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Literature summary extracted from

  • Zafar, H.; Hayat, M.; Saied, S.; Khan, M.; Salar, U.; Malik, R.; Choudhary, M.I.; Khan, K.M.
    Xanthine oxidase inhibitory activity of nicotino/isonicotinohydrazides a systematic approach from in vitro, in silico to in vivo studies (2017), Bioorg. Med. Chem., 25, 2351-2371 .
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.17.3.2 additional information xanthine oxidase inhibitory activity of nicotino/isonicotinohydrazides, in vitro, in silico, and in vivo studies, overview. No inhibition by N'-[(E)-(2-nitrophenyl)methylidene]pyridine-3-carbohydrazide, N'-[(E)-(4-nitrophenyl)methylidene]pyridine-3-carbohydrazide, N'-[(E)-(3-hydroxyphenyl)methylidene]pyridine-3-carbohydrazide, N'-[(E)-(2,3,4-trihydroxyphenyl)methylidene]pyridine-3-carbohydrazide, N'-[(E)-(2-ethoxyphenyl)methylidene]pyridine-3-carbohydrazide, N'-[(E)-(2-methoxyphenyl)methylidene]pyridine-3-carbohydrazide, N'-[(E)-(3,4-dimethoxyphenyl)methylidene]pyridine-3-carbohydrazide, N'-[(E)-(2,3,4-trimethoxyphenyl)methylidene]pyridine-3-carbohydrazide, N'-[(E)-(2-hydroxy-6-methoxyphenyl)methylidene]pyridine-3-carbohydrazide, N'-[(E)-(2-hydroxy-5-methoxyphenyl)methylidene]pyridine-3-carbohydrazide, N'-[(E)-(2,4-dichlorophenyl)methylidene]pyridine-3-carbohydrazide, N'-[(E)-(2-hydroxy-5-methylphenyl)methylidene]pyridine-3-carbohydrazide, N'-[(E)-[4-(methylsulfanyl)phenyl]methylidene]pyridine-3-carbohydrazide, N'-[(E)-[4-(dimethylamino)phenyl]methylidene]pyridine-3-carbohydrazide, N'-[(E)-phenylmethylidene]pyridine-4-carbohydrazide, N'-[(E)-(2-nitrophenyl)methylidene]pyridine-4-carbohydrazide, N'-[(E)-(4-nitrophenyl)methylidene]pyridine-4-carbohydrazide, N'-[(E)-(4-hydroxyphenyl)methylidene]pyridine-4-carbohydrazide, N'-[(E)-(3-hydroxyphenyl)methylidene]pyridine-4-carbohydrazide, N'-[(E)-(2,4-dihydroxyphenyl)methylidene]pyridine-4-carbohydrazide, N'-[(E)-(2-ethoxyphenyl)methylidene]pyridine-4-carbohydrazide, N'-[(E)-(3,4-diethoxyphenyl)methylidene]pyridine-4-carbohydrazide, N'-[(E)-(3,4-dimethoxyphenyl)methylidene]pyridine-4-carbohydrazide, N'-[(E)-(4-ethoxy-3-hydroxyphenyl)methylidene]pyridine-4-carbohydrazide, N'-[(E)-(3-hydroxy-4-methoxyphenyl)methylidene]pyridine-4-carbohydrazide, N'-[(E)-(2-hydroxy-5-methoxyphenyl)methylidene]pyridine-4-carbohydrazide, N'-[(E)-(2-chlorophenyl)methylidene]pyridine-4-carbohydrazide, N'-[(E)-(2-fluorophenyl)methylidene]pyridine-4-carbohydrazide, N'-[(E)-(2,4-dichlorophenyl)methylidene]pyridine-4-carbohydrazide, N'-[(E)-(2-chloro-5-nitrophenyl)methylidene]pyridine-4-carbohydrazide, N'-[(E)-(2-hydroxyphenyl)methylidene]pyridine-4-carbohydrazide, N'-[(E)-[4-(methylsulfanyl)phenyl]methylidene]pyridine-4-carbohydrazide, and N'-[(E)-[4-(dimethylamino)phenyl]methylidene]pyridine-4-carbohydrazide Bos taurus
