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Literature summary extracted from

  • Kim, J.; Shin, E.; Jeon, S.; Kim, Y.; Kim, P.; Lee, C.; Nam, S.
    Overexpression, purification, and functional characterization of the group II chaperonin from the hyperthermophilic archaeum Pyrococcus horikoshii OT3 (2009), Biotechnol. Bioprocess Eng., 14, 551-558 .
No PubMed abstract available

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.6.4.B10 the chaperonin gene PhCpn is amplified by PCR from the genomic DNA, subcloned into pET21a vector, and expressed in three Escherichia coli host cells such as BL21, Rosetta, and Codonplus (DE3)DE3. Among these host cells, Escherichia coli Rosetta shows the highest expression level of recombinant PhCpn at induction with 1 mM isopropyl beta-D-thiogalactoside Pyrococcus horikoshii

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.6.4.B10 Mg2+ with the addition of Mg2+ ion protection activity on inorganic phosphatase from thermal inactivation at 85°C and 110°C increases 2fold compared with the addition of enzyme alone Pyrococcus horikoshii

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.6.4.B10 59600
-
 SDS-PAGE Pyrococcus horikoshii

Organism

EC Number Organism UniProt Comment Textmining
3.6.4.B10 Pyrococcus horikoshii O57762
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.6.4.B10 the recombinant enzyme is purified to 91% by heat-shock treatment and anion-exchange chromatography Pyrococcus horikoshii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.6.4.B10 ATP + H2O activity of the enzyme as a molecular chaperone is examined using hyperthermophilic inorganic phosphatase from Pyrococcus horikoshii as a model substrate. The enzyme protected the inorganic phosphatase from thermal inactivation at 85°C and 110°C Pyrococcus horikoshii ADP + phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.6.4.B10 ? x * 59600, SDS-PAGE Pyrococcus horikoshii

Synonyms

EC Number Synonyms Comment Organism
3.6.4.B10 More ordered locus name: PH0017 Pyrococcus horikoshii
3.6.4.B10 PhCPN
-
 Pyrococcus horikoshii

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.6.4.B10 85
-
 assay at Pyrococcus horikoshii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.6.4.B10 7.5
-
 assay at Pyrococcus horikoshii