BRENDA - Enzyme Database

Unusually broad substrate profile of self-sufficient Cytochrome P450 monooxygenase CYP116B4 from Labrenzia aggregata

Yin, Y.; Yu, H.; Luan, Z.; Li, R.; Ouyang, P.; Liu, J.; Xu, J.; ChemBioChem 15, 2443-2449 (2014)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
1.14.14.1
expression in Escherichia coli
Labrenzia aggregata
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.14.14.1
Labrenzia aggregata
A0P0F6
-
-
1.14.14.1
Labrenzia aggregata DSM 13394
A0P0F6
-
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.14.14.1
(methylsulfanyl)benzene + [reduced NADPH-hemoprotein reductase] + H+ + O2
-
744697
Labrenzia aggregata
methyl(phenyl)sulfaniumolate + [oxidized NADPH-hemoprotein reductase] + ?
-
-
-
?
1.14.14.1
(methylsulfanyl)benzene + [reduced NADPH-hemoprotein reductase] + H+ + O2
-
744697
Labrenzia aggregata DSM 13394
methyl(phenyl)sulfaniumolate + [oxidized NADPH-hemoprotein reductase] + ?
-
-
-
?
1.14.14.1
1-chloro-4-(methylsulfanyl)benzene + [reduced NADPH-hemoprotein reductase] + H+ + O2
-
744697
Labrenzia aggregata
(4-chlorophenyl)(methyl)sulfaniumolate + [oxidized NADPH-hemoprotein reductase] + ?
-
-
-
?
1.14.14.1
1-chloro-4-(methylsulfanyl)benzene + [reduced NADPH-hemoprotein reductase] + H+ + O2
-
744697
Labrenzia aggregata DSM 13394
(4-chlorophenyl)(methyl)sulfaniumolate + [oxidized NADPH-hemoprotein reductase] + ?
-
-
-
?
1.14.14.1
1-chloro-4-ethenylbenzene + [reduced NADPH-hemoprotein reductase] + H+ + O2
-
744697
Labrenzia aggregata
(4-chlorophenyl)acetaldehyde + 2-(4-chlorophenyl)oxirane + 2-(4-chlorophenyl)ethan-1-ol + [oxidized NADPH-hemoprotein reductase] + ?
-
-
-
?
1.14.14.1
1-chloro-4-ethenylbenzene + [reduced NADPH-hemoprotein reductase] + H+ + O2
-
744697
Labrenzia aggregata DSM 13394
(4-chlorophenyl)acetaldehyde + 2-(4-chlorophenyl)oxirane + 2-(4-chlorophenyl)ethan-1-ol + [oxidized NADPH-hemoprotein reductase] + ?
-
-
-
?
1.14.14.1
1-methoxy-4-(methylsulfanyl)benzene + [reduced NADPH-hemoprotein reductase] + H+ + O2
-
744697
Labrenzia aggregata
(4-methoxyphenyl)(methyl)sulfaniumolate + [oxidized NADPH-hemoprotein reductase] + ?
-
-
-
?
1.14.14.1
1-methoxy-4-(methylsulfanyl)benzene + [reduced NADPH-hemoprotein reductase] + H+ + O2
-
744697
Labrenzia aggregata DSM 13394
(4-methoxyphenyl)(methyl)sulfaniumolate + [oxidized NADPH-hemoprotein reductase] + ?
-
-
-
?
1.14.14.1
2,3-dihydroindene + [reduced NADPH-hemoprotein reductase] + H+ + O2
-
744697
Labrenzia aggregata
(1S)-2,3-dihydroinden-1-ol + 2,3-dihydroinden-1-one + [oxidized NADPH-hemoprotein reductase] + ?
-
-
-
?
1.14.14.1
4-chlorothioanisole + [reduced NADPH-hemoprotein reductase] + H+ + O2
-
744697
Labrenzia aggregata
4-chlorothioanisole sulfoxide + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
1.14.14.1
4-nitroanisole + [reduced NADPH-hemoprotein reductase] + H+ + O2
-
744697
Labrenzia aggregata
4-nitrophenol + [oxidized NADPH-hemoprotein reductase] + ?
-
-
-
?
1.14.14.1
7-ethoxycoumarin + [reduced NADPH-hemoprotein reductase] + H+
-
744697
Labrenzia aggregata
coumarin + [oxidized NADPH-hemoprotein reductase] + ?