1.17.3.2 N'-[(E)-(2,4,6-trihydroxyphenyl)methylidene]pyridine-4-carbohydrazide
-
 Bos taurus
1.17.3.2 N'-[(E)-(2-bromophenyl)methylidene]pyridine-3-carbohydrazide
-
 Bos taurus
1.17.3.2 N'-[(E)-(2-fluorophenyl)methylidene]pyridine-3-carbohydrazide
-
 Bos taurus
1.17.3.2 N'-[(E)-(2-hydroxy-3-methoxyphenyl)methylidene]pyridine-3-carbohydrazide
-
 Bos taurus
1.17.3.2 N'-[(E)-(2-hydroxy-3-methoxyphenyl)methylidene]pyridine-4-carbohydrazide
-
 Bos taurus
1.17.3.2 N'-[(E)-(2-methylphenyl)methylidene]pyridine-3-carbohydrazide
-
 Bos taurus
1.17.3.2 N'-[(E)-(3,4-dihydroxyphenyl)methylidene]pyridine-3-carbohydrazide
-
 Bos taurus
1.17.3.2 N'-[(E)-(3,4-dihydroxyphenyl)methylidene]pyridine-4-carbohydrazide
-
 Bos taurus
1.17.3.2 N'-[(E)-(3,5-dihydroxyphenyl)methylidene]pyridine-3-carbohydrazide
-
 Bos taurus
1.17.3.2 N'-[(E)-(3-chlorophenyl)methylidene]pyridine-3-carbohydrazide
-
 Bos taurus
1.17.3.2 N'-[(E)-(3-chlorophenyl)methylidene]pyridine-4-carbohydrazide
-
 Bos taurus
1.17.3.2 N'-[(E)-(3-ethoxy-2-hydroxyphenyl)methylidene]pyridine-3-carbohydrazide
-
 Bos taurus
1.17.3.2 N'-[(E)-(3-ethoxy-2-hydroxyphenyl)methylidene]pyridine-4-carbohydrazide
-
 Bos taurus
1.17.3.2 N'-[(E)-(3-nitrophenyl)methylidene]pyridine-3-carbohydrazide
-
 Bos taurus
1.17.3.2 N'-[(E)-(3-nitrophenyl)methylidene]pyridine-4-carbohydrazide
-
 Bos taurus
1.17.3.2 N'-[(E)-(4-chlorophenyl)methylidene]pyridine-3-carbohydrazide
-
 Bos taurus
1.17.3.2 N'-[(E)-(4-chlorophenyl)methylidene]pyridine-4-carbohydrazide
-
 Bos taurus
1.17.3.2 N'-[(E)-(4-ethoxyphenyl)methylidene]pyridine-3-carbohydrazide
-
 Bos taurus
1.17.3.2 N'-[(E)-(4-ethoxyphenyl)methylidene]pyridine-4-carbohydrazide
-
 Bos taurus
1.17.3.2 N'-[(E)-(4-hydroxyphenyl)methylidene]pyridine-3-carbohydrazide
-
 Bos taurus
1.17.3.2 N'-[(E)-phenylmethylidene]pyridine-3-carbohydrazide
-
 Bos taurus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.17.3.2 hypoxanthine + NAD+ + H2O Bos taurus
-
 xanthine + NADH + H+
-
 ?
1.17.3.2 xanthine + H2O + O2 Bos taurus
-
 urate + H2O2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.17.3.2 Bos taurus P80457
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.17.3.2 commercial preparation
-
 Bos taurus
-
1.17.3.2 milk
-
 Bos taurus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.17.3.2 hypoxanthine + NAD+ + H2O
-
 Bos taurus xanthine + NADH + H+
-
 ?
