BRENDA - Enzyme Database

Integrating an algal beta-carotene hydroxylase gene into a designed carotenoid-biosynthesis pathway increases carotenoid production in yeast

Chang, J.; Thia, C.; Lin, H.; Liu, H.; Ho, F.; Wu, J.; Shih, M.; Li, W.; Huang, C.; Biores. Technol. 184, 2-8 (2015)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
1.14.15.24
expressed in Kluyveromyces marxianus strain KY3
Chlamydomonas reinhardtii
1.14.15.24
expressed in Kluyveromyces marxianus strain KY3
Chromochloris zofingiensis
1.14.15.24
expressed in Kluyveromyces marxianus strain KY3
Haematococcus lacustris
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.14.15.24
beta-carotene + reduced ferredoxin [iron-sulfur] cluster + H+ + O2
Chlamydomonas reinhardtii
-
zeaxanthin + oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
?
1.14.15.24
beta-carotene + reduced ferredoxin [iron-sulfur] cluster + H+ + O2
Haematococcus lacustris
-
zeaxanthin + oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
?
1.14.15.24
beta-carotene + reduced ferredoxin [iron-sulfur] cluster + H+ + O2
Chromochloris zofingiensis
-
zeaxanthin + oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.14.15.24
Chlamydomonas reinhardtii
-
-
-
1.14.15.24
Chromochloris zofingiensis
-
-
-
1.14.15.24
Haematococcus lacustris
-
-
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.14.15.24
beta-carotene + reduced ferredoxin [iron-sulfur] cluster + H+ + O2
-
744519
Chlamydomonas reinhardtii
zeaxanthin + oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
1.14.15.24
beta-carotene + reduced ferredoxin [iron-sulfur] cluster + H+ + O2
-
744519
Haematococcus lacustris
zeaxanthin + oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
1.14.15.24
beta-carotene + reduced ferredoxin [iron-sulfur] cluster + H+ + O2
-
744519
Chromochloris zofingiensis
zeaxanthin + oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
1.14.15.24
expressed in Kluyveromyces marxianus strain KY3
Chlamydomonas reinhardtii
1.14.15.24
expressed in Kluyveromyces marxianus strain KY3
Chromochloris zofingiensis
1.14.15.24
expressed in Kluyveromyces marxianus strain KY3
Haematococcus lacustris
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.14.15.24
beta-carotene + reduced ferredoxin [iron-sulfur] cluster + H+ + O2
Chlamydomonas reinhardtii
-
zeaxanthin + oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
?
1.14.15.24
beta-carotene + reduced ferredoxin [iron-sulfur] cluster + H+ + O2
Haematococcus lacustris
-
zeaxanthin + oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
?
1.14.15.24
beta-carotene + reduced ferredoxin [iron-sulfur] cluster + H+ + O2
Chromochloris zofingiensis
-
zeaxanthin + oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
?
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.14.15.24
beta-carotene + reduced ferredoxin [iron-sulfur] cluster + H+ + O2
-
744519
Chlamydomonas reinhardtii
zeaxanthin + oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
1.14.15.24
beta-carotene + reduced ferredoxin [iron-sulfur] cluster + H+ + O2
-
744519
Haematococcus lacustris
zeaxanthin + oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
1.14.15.24
beta-carotene + reduced ferredoxin [iron-sulfur] cluster + H+ + O2
-
744519
Chromochloris zofingiensis
zeaxanthin + oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?