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Literature summary extracted from
- Construction of a highly thermostable 1,3-1,4-beta-glucanase by combinational mutagenesis and its potential application in the brewing industry (2017), Biotechnol. Lett., 39, 113-122 .
Application
| EC Number | Application | Comment | Organism |
|---|---|---|---|
| 3.2.1.73 | food industry | the addition of mutant K20S/N31c/S40E/S43E/E46P/P102C/K117S/N125C/K165S/T187C/H205P in congress mashing decreases the filtration time and viscosity by 21.3 and 9.6 %, respectively | Bacillus tequilensis |
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 3.2.1.39 | expressed in Escherichia coli BL21(DE3) cells | Bacillus tequilensis |
| 3.2.1.73 | expression in Escherichia coli | Bacillus tequilensis |
Protein Variants
| EC Number | Protein Variants | Comment | Organism |
|---|---|---|---|
| 3.2.1.39 | K20S/N31C/S40E/S43E/E46P/P102C/K117S/N125C/K165S/T187C/H205P | the mutant shows 63% increased catalytic activity and stability compared to the wild type enzyme. Half-life values at 60°C and 70°C are 152 and 99 min, respectively | Bacillus tequilensis |
| 3.2.1.73 | K20S/N31C/S40E/S43E/E46P/P102C/K117S/N125C/K165S/T187C/H205P | mutant with increased catalytic activity and thermostability | Bacillus tequilensis |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.2.1.39 | 2-mercaptoethanol | 41% residual activity at 10 mM | Bacillus tequilensis |  |
| 3.2.1.39 | Ca2+ | 84% residual activity at 10 mM | Bacillus tequilensis | |
| 3.2.1.39 | Co2+ | 94% residual activity at 10 mM | Bacillus tequilensis | |
| 3.2.1.39 | Cu2+ | 86% residual activity at 10 mM | Bacillus tequilensis | |
| 3.2.1.39 | dithiothreitol | 35% residual activity at 10 mM | Bacillus tequilensis | |
| 3.2.1.39 | EDTA | 31% residual activity at 10 mM | Bacillus tequilensis | |
| 3.2.1.39 | Fe2+ | 8.4% residual activity at 10 mM | Bacillus tequilensis | |
| 3.2.1.39 | Fe3+ | 6.5% residual activity at 10 mM | Bacillus tequilensis | |
| 3.2.1.39 | Li+ | 51% residual activity at 10 mM | Bacillus tequilensis | |
| 3.2.1.39 | Mg2+ | 50% residual activity at 10 mM | Bacillus tequilensis | |
| 3.2.1.39 | Mn2+ | 55% residual activity at 10 mM | Bacillus tequilensis | |
| 3.2.1.39 | Zn2+ | 35% residual activity at 10 mM | Bacillus tequilensis | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.2.1.39 | 0.27 | - |
Barley beta-glucan | mutant enzyme K20S/N31C/S40E/S43E/E46P/P102C/K117S/N125C/K165S/T187C/H205P, at pH 6.5 and 40°C | Bacillus tequilensis | |
| 3.2.1.39 | 0.3 | - |
Barley beta-glucan | wild type enzyme, at pH 6.5 and 40°C | Bacillus tequilensis | |
| 3.2.1.73 | 0.27 | - |
azo-barley glucan | mutant K20S/N31C/S40E/S43E/E46P/P102C/K117S/N125C/K165S/T187C/H205P, pH 6.5, 40°C | Bacillus tequilensis | |
| 3.2.1.73 | 0.3 | - |
azo-barley glucan | wild-type, pH 6.5, 40°C | Bacillus tequilensis |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.2.1.39 | additional information | not influenced by K+, Na+, or NH4+ | Bacillus tequilensis |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.2.1.39 | Bacillus tequilensis | - |
- |
- |
