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Literature summary extracted from

  • Lee, E.; Kim, H.; Kim, D.; Kim, Y.; Nam, S.; Kim, B.; Jeon, S.
    Gene expression and characterization of thermostable glutamate decarboxylase from Pyrococcus furiosus (2013), Biotechnol. Bioprocess Eng., 18, 375-381 .
No PubMed abstract available

Application

EC Number Application Comment Organism
4.1.1.15 synthesis can be used as an industrially effective tool for the synthesis of gamma-aminobutyric acid Pyrococcus furiosus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.1.1.11 expression in Escherichia coli (DE3) Pyrococcus furiosus
4.1.1.15 expression in Escherichia coli (DE3) Pyrococcus furiosus
4.1.1.25 expression in Escherichia coli (DE3) Pyrococcus furiosus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.1.1.11 2.16
-
 L-aspartate pH 6.0, 75°C Pyrococcus furiosus
4.1.1.15 2.22
-
 L-glutamate pH 6.0, 75°C Pyrococcus furiosus
4.1.1.25 4.45
-
 L-tyrosine pH 6.0, 75°C Pyrococcus furiosus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.1.1.11 CaCl2 activity is dependent on various salts, such as CaCl2, NaCl, KCl, and NaBr, with an optimum concentration of 400 mM Pyrococcus furiosus
4.1.1.11 KCl activity is dependent on various salts, such as CaCl2, NaCl, KCl, and NaBr, with an optimum concentration of 400 mM Pyrococcus furiosus
4.1.1.11 NaBr activity is dependent on various salts, such as CaCl2, NaCl, KCl, and NaBr, with an optimum concentration of 400 mM Pyrococcus furiosus
4.1.1.11 NaCl activity is dependent on various salts, such as CaCl2, NaCl, KCl, and NaBr, with an optimum concentration of 400 mM Pyrococcus furiosus
4.1.1.15 CaCl2 activity is dependent on various salts, such as CaCl2, NaCl, KCl, and NaBr, with an optimum concentration of 400 mM Pyrococcus furiosus
4.1.1.15 KCl activity is dependent on various salts, such as CaCl2, NaCl, KCl, and NaBr, with an optimum concentration of 400 mM Pyrococcus furiosus
4.1.1.15 NaBr activity is dependent on various salts, such as CaCl2, NaCl, KCl, and NaBr, with an optimum concentration of 400 mM Pyrococcus furiosus
4.1.1.15 NaCl activity is dependent on various salts, such as CaCl2, NaCl, KCl, and NaBr, with an optimum concentration of 400 mM Pyrococcus furiosus
4.1.1.25 CaCl2 activity is dependent on various salts, such as CaCl2, NaCl, KCl, and NaBr, with an optimum concentration of 400 mM Pyrococcus furiosus
4.1.1.25 KCl activity is dependent on various salts, such as CaCl2, NaCl, KCl, and NaBr, with an optimum concentration of 400 mM Pyrococcus furiosus
4.1.1.25 NaBr activity is dependent on various salts, such as CaCl2, NaCl, KCl, and NaBr, with an optimum concentration of 400 mM Pyrococcus furiosus
4.1.1.25 NaCl activity is dependent on various salts, such as CaCl2, NaCl, KCl, and NaBr, with an optimum concentration of 400 mM Pyrococcus furiosus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.1.1.11 40000
-
 gel filtration Pyrococcus furiosus
4.1.1.11 41000
-
 SDS-PAGE Pyrococcus furiosus
4.1.1.15 40000
-
 gel filtration Pyrococcus furiosus
4.1.1.15 41000
-
 SDS-PAGE Pyrococcus furiosus
4.1.1.25 40000
-
 gel filtration Pyrococcus furiosus
4.1.1.25 41000
-
 SDS-PAGE Pyrococcus furiosus

