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Literature summary extracted from

  • Kelle, S.; Nieter, A.; Krings, U.; Zelena, K.; Linke, D.; Berger, R.
    Heterologous production of a feruloyl esterase from Pleurotus sapidus synthesizing feruloyl-saccharide esters (2016), Biotechnol. Appl. Biochem., 63, 852-862 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.1.73 expression in Escherichia coli and Pichia pastoris Pleurotus sapidus

Protein Variants

EC Number Protein Variants Comment Organism
3.1.1.73 additional information expression in Pichia pastoris with an N-terminal alpha-mating factor pre-pro sequence and under the control of a methanol inducible alcohol oxidase 1 promotor Pleurotus sapidus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.1.73 0.227
-
beta-D-xylopyranosyl-(1->2)-5-O-trans-feruloyl-L-arabinofuranose pH 5.0, 37°C Pleurotus sapidus
3.1.1.73 0.427
-
methyl ferulate pH 5.0, 37°C Pleurotus sapidus

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.73 Pleurotus sapidus D5GRP3
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.1.1.73 glycoprotein molecular mass of deglycosylated protein is around 60000 Da on SDS-PAGE and 59400, calculated. Molecular mass of glycosylated protein is around 70000 Da Pleurotus sapidus

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.73 recombinant protein Pleurotus sapidus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.1.73 1027
-
substrate methyl ferulate, pH 5.0, 37°C Pleurotus sapidus
3.1.1.73 1681
-
substrate beta-D-xylopyranosyl-(1->2)-5-O-trans-feruloyl-L-arabinofuranose, pH 5.0, 37°C Pleurotus sapidus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.73 5-O-(trans-feruloyl)-L-arabinofuranose + H2O 83% of the activity with beta-D-xylopyranosyl-(1->2)-5-O-trans-feruloyl-L-arabinofuranose Pleurotus sapidus ferulic acid + L-arabinose
-
?
3.1.1.73 alpha-L-galactopyranosyl-(1->2)-beta-D-xylopyranosyl-(1->2)-5-O-trans-feruloyl-L-arabinofuranose + H2O 48.4% of the activity with beta-D-xylopyranosyl-(1->2)-5-O-trans-feruloyl-L-arabinofuranose Pleurotus sapidus alpha-L-galactopyranosyl-(1->2)-beta-D-xylopyranosyl-(1->2)-L-arabinofuranose + ferulate
-
?
3.1.1.73 beta-D-xylopyranosyl-(1->2)-5-O-trans-feruloyl-L-arabinofuranose + H2O
-
Pleurotus sapidus beta-D-xylopyranosyl-(1->2)-L-arabinofuranose + ferulate
-
?
3.1.1.73 destarched wheat bran + H2O
-
Pleurotus sapidus ferulate + ?
-
?
3.1.1.73 ethyl ferulate + H2O 61.1% of the activity with beta-D-xylopyranosyl-(1->2)-5-O-trans-feruloyl-L-arabinofuranose Pleurotus sapidus ferulate + ethanol
-
?
3.1.1.73 methyl ferulate + H2O 62.6% of the activity with beta-D-xylopyranosyl-(1->2)-5-O-trans-feruloyl-L-arabinofuranose Pleurotus sapidus ferulate + methanol
-
?
3.1.1.73 methyl sinapinate + H2O 22.4% of the activity with beta-D-xylopyranosyl-(1->2)-5-O-trans-feruloyl-L-arabinofuranose Pleurotus sapidus sinapinate + methanol
-
?
3.1.1.73 additional information enzyme catalyzes transesterification of methyl ferulate using D-glucose, D-fructose, and D-galactose and D-sucrose or D-maltose Pleurotus sapidus ?
-
?

Subunits

EC Number Subunits Comment Organism
3.1.1.73 ? x * 70000, SDS-PAGE, recombinant protein, x * 60000, SDS-PAGE of Endo H treated protein Pleurotus sapidus

Synonyms

EC Number Synonyms Comment Organism
3.1.1.73 Est1
-
Pleurotus sapidus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.1.73 50
-
-
Pleurotus sapidus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.1.1.73 1.2
-
methyl ferulate pH 5.0, 37°C Pleurotus sapidus
3.1.1.73 1.96
-
beta-D-xylopyranosyl-(1->2)-5-O-trans-feruloyl-L-arabinofuranose pH 5.0, 37°C Pleurotus sapidus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.73 5
-
recombinant protein Pleurotus sapidus
3.1.1.73 6
-
native protein Pleurotus sapidus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.1.1.73 3 8 no activity below/above Pleurotus sapidus

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.1.1.73 2.81
-
methyl ferulate pH 5.0, 37°C Pleurotus sapidus
3.1.1.73 8.64
-
beta-D-xylopyranosyl-(1->2)-5-O-trans-feruloyl-L-arabinofuranose pH 5.0, 37°C Pleurotus sapidus