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Literature summary extracted from

  • Lu, W.; Levy, M.; Kincaid, R.; Ellington, A.
    Directed evolution of the substrate specificity of biotin ligase (2014), Biotechnol. Bioeng., 111, 1071-1081 .
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
6.3.4.15 I11V/P85S/ M157T/K163E/I224T 17fold increase in the ability to use desthiobiotin as a substrate Escherichia coli
6.3.4.15 M157T 2-3fold increase in kcat value for desthiobiotin as a substrate Escherichia coli
6.3.4.15 Q41R/M157T/I224T/E215D/T225A 10fold increase in the ability to use desthiobiotin as a substrate Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.3.4.15 0.0017
-
 desthiobiotin wild-type, pH 8.3, 30°C Escherichia coli
6.3.4.15 0.003
-
 biotin wild-type, pH 8.3, 30°C Escherichia coli
6.3.4.15 0.0043
-
 desthiobiotin mutant M157T, pH 8.3, 30°C Escherichia coli
6.3.4.15 0.0067
-
 desthiobiotin mutant I11V/P85S/ M157T/K163E/I224T, pH 8.3, 30°C Escherichia coli
6.3.4.15 0.22
-
 ATP wild-type, pH 8.3, 30°C Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
6.3.4.15 Escherichia coli
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.3.4.15 ATP + biotin + CGGGSGGGSGLNDIFEAQKIEWH
-
 Escherichia coli AMP + diphosphate + biotinylated CGGGSGGGSGLNDIFEAQKIEWH
-
 ?
6.3.4.15 ATP + desthiobiotin + CGGGSGGGSGLNDIFEAQKIEWH
-
 Escherichia coli AMP + diphosphate + desthiobiotinylated CGGGSGGGSGLNDIFEAQKIEWH
-
 ?

Synonyms

EC Number Synonyms Comment Organism
6.3.4.15 BirA
-
 Escherichia coli
6.3.4.15 BPL
-
 Escherichia coli

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
6.3.4.15 0.29
-
 desthiobiotin wild-type, pH 8.3, 30°C Escherichia coli
6.3.4.15 0.3
-
 biotin wild-type, pH 8.3, 30°C Escherichia coli
6.3.4.15 0.48
-
 ATP wild-type, pH 8.3, 30°C Escherichia coli
6.3.4.15 0.53
-
 desthiobiotin mutant M157T, pH 8.3, 30°C Escherichia coli
6.3.4.15 0.73
-
 desthiobiotin mutant I11V/P85S/ M157T/K163E/I224T, pH 8.3, 30°C Escherichia coli

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
6.3.4.15 100
-
 desthiobiotin mutant I11V/P85S/ M157T/K163E/I224T, pH 8.3, 30°C Escherichia coli
6.3.4.15 120
-
 desthiobiotin mutant M157T, pH 8.3, 30°C Escherichia coli
6.3.4.15 170
-
 desthiobiotin wild-type, pH 8.3, 30°C Escherichia coli
6.3.4.15 1050
-
 biotin wild-type, pH 8.3, 30°C Escherichia coli