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Literature summary extracted from

  • Dotsenko, A.; Gusakov, A.; Volkov, P.; Rozhkova, A.; Sinitsyn, A.
    N-linked glycosylation of recombinant cellobiohydrolase I (Cel7A) from Penicillium verruculosum and its effect on the enzyme activity (2016), Biotechnol. Bioeng., 113, 283-291 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.176 expressed in Penicillium canescens strain PCA10 Talaromyces verruculosus

Protein Variants

EC Number Protein Variants Comment Organism
3.2.1.176 N194A the substrate digestion rates of the mutant are notably lower relative to the wild type enzyme Talaromyces verruculosus
3.2.1.176 N388A the substrate digestion rates of the mutant are notably lower relative to the wild type enzyme Talaromyces verruculosus
3.2.1.176 N45A the mutation leads to a significant increase in the rate of Avicel and milled aspen wood hydrolysis compared to the wild type enzyme Talaromyces verruculosus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.176 cellobiose
-
 Talaromyces verruculosus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.176 cellulose + H2O Talaromyces verruculosus
-
 cellobiose + ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.176 Talaromyces verruculosus
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.2.1.176 glycoprotein the enzyme has four potential N-glycosylation sites at its catalytic module: Asn45, Asn194, Asn388, and Asn430 Talaromyces verruculosus

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.176 ammonium sulfate precipitation, Source 15Q column chromatography, and Source 15 isopropyl column chromatography Talaromyces verruculosus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.176 0.028
-
 wild type enzyme, with 4-nitrophenyl beta-D-lactoside as substrate, at pH 4.0 and 40°C Talaromyces verruculosus
3.2.1.176 0.036
-
 mutant enzyme N194A, with 4-nitrophenyl beta-D-lactoside as substrate, at pH 4.0 and 40°C Talaromyces verruculosus
3.2.1.176 0.038
-
 mutant enzyme N45A, with 4-nitrophenyl beta-D-lactoside as substrate, at pH 4.0 and 40°C Talaromyces verruculosus
3.2.1.176 0.039
-
 mutant enzyme N388A, with 4-nitrophenyl beta-D-lactoside as substrate, at pH 4.0 and 40°C Talaromyces verruculosus
3.2.1.176 0.041
-
 mutant enzyme N388A, with beta-glucan as substrate, at pH 4.0 and 40°C Talaromyces verruculosus
3.2.1.176 0.044
-
 mutant enzyme N194A, with beta-glucan as substrate, at pH 4.0 and 40°C Talaromyces verruculosus
3.2.1.176 0.047
-
 wild type enzyme, with beta-glucan as substrate, at pH 4.0 and 40°C Talaromyces verruculosus
3.2.1.176 0.117
-
 mutant enzyme N45A, with beta-glucan as substrate, at pH 4.0 and 40°C Talaromyces verruculosus
3.2.1.176 0.17
-
 mutant enzyme N194A, with Avicel as substrate, at pH 4.0 and 40°C Talaromyces verruculosus
3.2.1.176 0.19
-
 mutant enzyme N388A, with Avicel as substrate, at pH 4.0 and 40°C Talaromyces verruculosus
3.2.1.176 0.2
-
 wild type enzyme, with Avicel as substrate, at pH 4.0 and 40°C Talaromyces verruculosus
3.2.1.176 0.22
-
 mutant enzyme N45A, with Avicel as substrate, at pH 4.0 and 40°C Talaromyces verruculosus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.176 4-nitrophenyl beta-D-lactoside + H2O
-
 Talaromyces verruculosus 4-nitrophenol + beta-D-lactose
-
 ?
3.2.1.176 avicel + H2O highest activity Talaromyces verruculosus ?
-
 ?
3.2.1.176 beta-glucan + H2O
-
 Talaromyces verruculosus ?
-
 ?
3.2.1.176 cellulose + H2O
-
 Talaromyces verruculosus cellobiose + ?
-
 ?
3.2.1.176 milled aspen wood + H2O
-
 Talaromyces verruculosus ?
-
 ?
3.2.1.176 additional information no activity with carboxymethyl cellulose, 4-nitrophenyl beta-D-glucopyranoside, and 4-nitrophenyl beta-D-galactopyranoside Talaromyces verruculosus ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.176 ? x * 70000, SDS-PAGE Talaromyces verruculosus

Synonyms

EC Number Synonyms Comment Organism
3.2.1.176 Cel7A
-
 Talaromyces verruculosus
3.2.1.176 cellobiohydrolase I
-
 Talaromyces verruculosus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.176 50 65 the enzyme is stable at 50°C at least for 3 h. At 60 and 65°C, the enzyme half-life times are in the range of 40-43 min and 11-13 min, respectively Talaromyces verruculosus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.176 4
-
-
 Talaromyces verruculosus