Cloning, expression and stress-respondent transcription of long-chain acyl-coenzyme A synthetase cDNA gene of Nannochloropsis gaditana and its involvement in the biosynthesis of eicosapentaenoic and decosahexaenoic acids
Zheng, M.; Huang, Y.; Zheng, L.; Sun, Z.; Wang, L.; Biotechnol. Lett. 36, 141-145 (2014)
No PubMed abstract available
Data extracted from this reference:
Cloned(Commentary)
EC Number
Cloned (Commentary)
Organism
6.2.1.3
expressed in Saccharomyces cerevisiae
Nannochloropsis gaditana
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
6.2.1.3
ATP + a long-chain fatty acid + CoA
Nannochloropsis gaditana
the enzyme can elongate and desaturate fatty acids up to 22:6
AMP + diphosphate + a long-chain acyl-CoA
-
-
?
Organism
EC Number
Organism
UniProt
Commentary
Textmining
6.2.1.3
Nannochloropsis gaditana
S5PTC7
-
-
6.2.1.3
Nannochloropsis gaditana HH-1
S5PTC7
-
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
6.2.1.3
ATP + a long-chain fatty acid + CoA
the enzyme can elongate and desaturate fatty acids up to 22:6
744572
Nannochloropsis gaditana
AMP + diphosphate + a long-chain acyl-CoA
-
-
-
?
6.2.1.3
ATP + arachidonate + CoA
-
744572
Nannochloropsis gaditana
AMP + diphosphate + arachidonoyl-CoA
-
-
-
?
6.2.1.3
ATP + arachidonate + CoA
-
744572
Nannochloropsis gaditana HH-1
AMP + diphosphate + arachidonoyl-CoA
-
-
-
?
6.2.1.3
ATP + dihomo-gamma-linolenate + CoA
-
744572
Nannochloropsis gaditana
AMP + diphosphate + dihomo-gamma-linolenoyl-CoA
-
-
-
?
6.2.1.3
ATP + docosahexaenoate + CoA
second best substrate
744572
Nannochloropsis gaditana
AMP + diphosphate + docosahexaenoyl-CoA
-
-
-
?
6.2.1.3
ATP + eicosadienoate + CoA
-
744572
Nannochloropsis gaditana
AMP + diphosphate + eicosadienoyl-CoA
-
-
-
?
6.2.1.3
ATP + eicosapentaenoate + CoA
best substrate
744572
Nannochloropsis gaditana
AMP + diphosphate + eicosapentaenoyl-CoA
-
-
-
?
6.2.1.3
ATP + eicosapentaenoate + CoA
best substrate
744572
Nannochloropsis gaditana HH-1
AMP + diphosphate + eicosapentaenoyl-CoA
-
-
-
?
6.2.1.3
ATP + oleate + CoA
-
744572
Nannochloropsis gaditana
AMP + diphosphate + oleoyl-CoA
-
-
-
?
6.2.1.3
ATP + oleate + CoA
-
744572
Nannochloropsis gaditana HH-1
AMP + diphosphate + oleoyl-CoA
-
-
-
?
6.2.1.3
ATP + palmitate + CoA
-
744572
Nannochloropsis gaditana
AMP + diphosphate + palmitoyl-CoA
-
-
-
?
6.2.1.3
ATP + palmitate + CoA
-
744572
Nannochloropsis gaditana HH-1
AMP + diphosphate + palmitoyl-CoA
-
-
-
?
6.2.1.3
ATP + palmitoleate + CoA
-
744572
Nannochloropsis gaditana
AMP + diphosphate + palmitoleoyl-CoA
-
-
-
?
6.2.1.3
ATP + stearate + CoA
-
744572
Nannochloropsis gaditana
AMP + diphosphate + stearoyl-CoA
-
-
-
?
6.2.1.3
additional information
low activity with caprate, laurate, myristate, eicosanoate, and docosanoate
744572
Nannochloropsis gaditana
?
-
-
-
?
6.2.1.3
additional information
low activity with caprate, laurate, myristate, eicosanoate, and docosanoate
744572
Nannochloropsis gaditana HH-1
?
