BRENDA - Enzyme Database

Molecular characterization of flavanone 3-hydroxylase gene and flavonoid accumulation in two chemotyped safflower lines in response to methyl jasmonate stimulation

Tu, Y.; Liu, F.; Guo, D.; Fan, L.; Zhu, Z.; Xue, Y.; Gao, Y.; Guo, M.; BMC Plant Biol. 16, 132 (2016)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Cloned (Commentary)
Organism
1.14.11.9
gene CtF3H, DNA and amino acid sequence determination and analysis, phylogenic analysis, quantitative real-time PCR enzyme expression analysis, functional recombinant expression of MBP-tagged enzyme in Escherichia coli BL21(DE3)pLysS from vector pMAL-C5x leading to production of dihydrokaempferol when naringenin is available the substrate. Recombinant expression of GFP-tagged enzyme in onion cells via transfromation of Agrobacterium tumefaciens strain GV3101, where it is localized both in the nucleus and in the cytosol
Carthamus tinctorius
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
1.14.11.9
cytosol
-
Carthamus tinctorius
5829
-
1.14.11.9
additional information
subcellular localization study
Carthamus tinctorius
-
-
1.14.11.9
nucleus
-
Carthamus tinctorius
5634
-
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
1.14.11.9
Fe2+
dependent on, required for catalysis, the ferrous iron binding site HxDxnH is formed by His78, His121, His211, Asp213, His221, Asp 223, His265, His267, and His279
Carthamus tinctorius
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
1.14.11.9
a (2S)-flavan-4-one + 2-oxoglutarate + O2
Carthamus tinctorius
-
a (2R,3R)-dihydroflavonol + succinate + CO2
-
-
?
Organism
EC Number
Organism
UniProt
Commentary
Textmining
1.14.11.9
Carthamus tinctorius
M9MTF3
a quinochalcone-type safflower line with orange-yellow flowers and a flavonol-type safflower line with white flowers, i.e. ZHH0119 line and XHH007 line, respectively
-
Purification (Commentary)
EC Number
Purification (Commentary)
Organism
1.14.11.9
recombinant MBP-tagged enzyme from Escherichia coli BL21(DE3)pLysS by amylose affinity chromatography
Carthamus tinctorius
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
1.14.11.9
flower
the transcriptome expression of CtF3H shows a diametrically opposed expression pattern in a quinochalcone-type safflower line (with orange-yellow flowers) and a flavonol-type safflower line (with white flowers) under external stimulation by methyl jasmonate (MeJA), which has been identified as an elicitor of flavonoid metabolites. High expression of CtF3H in quinochalcone-type safflower line
Carthamus tinctorius
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
1.14.11.9
a (2S)-flavan-4-one + 2-oxoglutarate + O2
-
744604
Carthamus tinctorius
a (2R,3R)-dihydroflavonol + succinate + CO2
-
-
-
?
1.14.11.9
a (2S)-flavan-4-one + 2-oxoglutarate + O2
the 2-oxoglutarate binding site RxS is formed by Arg289 and Ser291
744604
Carthamus tinctorius
a (2R,3R)-dihydroflavonol + succinate + CO2
-
-
-
?
1.14.11.9
additional information
no activity with kaempferol, dihydrokaempferol, quercetin, and dihydroquercetin
744604
Carthamus tinctorius
?
-
-
-
-
1.14.11.9
naringenin + 2-oxoglutarate + O2
-
744604
Carthamus tinctorius
dihydroquercetin + succinate + CO2
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
1.14.11.9
?
x * 40720, sequence calculation, x * 40000, recombinant detagged enzyme, SDS-PAGE
Carthamus tinctorius
Synonyms
EC Number
Synonyms
Commentary
Organism
1.14.11.9
CtF3H
-
Carthamus tinctorius
1.14.11.9
F3H
-
Carthamus tinctorius
1.14.11.9
flavanone 3-hydroxylase
-
Carthamus tinctorius
pI Value
EC Number
Organism
Commentary
pI Value Maximum
pI Value
1.14.11.9
Carthamus tinctorius
sequence calculation
-
5.57
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
1.14.11.9
gene CtF3H, DNA and amino acid sequence determination and analysis, phylogenic analysis, quantitative real-time PCR enzyme expression analysis, functional recombinant expression of MBP-tagged enzyme in Escherichia coli BL21(DE3)pLysS from vector pMAL-C5x leading to production of dihydrokaempferol when naringenin is available the substrate. Recombinant expression of GFP-tagged enzyme in onion cells via transfromation of Agrobacterium tumefaciens strain GV3101, where it is localized both in the nucleus and in the cytosol
Carthamus tinctorius
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
1.14.11.9
cytosol
-
Carthamus tinctorius
5829
-
1.14.11.9
additional information
subcellular localization study
Carthamus tinctorius
-
-
1.14.11.9
nucleus
-
Carthamus tinctorius
5634
-
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
1.14.11.9
Fe2+
dependent on, required for catalysis, the ferrous iron binding site HxDxnH is formed by His78, His121, His211, Asp213, His221, Asp 223, His265, His267, and His279
Carthamus tinctorius
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
1.14.11.9
a (2S)-flavan-4-one + 2-oxoglutarate + O2
Carthamus tinctorius
-
a (2R,3R)-dihydroflavonol + succinate + CO2
-
-
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.14.11.9
recombinant MBP-tagged enzyme from Escherichia coli BL21(DE3)pLysS by amylose affinity chromatography
Carthamus tinctorius
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
1.14.11.9
flower
the transcriptome expression of CtF3H shows a diametrically opposed expression pattern in a quinochalcone-type safflower line (with orange-yellow flowers) and a flavonol-type safflower line (with white flowers) under external stimulation by methyl jasmonate (MeJA), which has been identified as an elicitor of flavonoid metabolites. High expression of CtF3H in quinochalcone-type safflower line
Carthamus tinctorius
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
1.14.11.9
a (2S)-flavan-4-one + 2-oxoglutarate + O2
-
744604
Carthamus tinctorius
a (2R,3R)-dihydroflavonol + succinate + CO2
-
-
-
?