Organism

EC Number Organism UniProt Comment Textmining
4.1.1.11 Pyrococcus furiosus Q8U1P6
-
-
4.1.1.15 Pyrococcus furiosus Q8U1P6
-
-
4.1.1.25 Pyrococcus furiosus Q8U1P6
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.1.11
-
 Pyrococcus furiosus
4.1.1.15
-
 Pyrococcus furiosus
4.1.1.25
-
 Pyrococcus furiosus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.1.11 L-aspartate the enzyme also catalyzes the decarboxylation of L-glutamate with slightly lower efficiency. No activity with D-aspartate Pyrococcus furiosus beta-alanine + CO2
-
 ?
4.1.1.15 L-glutamate the enzyme also catalyzes the decarboxylation of L-aspartate with slightly higher efficiency. No activity with D-glutamate Pyrococcus furiosus 4-aminobutanoate + CO2
-
 ?
4.1.1.25 L-Tyrosine the enzyme also catalyzes the decarboxylation of L-glutamate and L-asparate with higher efficiency. No activity with D-tyrosine Pyrococcus furiosus Tyramine + CO2
-
 ?

Subunits

EC Number Subunits Comment Organism
4.1.1.11 monomer 1 * 410000, SDS-PAGE Pyrococcus furiosus
4.1.1.15 monomer 1 * 410000, SDS-PAGE Pyrococcus furiosus
4.1.1.25 monomer 1 * 410000, SDS-PAGE Pyrococcus furiosus

Synonyms

EC Number Synonyms Comment Organism
4.1.1.11 PF1159
-
 Pyrococcus furiosus
4.1.1.15 PF1159
-
 Pyrococcus furiosus
4.1.1.15 PfGAD
-
 Pyrococcus furiosus
4.1.1.25 PF1159
-
 Pyrococcus furiosus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.1.1.11 75
-
-
 Pyrococcus furiosus
4.1.1.15 75
-
-
 Pyrococcus furiosus
4.1.1.25 75
-
-
 Pyrococcus furiosus

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
4.1.1.11 40 85 40°C: about 50% of maximal activity, 85°C: about 50% of maximal activity Pyrococcus furiosus
4.1.1.15 40 85 40°C: about 50% of maximal activity, 85°C: about 50% of maximal activity Pyrococcus furiosus
4.1.1.25 40 85 40°C: about 50% of maximal activity, 85°C: about 50% of maximal activity Pyrococcus furiosus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4.1.1.11 90
-
 half-life: 60 min Pyrococcus furiosus
4.1.1.15 90
-
 half-life: 60 min Pyrococcus furiosus
4.1.1.25 90
-
 half-life: 60 min Pyrococcus furiosus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.1.1.11 2.83
-
 L-aspartate pH 6.0, 75°C Pyrococcus furiosus
4.1.1.15 1.43
-
 L-glutamate pH 6.0, 75°C Pyrococcus furiosus
4.1.1.25 2.83
-
 L-tyrosine pH 6.0, 75°C Pyrococcus furiosus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.1.1.11 6
-
-
 Pyrococcus furiosus
4.1.1.15 6
-
-
 Pyrococcus furiosus
4.1.1.25 6
-
-
 Pyrococcus furiosus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
4.1.1.11 4 7.5 pH 4.0: about 40% of maximal activity, pH 7.5: about 45% of maximal activity Pyrococcus furiosus
4.1.1.15 4 7.5 pH 4.0: about 40% of maximal activity, pH 7.5: about 45% of maximal activity Pyrococcus furiosus
4.1.1.25 4 7.5 pH 4.0: about 40% of maximal activity, pH 7.5: about 45% of maximal activity Pyrococcus furiosus

Cofactor

EC Number Cofactor Comment Organism Structure
4.1.1.11 pyridoxal 5'-phosphate the enzyme contains pyridoxal 5'-phosphate Pyrococcus furiosus
4.1.1.15 pyridoxal 5'-phosphate the enzyme contains pyridoxal 5'-phosphate Pyrococcus furiosus
4.1.1.25 pyridoxal 5'-phosphate the ennzyme contains pyridoxal 5'-phosphate Pyrococcus furiosus

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
4.1.1.11 0.76
-
 L-aspartate pH 6.0, 75°C Pyrococcus furiosus
4.1.1.15 0.64
-
 L-glutamate pH 6.0, 75°C Pyrococcus furiosus
4.1.1.25 0.37
-
 L-tyrosine pH 6.0, 75°C Pyrococcus furiosus