Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Rydzik, A.M.; Brem, J.; Struwe, W.B.; Kochan, G.T.; Benesch, J.L.; Schofield, C.J.
    Ejection of structural zinc leads to inhibition of gamma-butyrobetaine hydroxylase (2014), Bioorg. Med. Chem. Lett., 24, 4954-4957 .
    View publication on PubMed

Application

EC Number Application Comment Organism
1.14.11.1 medicine inhibition of BBOX is reported to aid in recovery after acute stroke Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.14.11.1 benzeneselenenic acid
-
 Homo sapiens
1.14.11.1 benzeneselenenyl bromide
-
 Homo sapiens
1.14.11.1 benzeneselenenyl chloride
-
 Homo sapiens
1.14.11.1 benzeneseleninic acid
-
 Homo sapiens
1.14.11.1 diphenyldiselane
-
 Homo sapiens
1.14.11.1 ebselen a relatively potent BBOX inhibitor and a Zn(II) ejector Homo sapiens
1.14.11.1 N-oxalylglycine a 2-oxoglutarate mimic Homo sapiens
1.14.11.1 N-[(1-chloro-4-hydroxy-3-isoquinolinyl)carbonyl]-glycine i.e. FG-2216, BIQ, or IOX3, an isoquinoline derivative, that inhibits BBOX and other 2-oxoglutarate dependent oxygenases through chelating active site Fe(II) and interacting with specific active site residues. Time course of Zn(II) ejection from BBOX by the inhibitors Homo sapiens
1.14.11.1 thiram
-
 Homo sapiens

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.14.11.1 Fe2+ dependent on, required for catalysis, bound to the active site Homo sapiens
1.14.11.1 Zn2+ dependent on, ejection of structural zinc, e.g. by Selenium and sulfur based compounds, leads to inhibition of the enzyme, an undisrupted N-terminal zinc binding domain of BBOX is required for catalysis Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.11.1 4-trimethylammoniobutanoate + 2-oxoglutarate + O2 Homo sapiens
-
 3-hydroxy-4-trimethylammoniobutanoate + succinate + CO2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.11.1 Homo sapiens O75936
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.11.1 4-trimethylammoniobutanoate + 2-oxoglutarate + O2
-
 Homo sapiens 3-hydroxy-4-trimethylammoniobutanoate + succinate + CO2
-
 ?

Subunits

EC Number Subunits Comment Organism
1.14.11.1 homodimer BBOX is a homodimer comprising of catalytic domain where Fe(II) and substrates bind and zinc binding domain Homo sapiens

Synonyms

EC Number Synonyms Comment Organism
1.14.11.1 BBOX
-
 Homo sapiens
1.14.11.1 gamma-butyrobetaine hydroxylase
-
 Homo sapiens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.14.11.1 22
-
 assay at room temperature Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.14.11.1 7
-
 assay at Homo sapiens

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
1.14.11.1 0.00083
-
 pH 7.0, 22°C, 20 min Homo sapiens ebselen
1.14.11.1 0.0029
-
 pH 7.0, 22°C, 20 min Homo sapiens benzeneselenenyl chloride
1.14.11.1 0.003
-
 pH 7.0, 22°C, 20 min Homo sapiens benzeneselenenyl bromide
1.14.11.1 0.0047
-
 pH 7.0, 22°C, 20 min Homo sapiens benzeneseleninic acid
1.14.11.1 0.0079
-
 pH 7.0, 22°C, 20 min Homo sapiens thiram
1.14.11.1 0.019
-
 pH 7.0, 22°C, 20 min Homo sapiens benzeneselenenic acid
1.14.11.1 0.032
-
 pH 7.0, 22°C, 20 min Homo sapiens N-[(1-chloro-4-hydroxy-3-isoquinolinyl)carbonyl]-glycine
1.14.11.1 0.042
-
 pH 7.0, 22°C, 20 min Homo sapiens diphenyldiselane

General Information

EC Number General Information Comment Organism
1.14.11.1 evolution the enzyme belongs to the 2-oxoglutarate/Fe(II) dependent oxygenase family Homo sapiens
1.14.11.1 metabolism the enzyme catalyses the second, essential step of carnitine biosynthesis Homo sapiens