| 3.2.1.39 | Bacillus tequilensis CGX 5-2 | - |
- |
- |
| 3.2.1.73 | Bacillus tequilensis | A0A1W6AKT2 | sequence of mutant K20S/N31C/S40E/S43E/E46P/P102C/K117S/N125C/K165S/T187C/H205P | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.2.1.39 | Ni-NTA column chromatography | Bacillus tequilensis |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.73 | 2490 | - |
wild-type, pH 6.5, 40°C | Bacillus tequilensis |
| 3.2.1.73 | 4274 | - |
mutant K20S/N31C/CS40E/S43E/E46P/P102C/K117S/N125C/K165S/T187C/H205P, pH 6.5, 40°C | Bacillus tequilensis |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.2.1.39 | barley beta-glucan + H2O | - |
Bacillus tequilensis | ? | - |
? | |
| 3.2.1.39 | barley beta-glucan + H2O | - |
Bacillus tequilensis CGX 5-2 | ? | - |
? | |
| 3.2.1.73 | azo-barley glucan + H2O | - |
Bacillus tequilensis | ? | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.2.1.39 | Bgl | - |
Bacillus tequilensis |
| 3.2.1.39 | endo-beta-1,3-glucanase | - |
Bacillus tequilensis |
| 3.2.1.73 | BglTO | - |
Bacillus tequilensis |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.39 | 45 | - |
- |
Bacillus tequilensis |
| 3.2.1.73 | 45 | - |
wild-type | Bacillus tequilensis |
| 3.2.1.73 | 70 | - |
mutant K20S/N31C/S40E/S43E/E46P/P102C/K117S/N125C/K165S/T187C/H205P | Bacillus tequilensis |
Temperature Range [°C]
| EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.39 | 35 | 55 | more than 70% activity between 35 and 55°C | Bacillus tequilensis |
| 3.2.1.73 | 35 | 55 | wild-type, more than 80% of maximum activity | Bacillus tequilensis |
| 3.2.1.73 | 35 | 70 | mutant K20S/N31C/S40E/S43E/E46P/P102C/K117S/N125C/K165S/T187C/H205P, more than 80% of maximum activity | Bacillus tequilensis |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.39 | 60 | 70 | half-life values at 60°C and 70°C are 32.5 and 11 min, respectively. The enzyme is almost fully inactivated after incubation at 60°C for 140 min | Bacillus tequilensis |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.2.1.39 | 137 | - |
Barley beta-glucan | wild type enzyme, at pH 6.5 and 40°C | Bacillus tequilensis | |
| 3.2.1.39 | 189 | - |
Barley beta-glucan | mutant enzyme K20S/N31C/S40E/S43E/E46P/P102C/K117S/N125C/K165S/T187C/H205P, at pH 6.5 and 40°C | Bacillus tequilensis | |
| 3.2.1.73 | 137 | - |
azo-barley glucan | wild-type, pH 6.5, 40°C | Bacillus tequilensis | |
| 3.2.1.73 | 189 | - |
azo-barley glucan | mutant K20S/N31C/S40E/S43E/E46P/P102C/K117S/N125C/K165S/T187C/H205P, pH 6.5, 40°C | Bacillus tequilensis |
kcat/KM [mM/s]
| EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.2.1.39 | 462 | - |
Barley beta-glucan | wild type enzyme, at pH 6.5 and 40°C | Bacillus tequilensis | |
| 3.2.1.39 | 691 | - |
Barley beta-glucan | mutant enzyme K20S/N31C/S40E/S43E/E46P/P102C/K117S/N125C/K165S/T187C/H205P, at pH 6.5 and 40°C | Bacillus tequilensis | |
| 3.2.1.73 | 462 | - |
azo-barley glucan | wild-type, pH 6.5, 40°C | Bacillus tequilensis | |
| 3.2.1.73 | 691 | - |
azo-barley glucan | mutant K20S/N31C/S40E/S43E/E46P/P102C/K117S/N125C/K165S/T187C/H205P, pH 6.5, 40°C | Bacillus tequilensis |
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