1.17.3.2 xanthine + H2O + O2
-
 Bos taurus urate + H2O2
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.17.3.2 30
-
 assay at Bos taurus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.17.3.2 7.4
-
 assay at Bos taurus

Cofactor

EC Number Cofactor Comment Organism Structure
1.17.3.2 NAD+
-
 Bos taurus

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
1.17.3.2 0.0009
-
 pH 7.4, 30°C Bos taurus N'-[(E)-(2,4,6-trihydroxyphenyl)methylidene]pyridine-4-carbohydrazide
1.17.3.2 0.01
-
 pH 7.4, 30°C Bos taurus N'-[(E)-(3,4-dihydroxyphenyl)methylidene]pyridine-3-carbohydrazide
1.17.3.2 0.0124
-
 pH 7.4, 30°C Bos taurus N'-[(E)-(3,4-dihydroxyphenyl)methylidene]pyridine-4-carbohydrazide
1.17.3.2 0.021
-
 pH 7.4, 30°C Bos taurus N'-[(E)-(2-hydroxy-3-methoxyphenyl)methylidene]pyridine-4-carbohydrazide
1.17.3.2 0.0211
-
 pH 7.4, 30°C Bos taurus N'-[(E)-phenylmethylidene]pyridine-3-carbohydrazide
1.17.3.2 0.055
-
 pH 7.4, 30°C Bos taurus N'-[(E)-(3-ethoxy-2-hydroxyphenyl)methylidene]pyridine-4-carbohydrazide
1.17.3.2 0.161
-
 pH 7.4, 30°C Bos taurus N'-[(E)-(3,5-dihydroxyphenyl)methylidene]pyridine-3-carbohydrazide
1.17.3.2 0.174
-
 pH 7.4, 30°C Bos taurus N'-[(E)-(2-fluorophenyl)methylidene]pyridine-3-carbohydrazide
1.17.3.2 0.174
-
 pH 7.4, 30°C Bos taurus N'-[(E)-(3-nitrophenyl)methylidene]pyridine-4-carbohydrazide
1.17.3.2 0.1755
-
 pH 7.4, 30°C Bos taurus N'-[(E)-(2-hydroxy-3-methoxyphenyl)methylidene]pyridine-3-carbohydrazide
1.17.3.2 0.179
-
 pH 7.4, 30°C Bos taurus N'-[(E)-(4-chlorophenyl)methylidene]pyridine-4-carbohydrazide
1.17.3.2 0.18
-
 pH 7.4, 30°C Bos taurus N'-[(E)-(2-methylphenyl)methylidene]pyridine-3-carbohydrazide
1.17.3.2 0.184
-
 pH 7.4, 30°C Bos taurus N'-[(E)-(3-nitrophenyl)methylidene]pyridine-3-carbohydrazide
1.17.3.2 0.184
-
 pH 7.4, 30°C Bos taurus N'-[(E)-(4-hydroxyphenyl)methylidene]pyridine-3-carbohydrazide
1.17.3.2 0.195
-
 pH 7.4, 30°C Bos taurus N'-[(E)-(4-chlorophenyl)methylidene]pyridine-3-carbohydrazide
1.17.3.2 0.198
-
 pH 7.4, 30°C Bos taurus N'-[(E)-(2-bromophenyl)methylidene]pyridine-3-carbohydrazide
1.17.3.2 0.205
-
 pH 7.4, 30°C Bos taurus N'-[(E)-(3-chlorophenyl)methylidene]pyridine-3-carbohydrazide
1.17.3.2 0.206
-
 pH 7.4, 30°C Bos taurus N'-[(E)-(3-chlorophenyl)methylidene]pyridine-4-carbohydrazide
1.17.3.2 0.242
-
 pH 7.4, 30°C Bos taurus N'-[(E)-(4-ethoxyphenyl)methylidene]pyridine-3-carbohydrazide
1.17.3.2 0.264
-
 pH 7.4, 30°C Bos taurus N'-[(E)-(3-ethoxy-2-hydroxyphenyl)methylidene]pyridine-3-carbohydrazide
1.17.3.2 0.33
-
 pH 7.4, 30°C Bos taurus N'-[(E)-(4-ethoxyphenyl)methylidene]pyridine-4-carbohydrazide