premolt phase via the regulation of ecdysteroid production
Penaeus monodon
General Information (protein specific)
EC Number
General Information
Commentary
Organism
1.14.19.21
malfunction
silencing of PmNvd transcript in shrimp by specific double-stranded RNA (dsNvd) leads to prolonged molt duration with abnormal molting progression, i.e. the molting process got stuck at early premolt stage. In addition, the 20-hydroxyecdysone titer in hemolymph of dsNvd-injected shrimps is significantly reduced compared with that in NaCl-injected shrimps. Phenotype, overview
Penaeus monodon
1.14.19.21
metabolism
the cholesterol 7,8-dehydrogenase catalyzes the first step of the ecdysteroid biosynthesis converting cholesterol to 7-dehydrocholesterol
Penaeus monodon
1.14.19.21
physiological function
function of the enzyme in the molting process and molt regulation, crucial role of PmNvd in molt progression, particularly during the initiation of premolt phase via the regulation of ecdysteroid production
Penaeus monodon