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Literature summary extracted from
- Bestimmung kinetischer Parameter der FADH2-abhängigen Tryptophan-5-Halogenase aus Streptomyces rugosporus (2010), Chem.-Ing.-Tech., 82, 121-127 .
No PubMed abstract available
Application
| EC Number | Application | Comment | Organism |
|---|---|---|---|
| 1.14.19.58 | synthesis | regeneration of FADH2 using flavin dehydrogenase plus formiate dehydrogenase from Candida boidiniiat 0.28 U/ml leads to about 60% increase in yield | Streptomyces rugosporus |
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 1.14.19.58 | expression in Escherichia coli | Streptomyces rugosporus |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.14.19.58 | additional information | no substrate inhibition by tryptophan up to 2 mM | Streptomyces rugosporus |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.14.19.58 | 0.071 | - |
L-tryptophan | pH 7.2, 30°C | Streptomyces rugosporus |  |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.14.19.58 | Streptomyces rugosporus | A4D0H5 | - |
- |
| 1.14.19.58 | Streptomyces rugosporus LL-42D005 | A4D0H5 | - |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.14.19.58 | recombinant protein | Streptomyces rugosporus |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.14.19.58 | L-tryptophan + FADH2 + chloride + O2 + H+ | - |
Streptomyces rugosporus | 5-chloro-L-tryptophan + FAD + 2 H2O | - |
? | |
| 1.14.19.58 | L-tryptophan + FADH2 + chloride + O2 + H+ | - |
Streptomyces rugosporus LL-42D005 | 5-chloro-L-tryptophan + FAD + 2 H2O | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 1.14.19.58 | pyrH | - |
Streptomyces rugosporus |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.14.19.58 | - |
- |
half-life 1848 min | Streptomyces rugosporus |
| 1.14.19.58 | 22 | - |
half-life 517 min | Streptomyces rugosporus |
| 1.14.19.58 | 30 | - |
half-life 43 min | Streptomyces rugosporus |
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Release 2023.1 (January 2023)
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