-
-
-
?
1.14.14.1
7-methoxycoumarin + [reduced NADPH-hemoprotein reductase] + H+
-
744697
Labrenzia aggregata
coumarin + [oxidized NADPH-hemoprotein reductase] + ?
-
-
-
?
1.14.14.1
cytochrome c + O2 + NADH
-
744697
Labrenzia aggregata
? + NAD+ + ?
-
-
-
?
1.14.14.1
ethylbenzene + [reduced NADPH-hemoprotein reductase] + H+ + O2
-
744697
Labrenzia aggregata
(1S)-1-phenylethan-1-ol + 1-phenylethan-1-one + [oxidized NADPH-hemoprotein reductase] + ?
-
-
-
?
1.14.14.1
indene + [reduced NADPH-hemoprotein reductase] + H+ + O2
-
744697
Labrenzia aggregata
(S)-inden-1-ol + 2,3-dihydroinden-1-one + [oxidized NADPH-hemoprotein reductase] + ?
-
-
-
?
1.14.14.1
additional information
versatile catalyst, exhibiting hydroxylation and epoxidation activities as well as O-dealkylation and asymmetric sulfoxidation activities. Alkylbenzenes, aromatic bicyclic molecules, and terpenoids are hydroxylated by Cyp116B4
744697
Labrenzia aggregata
?
-
-
-
-
1.14.14.1
additional information
versatile catalyst, exhibiting hydroxylation and epoxidation activities as well as O-dealkylation and asymmetric sulfoxidation activities. Alkylbenzenes, aromatic bicyclic molecules, and terpenoids are hydroxylated by Cyp116B4
744697
Labrenzia aggregata DSM 13394
?
-
-
-
-
1.14.14.1
toluene + [reduced NADPH-hemoprotein reductase] + H+ + O2
-
744697
Labrenzia aggregata
benzylalcohol + benzaldehyde + [oxidized NADPH-hemoprotein reductase] + ?
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
1.14.14.1
?
x * 87000, SDS-PAGE
Labrenzia aggregata
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.14.14.1
cytochrome P450
-
Labrenzia aggregata
1.14.14.1
FMN
-
Labrenzia aggregata
1.14.14.1
NADH
NADPH is preferred over NADH
Labrenzia aggregata
1.14.14.1
NADPH
NADPH is preferred over NADH
Labrenzia aggregata
1.14.14.1
[2Fe-2S]-center
-
Labrenzia aggregata
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
1.14.14.1
expression in Escherichia coli
Labrenzia aggregata
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.14.14.1
cytochrome P450
-
Labrenzia aggregata
1.14.14.1
FMN
-
Labrenzia aggregata
1.14.14.1
NADH
NADPH is preferred over NADH
Labrenzia aggregata
1.14.14.1
NADPH
NADPH is preferred over NADH
Labrenzia aggregata
1.14.14.1
[2Fe-2S]-center
-
Labrenzia aggregata
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.14.14.1
(methylsulfanyl)benzene + [reduced NADPH-hemoprotein reductase] + H+ + O2
-
744697
Labrenzia aggregata
methyl(phenyl)sulfaniumolate + [oxidized NADPH-hemoprotein reductase] + ?
-
-
-
?
1.14.14.1
(methylsulfanyl)benzene + [reduced NADPH-hemoprotein reductase] + H+ + O2
-
744697
Labrenzia aggregata DSM 13394
methyl(phenyl)sulfaniumolate + [oxidized NADPH-hemoprotein reductase] + ?
-
-
-
?
1.14.14.1
1-chloro-4-(methylsulfanyl)benzene + [reduced NADPH-hemoprotein reductase] + H+ + O2
-
744697
Labrenzia aggregata
(4-chlorophenyl)(methyl)sulfaniumolate + [oxidized NADPH-hemoprotein reductase] + ?
-
-
-
?
1.14.14.1
1-chloro-4-(methylsulfanyl)benzene + [reduced NADPH-hemoprotein reductase] + H+ + O2
-
744697
Labrenzia aggregata DSM 13394
(4-chlorophenyl)(methyl)sulfaniumolate + [oxidized NADPH-hemoprotein reductase] + ?
-
-
-
?
