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Literature summary extracted from

  • Mai, Z.; Su, H.; Zhang, S.
    Characterization of a metagenome-derived beta-glucosidase and its application in conversion of polydatin to resveratrol (2016), Catalysts, 6, 35 .
No PubMed abstract available

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.21 expression in Escherichia coli unidentified microorganism

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.2.1.21 molecular modeling of structure. Residues Asp333 and Glu530 may act as the catalytic nucleophile and acid/base, respectively. Residues Val87, Leu187, Thr368, and Trp474 may participate in substrate recognition and binding unidentified microorganism

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.21 Ba2+ 1 mM, 44% residual activity unidentified microorganism
3.2.1.21 Ca2+ 1 mM, 58% residual activity unidentified microorganism
3.2.1.21 Cu2+ 1 mM, 8% residual activity unidentified microorganism
3.2.1.21 EDTA 1 mM, 56% residual activity unidentified microorganism
3.2.1.21 SDS 1 mM, 76% residual activity unidentified microorganism
3.2.1.21 Zn2+ 1 mM, 12% residual activity unidentified microorganism

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.21 unidentified microorganism
-
 metagenomic library of mangrove soil
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.21 127
-
 substrate esculin, pH 7.0, 40°C unidentified microorganism
3.2.1.21 248
-
 substrate 4-nitrophenyl beta-D-glucopyranoside, pH 7.0, 40°C unidentified microorganism
3.2.1.21 422
-
 substrate polydatin, pH 7.0, 40°C unidentified microorganism

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.21 4-nitrophenyl beta-D-glucopyranoside + H2O
-
 unidentified microorganism 4-nitrophenol + beta-D-glucose
-
 ?
3.2.1.21 esculin + H2O
-
 unidentified microorganism beta-D-glucose + esculetin
-
 ?
3.2.1.21 additional information enzyme exhibits weak activities toward 4-nitrophenyl beta-D-galactopyranoside and 4-nitrophenyl beta-D-xylopyranoside, but no detectable activity toward 4-nitrophenyl beta-D-cellobioside unidentified microorganism ?
-
 ?
3.2.1.21 polydatin + H2O
-
 unidentified microorganism D-glucose + 3,4',5-trihydroxystilbene
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.21 ? x * 71000, SDS-PAGE, recombinant protein unidentified microorganism

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.21 40
-
-
 unidentified microorganism

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.21 25 35 more than 50% of maximum activity unidentified microorganism

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.21 989
-
 polydatin pH 7.0, 40°C unidentified microorganism

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.21 7
-
-
 unidentified microorganism

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.2.1.21 1476
-
 polydatin pH 7.0, 40°C unidentified microorganism