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Literature summary extracted from
- Molecular characterization of flavanone 3-hydroxylase gene and flavonoid accumulation in two chemotyped safflower lines in response to methyl jasmonate stimulation (2016), BMC Plant Biol., 16, 132 .
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 1.14.11.9 | gene CtF3H, DNA and amino acid sequence determination and analysis, phylogenic analysis, quantitative real-time PCR enzyme expression analysis, functional recombinant expression of MBP-tagged enzyme in Escherichia coli BL21(DE3)pLysS from vector pMAL-C5x leading to production of dihydrokaempferol when naringenin is available the substrate. Recombinant expression of GFP-tagged enzyme in onion cells via transfromation of Agrobacterium tumefaciens strain GV3101, where it is localized both in the nucleus and in the cytosol | Carthamus tinctorius |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 1.14.11.9 | cytosol | - |
Carthamus tinctorius | 5829 | - |
| 1.14.11.9 | additional information | subcellular localization study | Carthamus tinctorius | - |
- |
| 1.14.11.9 | nucleus | - |
Carthamus tinctorius | 5634 | - |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.14.11.9 | Fe2+ | dependent on, required for catalysis, the ferrous iron binding site HxDxnH is formed by His78, His121, His211, Asp213, His221, Asp 223, His265, His267, and His279 | Carthamus tinctorius |  |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.14.11.9 | a (2S)-flavan-4-one + 2-oxoglutarate + O2 | Carthamus tinctorius | - |
a (2R,3R)-dihydroflavonol + succinate + CO2 | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.14.11.9 | Carthamus tinctorius | M9MTF3 | a quinochalcone-type safflower line with orange-yellow flowers and a flavonol-type safflower line with white flowers, i.e. ZHH0119 line and XHH007 line, respectively | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.14.11.9 | recombinant MBP-tagged enzyme from Escherichia coli BL21(DE3)pLysS by amylose affinity chromatography | Carthamus tinctorius |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 1.14.11.9 | flower | the transcriptome expression of CtF3H shows a diametrically opposed expression pattern in a quinochalcone-type safflower line (with orange-yellow flowers) and a flavonol-type safflower line (with white flowers) under external stimulation by methyl jasmonate (MeJA), which has been identified as an elicitor of flavonoid metabolites. High expression of CtF3H in quinochalcone-type safflower line | Carthamus tinctorius | - |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.14.11.9 | a (2S)-flavan-4-one + 2-oxoglutarate + O2 | - |
Carthamus tinctorius | a (2R,3R)-dihydroflavonol + succinate + CO2 | - |
? | |
| 1.14.11.9 | a (2S)-flavan-4-one + 2-oxoglutarate + O2 | the 2-oxoglutarate binding site RxS is formed by Arg289 and Ser291 | Carthamus tinctorius | a (2R,3R)-dihydroflavonol + succinate + CO2 | - |
? | |
| 1.14.11.9 | additional information | no activity with kaempferol, dihydrokaempferol, quercetin, and dihydroquercetin | Carthamus tinctorius | ? | - |
? | |
| 1.14.11.9 | naringenin + 2-oxoglutarate + O2 | - |
Carthamus tinctorius | dihydrokaempferol + succinate + CO2 | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 1.14.11.9 | ? | x * 40720, sequence calculation, x * 40000, recombinant detagged enzyme, SDS-PAGE | Carthamus tinctorius |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 1.14.11.9 | CtF3H | - |
Carthamus tinctorius |
| 1.14.11.9 | F3H | - |
Carthamus tinctorius |
| 1.14.11.9 | flavanone 3-hydroxylase | - |
Carthamus tinctorius |
pI Value
| EC Number | Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|---|
| 1.14.11.9 | Carthamus tinctorius | sequence calculation | - |
5.57 |
Expression
| EC Number | Organism | Comment | Expression |
|---|---|---|---|
| 1.14.11.9 | Carthamus tinctorius | the enzyme flavanone 3-hydroxylase is induced by methyl jasmonate. Further metabolite analysis shows the increasing tendency of quinochalcones and flavonols, such as hydroxysafflor yellow A, kaempferol-3-O-beta-D-glucoside, kaempferol-3-O-beta-rutinoside, rutin, carthamin, and luteolin, in the quinochalcone-type safflower line. Also, the accumulation of kaempferol-3-O-beta-rutinoside and kaempferol-3-O-beta-D-glucoside in flavonols-typed safflower line shows enhanced accumulation pattern after methyl jasmonate treatment. Other flavonols, such as kaempferol, dihydrokaempferol and quercetin-3-O-beta-D-glucoside, in flavonols-typed safflower line present downregulation in respons to the methyl jasmonate stimulus | up |
General Information
| EC Number | General Information | Comment | Organism |
|---|---|---|---|
| 1.14.11.9 | evolution | the enzyme belongs to the Fe2+/2oxoglutarate-dependent dioxygenase superfamily | Carthamus tinctorius |
| 1.14.11.9 | physiological function | flavanone 3-hydroxylase (F3H) is one of the nuclear enzymes acting at the bifurcation of the flavonoid biosynthetic pathway, initiating catalysis of the 3-hydroxylation of (2S)-flavanones, such as naringenin to dihydroflavonols | Carthamus tinctorius |
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Release 2023.1 (January 2023)
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