1.14.11.9
a (2S)-flavan-4-one + 2-oxoglutarate + O2
the 2-oxoglutarate binding site RxS is formed by Arg289 and Ser291
744604
Carthamus tinctorius
a (2R,3R)-dihydroflavonol + succinate + CO2
-
-
-
?
1.14.11.9
additional information
no activity with kaempferol, dihydrokaempferol, quercetin, and dihydroquercetin
744604
Carthamus tinctorius
?
-
-
-
-
1.14.11.9
naringenin + 2-oxoglutarate + O2
-
744604
Carthamus tinctorius
dihydroquercetin + succinate + CO2
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
1.14.11.9
?
x * 40720, sequence calculation, x * 40000, recombinant detagged enzyme, SDS-PAGE
Carthamus tinctorius
pI Value (protein specific)
EC Number
Organism
Commentary
pI Value Maximum
pI Value
1.14.11.9
Carthamus tinctorius
sequence calculation
-
5.57
Expression
EC Number
Organism
Commentary
Expression
1.14.11.9
Carthamus tinctorius
the enzyme flavanone 3-hydroxylase is induced by methyl jasmonate. Further metabolite analysis shows the increasing tendency of quinochalcones and flavonols, such as hydroxysafflor yellow A, kaempferol-3-O-beta-D-glucoside, kaempferol-3-O-beta-rutinoside, rutin, carthamin, and luteolin, in the quinochalcone-type safflower line. Also, the accumulation of kaempferol-3-O-beta-rutinoside and kaempferol-3-O-beta-D-glucoside in flavonols-typed safflower line shows enhanced accumulation pattern after methyl jasmonate treatment. Other flavonols, such as kaempferol, dihydrokaempferol and quercetin-3-O-beta-D-glucoside, in flavonols-typed safflower line present downregulation in respons to the methyl jasmonate stimulus
up
General Information
EC Number
General Information
Commentary
Organism
1.14.11.9
evolution
the enzyme belongs to the Fe2+/2–oxoglutarate-dependent dioxygenase superfamily
Carthamus tinctorius
1.14.11.9
physiological function
flavanone 3-hydroxylase (F3H) is one of the nuclear enzymes acting at the bifurcation of the flavonoid biosynthetic pathway, initiating catalysis of the 3-hydroxylation of (2S)-flavanones, such as naringenin to dihydroflavonols
Carthamus tinctorius
General Information (protein specific)
EC Number
General Information
Commentary
Organism
1.14.11.9
evolution
the enzyme belongs to the Fe2+/2–oxoglutarate-dependent dioxygenase superfamily
Carthamus tinctorius
1.14.11.9
physiological function
flavanone 3-hydroxylase (F3H) is one of the nuclear enzymes acting at the bifurcation of the flavonoid biosynthetic pathway, initiating catalysis of the 3-hydroxylation of (2S)-flavanones, such as naringenin to dihydroflavonols
Carthamus tinctorius
Expression (protein specific)
EC Number
Organism
Commentary
Expression
1.14.11.9
Carthamus tinctorius
the enzyme flavanone 3-hydroxylase is induced by methyl jasmonate. Further metabolite analysis shows the increasing tendency of quinochalcones and flavonols, such as hydroxysafflor yellow A, kaempferol-3-O-beta-D-glucoside, kaempferol-3-O-beta-rutinoside, rutin, carthamin, and luteolin, in the quinochalcone-type safflower line. Also, the accumulation of kaempferol-3-O-beta-rutinoside and kaempferol-3-O-beta-D-glucoside in flavonols-typed safflower line shows enhanced accumulation pattern after methyl jasmonate treatment. Other flavonols, such as kaempferol, dihydrokaempferol and quercetin-3-O-beta-D-glucoside, in flavonols-typed safflower line present downregulation in respons to the methyl jasmonate stimulus
up