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Literature summary extracted from

  • Mangan, D.; Cornaggia, C.; Liadova, A.; McCormack, N.; Ivory, R.; McKie, V.A.; Ormerod, A.; McCleary, B.V.
    Novel substrates for the automated and manual assay of endo-1,4-beta-xylanase (2017), Carbohydr. Res., 445, 14-22 .
    View publication on PubMed

Application

EC Number Application Comment Organism
3.2.1.8 analysis enzyme coupled assay procedure, using substrate 4-nitrophenyl 4,6-O-(3-oxobutylidene)-beta-D-glucosyl-(1->4)-[(1->4)-beta-D-xylopentaoside] and release of 4-nitrophenol by beta-xylosidase Aspergillus niger
3.2.1.8 analysis enzyme coupled assay procedure, using substrate 4-nitrophenyl 4,6-O-(3-oxobutylidene)-beta-D-glucosyl-(1->4)-[(1->4)-beta-D-xylopentaoside] and release of 4-nitrophenol by beta-xylosidase Neocallimastix patriciarum
3.2.1.8 analysis enzyme coupled assay procedure, using substrate 4-nitrophenyl 4,6-O-(3-oxobutylidene)-beta-D-glucosyl-(1->4)-[(1->4)-beta-D-xylopentaoside] and release of 4-nitrophenol by beta-xylosidase Cellvibrio mixtus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.8 0.166
-
 4-nitrophenyl 4,6-O-(3-oxobutylidene)-beta-D-glucosyl-(1->4)-[(1->4)-beta-D-xylopentaoside] pH 6.0, 50°C Neocallimastix patriciarum

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.8 Aspergillus niger P55329
-
-
3.2.1.8 Cellvibrio mixtus O68541
-
-
3.2.1.8 Neocallimastix patriciarum P29127
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.8 4-nitrophenyl 4,6-O-(3-oxobutylidene)-beta-D-glucosyl-(1->4)-[(1->4)-beta-D-xylopentaoside] + H2O
-
 Aspergillus niger ?
-
 ?
3.2.1.8 4-nitrophenyl 4,6-O-(3-oxobutylidene)-beta-D-glucosyl-(1->4)-[(1->4)-beta-D-xylopentaoside] + H2O
-
 Neocallimastix patriciarum ?
-
 ?
3.2.1.8 4-nitrophenyl 4,6-O-(3-oxobutylidene)-beta-D-glucosyl-(1->4)-[(1->4)-beta-D-xylopentaoside] + H2O
-
 Cellvibrio mixtus ?
-
 ?
3.2.1.8 beechwood xylan + H2O 136% of the activity with xyloheptaose Cellvibrio mixtus ?
-
 ?
3.2.1.8 beechwood xylan + H2O 161% of the activity with xyloheptaose Neocallimastix patriciarum ?
-
 ?
3.2.1.8 beechwood xylan + H2O 91% of the activity with xyloheptaose Aspergillus niger ?
-
 ?
3.2.1.8 additional information no substrate: xylotriose Aspergillus niger ?
-
 ?
3.2.1.8 additional information no substrate: xylotriose Neocallimastix patriciarum ?
-
 ?
3.2.1.8 additional information no substrate: xylotriose Cellvibrio mixtus ?
-
 ?
3.2.1.8 wheat arabinoxylan + H2O 117% of the activity with xyloheptaose Neocallimastix patriciarum ?
-
 ?
3.2.1.8 wheat arabinoxylan + H2O 48% of the activity with xyloheptaose Cellvibrio mixtus ?
-
 ?
3.2.1.8 wheat arabinoxylan + H2O 84% of the activity with xyloheptaose Aspergillus niger ?
-
 ?
3.2.1.8 xyloheptaose + H2O
-
 Aspergillus niger ?
-
 ?
3.2.1.8 xyloheptaose + H2O
-
 Neocallimastix patriciarum ?
-
 ?
3.2.1.8 xyloheptaose + H2O
-
 Cellvibrio mixtus ?
-
 ?
3.2.1.8 xylohexaose + H2O 81% of the activity with xyloheptaose Aspergillus niger ?
-
 ?
3.2.1.8 xylohexaose + H2O 98% of the activity with xyloheptaose Neocallimastix patriciarum ?
-
 ?
3.2.1.8 xylohexaose + H2O 99% of the activity with xyloheptaose Cellvibrio mixtus ?
-
 ?
3.2.1.8 xylopentaose + H2O 45% of the activity with xyloheptaose Aspergillus niger ?
-
 ?
3.2.1.8 xylopentaose + H2O 49% of the activity with xyloheptaose Neocallimastix patriciarum ?
-
 ?
3.2.1.8 xylopentaose + H2O 54% of the activity with xyloheptaose Cellvibrio mixtus ?
-
 ?
3.2.1.8 xylotetraose + H2O 1% of the activity with xyloheptaose Neocallimastix patriciarum ?
-
 ?
3.2.1.8 xylotetraose + H2O 14% of the activity with xyloheptaose Cellvibrio mixtus ?
-
 ?
3.2.1.8 xylotetraose + H2O 8% of the activity with xyloheptaose Aspergillus niger ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.8 XynA
-
 Aspergillus niger
3.2.1.8 XynA
-
 Neocallimastix patriciarum
3.2.1.8 XynC
-
 Cellvibrio mixtus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.8 50
-
-
 Neocallimastix patriciarum
3.2.1.8 50
-
-
 Cellvibrio mixtus
3.2.1.8 60
-
-
 Aspergillus niger

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.8 4.5
-
-
 Aspergillus niger
3.2.1.8 6
-
-
 Neocallimastix patriciarum
3.2.1.8 6.5
-
-
 Cellvibrio mixtus