1.14.14.1
1-chloro-4-ethenylbenzene + [reduced NADPH-hemoprotein reductase] + H+ + O2
-
744697
Labrenzia aggregata
(4-chlorophenyl)acetaldehyde + 2-(4-chlorophenyl)oxirane + 2-(4-chlorophenyl)ethan-1-ol + [oxidized NADPH-hemoprotein reductase] + ?
-
-
-
?
1.14.14.1
1-chloro-4-ethenylbenzene + [reduced NADPH-hemoprotein reductase] + H+ + O2
-
744697
Labrenzia aggregata DSM 13394
(4-chlorophenyl)acetaldehyde + 2-(4-chlorophenyl)oxirane + 2-(4-chlorophenyl)ethan-1-ol + [oxidized NADPH-hemoprotein reductase] + ?
-
-
-
?
1.14.14.1
1-methoxy-4-(methylsulfanyl)benzene + [reduced NADPH-hemoprotein reductase] + H+ + O2
-
744697
Labrenzia aggregata
(4-methoxyphenyl)(methyl)sulfaniumolate + [oxidized NADPH-hemoprotein reductase] + ?
-
-
-
?
1.14.14.1
1-methoxy-4-(methylsulfanyl)benzene + [reduced NADPH-hemoprotein reductase] + H+ + O2
-
744697
Labrenzia aggregata DSM 13394
(4-methoxyphenyl)(methyl)sulfaniumolate + [oxidized NADPH-hemoprotein reductase] + ?
-
-
-
?
1.14.14.1
2,3-dihydroindene + [reduced NADPH-hemoprotein reductase] + H+ + O2
-
744697
Labrenzia aggregata
(1S)-2,3-dihydroinden-1-ol + 2,3-dihydroinden-1-one + [oxidized NADPH-hemoprotein reductase] + ?
-
-
-
?
1.14.14.1
4-chlorothioanisole + [reduced NADPH-hemoprotein reductase] + H+ + O2
-
744697
Labrenzia aggregata
4-chlorothioanisole sulfoxide + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
1.14.14.1
4-nitroanisole + [reduced NADPH-hemoprotein reductase] + H+ + O2
-
744697
Labrenzia aggregata
4-nitrophenol + [oxidized NADPH-hemoprotein reductase] + ?
-
-
-
?
1.14.14.1
7-ethoxycoumarin + [reduced NADPH-hemoprotein reductase] + H+
-
744697
Labrenzia aggregata
coumarin + [oxidized NADPH-hemoprotein reductase] + ?
-
-
-
?
1.14.14.1
7-methoxycoumarin + [reduced NADPH-hemoprotein reductase] + H+
-
744697
Labrenzia aggregata
coumarin + [oxidized NADPH-hemoprotein reductase] + ?
-
-
-
?
1.14.14.1
cytochrome c + O2 + NADH
-
744697
Labrenzia aggregata
? + NAD+ + ?
-
-
-
?
1.14.14.1
ethylbenzene + [reduced NADPH-hemoprotein reductase] + H+ + O2
-
744697
Labrenzia aggregata
(1S)-1-phenylethan-1-ol + 1-phenylethan-1-one + [oxidized NADPH-hemoprotein reductase] + ?
-
-
-
?
1.14.14.1
indene + [reduced NADPH-hemoprotein reductase] + H+ + O2
-
744697
Labrenzia aggregata
(S)-inden-1-ol + 2,3-dihydroinden-1-one + [oxidized NADPH-hemoprotein reductase] + ?
-
-
-
?
1.14.14.1
additional information
versatile catalyst, exhibiting hydroxylation and epoxidation activities as well as O-dealkylation and asymmetric sulfoxidation activities. Alkylbenzenes, aromatic bicyclic molecules, and terpenoids are hydroxylated by Cyp116B4
744697
Labrenzia aggregata
?
-
-
-
-
1.14.14.1
additional information
versatile catalyst, exhibiting hydroxylation and epoxidation activities as well as O-dealkylation and asymmetric sulfoxidation activities. Alkylbenzenes, aromatic bicyclic molecules, and terpenoids are hydroxylated by Cyp116B4
744697
Labrenzia aggregata DSM 13394
?
-
-
-
-
1.14.14.1
toluene + [reduced NADPH-hemoprotein reductase] + H+ + O2
-
744697
Labrenzia aggregata
benzylalcohol + benzaldehyde + [oxidized NADPH-hemoprotein reductase] + ?
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
1.14.14.1
?
x * 87000, SDS-PAGE
Labrenzia aggregata