EC Number | Cloned (Comment) | Organism |
---|---|---|
3.1.1.73 | expression in Escherichia coli and Pichia pastoris | Pleurotus sapidus |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
3.1.1.73 | additional information | expression in Pichia pastoris with an N-terminal alpha-mating factor pre-pro sequence and under the control of a methanol inducible alcohol oxidase 1 promotor | Pleurotus sapidus |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.1.1.73 | 0.227 | - |
beta-D-xylopyranosyl-(1->2)-5-O-trans-feruloyl-L-arabinofuranose | pH 5.0, 37°C | Pleurotus sapidus | |
3.1.1.73 | 0.427 | - |
methyl ferulate | pH 5.0, 37°C | Pleurotus sapidus |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.1.1.73 | Pleurotus sapidus | D5GRP3 | - |
- |
EC Number | Posttranslational Modification | Comment | Organism |
---|---|---|---|
3.1.1.73 | glycoprotein | molecular mass of deglycosylated protein is around 60000 Da on SDS-PAGE and 59400, calculated. Molecular mass of glycosylated protein is around 70000 Da | Pleurotus sapidus |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.1.1.73 | recombinant protein | Pleurotus sapidus |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
3.1.1.73 | 1027 | - |
substrate methyl ferulate, pH 5.0, 37°C | Pleurotus sapidus |
3.1.1.73 | 1681 | - |
substrate beta-D-xylopyranosyl-(1->2)-5-O-trans-feruloyl-L-arabinofuranose, pH 5.0, 37°C | Pleurotus sapidus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.1.1.73 | 5-O-(trans-feruloyl)-L-arabinofuranose + H2O | 83% of the activity with beta-D-xylopyranosyl-(1->2)-5-O-trans-feruloyl-L-arabinofuranose | Pleurotus sapidus | ferulic acid + L-arabinose | - |
? | |
3.1.1.73 | alpha-L-galactopyranosyl-(1->2)-beta-D-xylopyranosyl-(1->2)-5-O-trans-feruloyl-L-arabinofuranose + H2O | 48.4% of the activity with beta-D-xylopyranosyl-(1->2)-5-O-trans-feruloyl-L-arabinofuranose | Pleurotus sapidus | alpha-L-galactopyranosyl-(1->2)-beta-D-xylopyranosyl-(1->2)-L-arabinofuranose + ferulate | - |
? | |
3.1.1.73 | beta-D-xylopyranosyl-(1->2)-5-O-trans-feruloyl-L-arabinofuranose + H2O | - |
Pleurotus sapidus | beta-D-xylopyranosyl-(1->2)-L-arabinofuranose + ferulate | - |
? | |
3.1.1.73 | destarched wheat bran + H2O | - |
Pleurotus sapidus | ferulate + ? | - |
? | |
3.1.1.73 | ethyl ferulate + H2O | 61.1% of the activity with beta-D-xylopyranosyl-(1->2)-5-O-trans-feruloyl-L-arabinofuranose | Pleurotus sapidus | ferulate + ethanol | - |
? | |
3.1.1.73 | methyl ferulate + H2O | 62.6% of the activity with beta-D-xylopyranosyl-(1->2)-5-O-trans-feruloyl-L-arabinofuranose | Pleurotus sapidus | ferulate + methanol | - |
? | |
3.1.1.73 | methyl sinapinate + H2O | 22.4% of the activity with beta-D-xylopyranosyl-(1->2)-5-O-trans-feruloyl-L-arabinofuranose | Pleurotus sapidus | sinapinate + methanol | - |
? | |
3.1.1.73 | additional information | enzyme catalyzes transesterification of methyl ferulate using D-glucose, D-fructose, and D-galactose and D-sucrose or D-maltose | Pleurotus sapidus | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.1.1.73 | ? | x * 70000, SDS-PAGE, recombinant protein, x * 60000, SDS-PAGE of Endo H treated protein | Pleurotus sapidus |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.1.1.73 | Est1 | - |
Pleurotus sapidus |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.1.1.73 | 50 | - |
- |
Pleurotus sapidus |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.1.1.73 | 1.2 | - |
methyl ferulate | pH 5.0, 37°C | Pleurotus sapidus | |
3.1.1.73 | 1.96 | - |
beta-D-xylopyranosyl-(1->2)-5-O-trans-feruloyl-L-arabinofuranose | pH 5.0, 37°C | Pleurotus sapidus |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.1.1.73 | 5 | - |
recombinant protein | Pleurotus sapidus |
3.1.1.73 | 6 | - |
native protein | Pleurotus sapidus |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
3.1.1.73 | 3 | 8 | no activity below/above | Pleurotus sapidus |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.1.1.73 | 2.81 | - |
methyl ferulate | pH 5.0, 37°C | Pleurotus sapidus | |
3.1.1.73 | 8.64 | - |
beta-D-xylopyranosyl-(1->2)-5-O-trans-feruloyl-L-arabinofuranose | pH 5.0, 37°C | Pleurotus sapidus |