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Literature summary extracted from

  • Gul Guven, R.; Aslan, N.; Guven, K.; Matpan Bekler, F.; Acer, O.
    Purification and characterization of polyphenol oxidase from corn tassel (2016), Cell. Mol. Biol., 62, 6-11 .
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.10.3.1 D-fructose 17% activation at 2 mM Zea mays
1.10.3.1 D-glucose 16% activation at 2 mM Zea mays
1.10.3.1 sucrose 4% activation at 2 mM Zea mays

Application

EC Number Application Comment Organism
1.10.3.1 food industry inhibition of catechol oxidase diminishes browning in white radish and elevates food quality Zea mays

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.10.3.1 EDTA 22.2% inhibition at 2 mM Zea mays
1.10.3.1 SDS 12.2% inhibition at 2 mM Zea mays
1.10.3.1 Sodium azide 26.6% inhibition at 2 mM Zea mays

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.10.3.1 additional information
-
additional information Michaelis-Menten kinetics Zea mays
1.10.3.1 3.48
-
catechol pH 8.0, 40°C Zea mays
1.10.3.1 8
-
4-methylcatechol pH 6.0, 30°C Zea mays

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.10.3.1 158000
-
native PAGE Zea mays

Organism

EC Number Organism UniProt Comment Textmining
1.10.3.1 Zea mays
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.10.3.1 native enzyme from corn tassel 10fold by ammonium sulfate fractionation and gel filtration Zea mays

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.10.3.1 tassel
-
Zea mays
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.10.3.1 additional information
-
2612.06 U/mg, one enzyme unit is defined as the amount of enzyme that produces a rise of 0.001 absorbance in one minute at 420 nm Zea mays

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.10.3.1 2 4-methylcatechol + O2
-
Zea mays 2 4-methyl-1,2-benzoquinone
-
?
1.10.3.1 2 catechol + O2 best substrate Zea mays 2 1,2-benzoquinone + 2 H2O
-
?

Subunits

EC Number Subunits Comment Organism
1.10.3.1 ? x * 158000, SDS-PAGE Zea mays

Synonyms

EC Number Synonyms Comment Organism
1.10.3.1 polyphenol oxidase
-
Zea mays
1.10.3.1 PPO
-
Zea mays

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.10.3.1 30
-
with substrate 4-methylcatechol Zea mays
1.10.3.1 40
-
with substrate catechol Zea mays

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.10.3.1 6
-
with substrate 4-methylcatechol Zea mays
1.10.3.1 8
-
with substrate catechol Zea mays