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
6.2.1.3
?
x * 71000, calculated from amino acid sequence
Nannochloropsis gaditana
Synonyms
EC Number
Synonyms
Commentary
Organism
6.2.1.3
LACS
-
Nannochloropsis gaditana
6.2.1.3
Long-chain acyl-coenzyme A synthetase
-
Nannochloropsis gaditana
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
6.2.1.3
ATP
-
Nannochloropsis gaditana
pI Value
EC Number
Organism
Commentary
pI Value Maximum
pI Value
6.2.1.3
Nannochloropsis gaditana
calculated from amino acid sequence
-
7.8
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
6.2.1.3
expressed in Saccharomyces cerevisiae
Nannochloropsis gaditana
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
6.2.1.3
ATP
-
Nannochloropsis gaditana
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
6.2.1.3
ATP + a long-chain fatty acid + CoA
Nannochloropsis gaditana
the enzyme can elongate and desaturate fatty acids up to 22:6
AMP + diphosphate + a long-chain acyl-CoA
-
-
?
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
6.2.1.3
ATP + a long-chain fatty acid + CoA
the enzyme can elongate and desaturate fatty acids up to 22:6
744572
Nannochloropsis gaditana
AMP + diphosphate + a long-chain acyl-CoA
-
-
-
?
6.2.1.3
ATP + arachidonate + CoA
-
744572
Nannochloropsis gaditana
AMP + diphosphate + arachidonoyl-CoA
-
-
-
?
6.2.1.3
ATP + arachidonate + CoA
-
744572
Nannochloropsis gaditana HH-1
AMP + diphosphate + arachidonoyl-CoA
-
-
-
?
6.2.1.3
ATP + dihomo-gamma-linolenate + CoA
-
744572
Nannochloropsis gaditana
AMP + diphosphate + dihomo-gamma-linolenoyl-CoA
-
-
-
?
6.2.1.3
ATP + docosahexaenoate + CoA
second best substrate
744572
Nannochloropsis gaditana
AMP + diphosphate + docosahexaenoyl-CoA
-
-
-
?
6.2.1.3
ATP + eicosadienoate + CoA
-
744572
Nannochloropsis gaditana
AMP + diphosphate + eicosadienoyl-CoA
-
-
-
?
6.2.1.3
ATP + eicosapentaenoate + CoA
best substrate
744572
Nannochloropsis gaditana
AMP + diphosphate + eicosapentaenoyl-CoA
-
-
-
?
6.2.1.3
ATP + eicosapentaenoate + CoA
best substrate
744572
Nannochloropsis gaditana HH-1
AMP + diphosphate + eicosapentaenoyl-CoA
-
-
-
?
6.2.1.3
ATP + oleate + CoA
-
744572
Nannochloropsis gaditana
AMP + diphosphate + oleoyl-CoA
-
-
-
?
6.2.1.3
ATP + oleate + CoA
-
744572
Nannochloropsis gaditana HH-1
AMP + diphosphate + oleoyl-CoA
-
-
-
?
6.2.1.3
ATP + palmitate + CoA
-
744572
Nannochloropsis gaditana
AMP + diphosphate + palmitoyl-CoA
-
-
-
?
6.2.1.3
ATP + palmitate + CoA
-
744572
Nannochloropsis gaditana HH-1
AMP + diphosphate + palmitoyl-CoA
-
-
-
?
6.2.1.3
ATP + palmitoleate + CoA
-
744572
Nannochloropsis gaditana
AMP + diphosphate + palmitoleoyl-CoA
-
-
-
?
6.2.1.3
ATP + stearate + CoA
-
744572
Nannochloropsis gaditana
AMP + diphosphate + stearoyl-CoA
-
-
-
?
6.2.1.3
additional information
low activity with caprate, laurate, myristate, eicosanoate, and docosanoate
744572
Nannochloropsis gaditana
?
-
-
-
?
6.2.1.3
additional information
low activity with caprate, laurate, myristate, eicosanoate, and docosanoate
744572
Nannochloropsis gaditana HH-1
?
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
6.2.1.3
?
x * 71000, calculated from amino acid sequence
Nannochloropsis gaditana
pI Value (protein specific)
EC Number
Organism
Commentary
pI Value Maximum
pI Value
6.2.1.3
Nannochloropsis gaditana
calculated from amino acid sequence
-
7.8
Expression
EC Number
Organism
Commentary
Expression
6.2.1.3
Nannochloropsis gaditana
enzyme transcription is up-regulated when treated by nitrogen and iron excess and at 15 and 35°C
up
Expression (protein specific)
EC Number
Organism
Commentary
Expression
6.2.1.3
Nannochloropsis gaditana
enzyme transcription is up-regulated when treated by nitrogen and iron excess and at 15 and 35°C
up