Isolation and expression analysis of the flavanone 3-hydroxylase genes in lotus (Nelumbo nucifera Gaertn.), waterlily (Nymphaea sp.) and transient silencing in waterlily
Chaipanya, C.; Saetiew, K.; Arunyanart, S.; Parinthawong, N.; Chiang Mai J. Sci. 44, 427-437 (2017)
No PubMed abstract available
Data extracted from this reference:
Cloned(Commentary)
EC Number
Cloned (Commentary)
Organism
1.14.11.9
gene F3H, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, semi-quantitative RT-PCR enzyme expression analysis
Nelumbo nucifera
1.14.11.9
gene F3H, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, semi-quantitative RT-PCR enzyme expression analysis
Nymphaea hybrid cultivar
Engineering
EC Number
Protein Variants
Commentary
Organism
1.14.11.9
additional information
method evaluation for RNAi-mediated silencing downregulation of F3H expression, vector pJA8F3H is efficient and can be used as a transformation vector to transiently suppress F3H expression in waterlily or lotus
Nelumbo nucifera
1.14.11.9
additional information
method evaluation for RNAi-mediated silencing downregulation of F3H expression, introduction of RNAi gene-silencing vector, pJA8F3H, encoding a hairpin F3H RNA, to waterlily petals using the Agrobacterium tumefaciens strain GV3101 infiltration method, F3H expression is analysed at 1 and 3 days post infiltration semi-quantitative RT-PCR, showing that F3H expression is downregulated at 3 dpi in flowers tested of the red petal variety and purplish blue petal variety compared to controls. Vector pJA8F3H is efficient and can be used as a transformation vector to transiently suppress F3H expression in waterlily or lotus
Nymphaea hybrid cultivar
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
1.14.11.9
a (2S)-flavan-4-one + 2-oxoglutarate + O2
Nelumbo nucifera
-
a (2R,3R)-dihydroflavonol + succinate + CO2
-
-
?
1.14.11.9
a (2S)-flavan-4-one + 2-oxoglutarate + O2
Nymphaea hybrid cultivar
-
a (2R,3R)-dihydroflavonol + succinate + CO2
-
-
?
Organism
EC Number
Organism
UniProt
Commentary
Textmining
1.14.11.9
Nelumbo nucifera
V9P4G8
cv. Buntharik (white petal lotus) and cv. Satabankacha (pink petal lotus)
-
1.14.11.9
Nymphaea hybrid cultivar
V9P4H2
var. St. Louis Gold
-
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
1.14.11.9
leaf
low expression level
Nymphaea hybrid cultivar
-
1.14.11.9
additional information
expression of F3H is specifically regulated in petals and stamens. The F3H expression is slightly diminished in petals of cultivar Satabankacha at the fully-opening stage, whereas it is detected in the cultivar Buntharik only when white petals are tinted with pink.
Nelumbo nucifera
-
1.14.11.9
petal
-
Nelumbo nucifera
-
1.14.11.9
petal
-
Nymphaea hybrid cultivar
-
1.14.11.9
stamen
-
Nelumbo nucifera
-
1.14.11.9
stamen
-
Nymphaea hybrid cultivar
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
1.14.11.9
a (2S)-flavan-4-one + 2-oxoglutarate + O2
-
744729
Nelumbo nucifera
a (2R,3R)-dihydroflavonol + succinate + CO2
-
-
-
?
1.14.11.9
a (2S)-flavan-4-one + 2-oxoglutarate + O2
-
744729
Nymphaea hybrid cultivar
a (2R,3R)-dihydroflavonol + succinate + CO2
-
-
-
?
Synonyms
EC Number
Synonyms
Commentary
Organism
1.14.11.9
F3H
-
Nelumbo nucifera
1.14.11.9
F3H
-
Nymphaea hybrid cultivar
1.14.11.9
flavanone 3-hydroxylase
-
Nelumbo nucifera
1.14.11.9
flavanone 3-hydroxylase
-
Nymphaea hybrid cultivar
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
1.14.11.9
gene F3H, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, semi-quantitative RT-PCR enzyme expression analysis
Nelumbo nucifera
1.14.11.9
gene F3H, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, semi-quantitative RT-PCR enzyme expression analysis
Nymphaea hybrid cultivar
Engineering (protein specific)
EC Number
Protein Variants
Commentary
Organism
1.14.11.9
additional information
method evaluation for RNAi-mediated silencing downregulation of F3H expression, vector pJA8F3H is efficient and can be used as a transformation vector to transiently suppress F3H expression in waterlily or lotus
Nelumbo nucifera
1.14.11.9
additional information
method evaluation for RNAi-mediated silencing downregulation of F3H expression, introduction of RNAi gene-silencing vector, pJA8F3H, encoding a hairpin F3H RNA, to waterlily petals using the Agrobacterium tumefaciens strain GV3101 infiltration method, F3H expression is analysed at 1 and 3 days post infiltration semi-quantitative RT-PCR, showing that F3H expression is downregulated at 3 dpi in flowers tested of the red petal variety and purplish blue petal variety compared to controls. Vector pJA8F3H is efficient and can be used as a transformation vector to transiently suppress F3H expression in waterlily or lotus
Nymphaea hybrid cultivar
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
1.14.11.9
a (2S)-flavan-4-one + 2-oxoglutarate + O2
Nelumbo nucifera
-
a (2R,3R)-dihydroflavonol + succinate + CO2
-
-
?
1.14.11.9
a (2S)-flavan-4-one + 2-oxoglutarate + O2
Nymphaea hybrid cultivar
-
a (2R,3R)-dihydroflavonol + succinate + CO2
-
-
?
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
1.14.11.9
leaf
low expression level
Nymphaea hybrid cultivar
-
1.14.11.9
additional information
expression of F3H is specifically regulated in petals and stamens. The F3H expression is slightly diminished in petals of cultivar Satabankacha at the fully-opening stage, whereas it is detected in the cultivar Buntharik only when white petals are tinted with pink.
Nelumbo nucifera
-
1.14.11.9
petal
-
Nelumbo nucifera
-
1.14.11.9
petal
-
Nymphaea hybrid cultivar
-
1.14.11.9
stamen
-
Nelumbo nucifera
-
1.14.11.9
stamen
-
Nymphaea hybrid cultivar
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
1.14.11.9
a (2S)-flavan-4-one + 2-oxoglutarate + O2
-
744729
Nelumbo nucifera
a (2R,3R)-dihydroflavonol + succinate + CO2
-
-
-
?
1.14.11.9
a (2S)-flavan-4-one + 2-oxoglutarate + O2
-
744729
Nymphaea hybrid cultivar
a (2R,3R)-dihydroflavonol + succinate + CO2
-
-
-
?
General Information
EC Number
General Information
Commentary
Organism
1.14.11.9
physiological function
F3H is a key regulator for the flavonoid biosynthesis pathway and plays a role in accumulation of anthocyanin pigments in the vacuole
Nelumbo nucifera
1.14.11.9
physiological function
F3H is a key regulator for the flavonoid biosynthesis pathway and plays a role in accumulation of anthocyanin pigments in the vacuole
Nymphaea hybrid cultivar
General Information (protein specific)
EC Number
General Information
Commentary
Organism
1.14.11.9
physiological function
F3H is a key regulator for the flavonoid biosynthesis pathway and plays a role in accumulation of anthocyanin pigments in the vacuole
Nelumbo nucifera
1.14.11.9
physiological function
F3H is a key regulator for the flavonoid biosynthesis pathway and plays a role in accumulation of anthocyanin pigments in the vacuole
Nymphaea hybrid cultivar