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Literature summary extracted from

  • Jiang, X.; Liu, H.; Niu, Y.; Qi, F.; Zhang, M.; Huang, J.
    Functional identification and regulatory analysis of DELTA6-fatty acid desaturase from the oleaginous fungus Mucor sp. EIM-10 (2017), Biotechnol. Lett., 39, 453-461 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.19.3 gene Md6, DNA and amino acid sequence determination and analysis, Md6 promoter analysis in the 5'-upstream sequence, gene Md6 contains several eukaryotic fundamental transcription regulatory elements, sequence comparisons, functional recombinant expression in Saccharomyces cerevisiae strain INVSc1, the enzyme expression renders the yeast cells capable of converting linolenic acid to gamma-linolenic acid. The activity of DELTA6-desaturase increases by 2.8fold and 2.5fold when the mutant eMd6 sequence is placed upstream of -434 with forward or reverse orientations, respectively. The wild-type promoter of Md6 from Mucor sp. is a very strong promoter for DELTA6-desaturase and the sequence between -919 to -784 bp is an enhancer for DELTA6-desaturase activity Mucor sp.

Protein Variants

EC Number Protein Variants Comment Organism
1.14.19.3 additional information generation of a series deletion analysis of the promoter suggesting that the sequence between -919 to -784 bp (relative to start site), termed eMd6, is the key factor for high activity of DELTA6-desaturase. Deletion of sequence between -919 and -784 bp (pYD6784-Md6), that contains three TATA boxes, a CREB1 site, and a potential promoter region, significantly reduces DELTA6-desaturase activity to 77% that of pYD6919-Md6. Further deletion of sequence from -644 to -434 bp (pYD6434-Md6), -434 to -121 (pYD6121-Md6) significantly reduces Md6 promoter activity to less than 40 and 13% that of the predecessor Mucor sp.

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.14.19.3 membrane the enzyme contains three presumptive transmembrane regions located at regions of 194-216, 249-266 and 360-382 bp Mucor sp. 16020
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.19.3 linoleoyl-CoA + 2 ferrocytochrome b5 + O2 + 2 H+ Mucor sp.
-
gamma-linolenoyl-CoA + 2 ferricytochrome b5 + 2 H2O
-
?
1.14.19.3 linoleoyl-CoA + 2 ferrocytochrome b5 + O2 + 2 H+ Mucor sp. EIM-10
-
gamma-linolenoyl-CoA + 2 ferricytochrome b5 + 2 H2O
-
?

Organism

EC Number Organism UniProt Comment Textmining
1.14.19.3 Mucor sp. B3GQC8
-
-
1.14.19.3 Mucor sp. EIM-10 B3GQC8
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.19.3 linoleoyl-CoA + 2 ferrocytochrome b5 + O2 + 2 H+
-
Mucor sp. gamma-linolenoyl-CoA + 2 ferricytochrome b5 + 2 H2O
-
?
1.14.19.3 linoleoyl-CoA + 2 ferrocytochrome b5 + O2 + 2 H+
-
Mucor sp. EIM-10 gamma-linolenoyl-CoA + 2 ferricytochrome b5 + 2 H2O
-
?

Synonyms

EC Number Synonyms Comment Organism
1.14.19.3 DELTA6-fatty acid desaturase
-
Mucor sp.
1.14.19.3 Md6
-
Mucor sp.

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.19.3 cytochrome b5
-
Mucor sp.