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Literature summary extracted from

  • Ben Khaled, H.; Ktari, N.; Siala, R.; Hmidet, N.; Bayoudh, A.; Nasri, M.; Ghorbel, S.
    A heat-stable Cu/Zn superoxide dismutase from the viscera of sardinelle (Sardinella aurita) purification and biochemical characterization (2014), Biologia (Poland), 69, 1770-1776 .
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.15.1.1 H2O2 90% inhibition at 5 mM Sardinella aurita
1.15.1.1 KCN complete inhibition at 5 mM Sardinella aurita

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.15.1.1 Cu2+ a Cu/Zn SOD Sardinella aurita
1.15.1.1 Zn2+ a Cu/Zn SOD Sardinella aurita

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.15.1.1 40000
-
 native enzyme, gel filtration Sardinella aurita

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.15.1.1 2 superoxide + 2 H+ Sardinella aurita
-
 O2 + H2O2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.15.1.1 Sardinella aurita
-
 from the fish market of Sfax City, Tunisia
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.15.1.1 native enzyme from sardinelle viscera to homogeneity 7.17fold by heat treatment at 65°C for 15 min, ammonium sulfate fractionation, and gel filtration Sardinella aurita

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.15.1.1 228.19
-
 purified native enzyme, pH 7.0, 37°C Sardinella aurita

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.15.1.1 2 superoxide + 2 H+
-
 Sardinella aurita O2 + H2O2
-
 ?
1.15.1.1 additional information SOD activity is measured using a method employing xanthine and xanthine oxidase to generate superoxide radicals which react with 2-(4-iodophenyl)-3-(4-nitrophenol)-5-phenyltetrazolium chloride (INT) to form a red formazan dye. The SOD activity is then measured spectrophotometrically by the degree of inhibition of this reaction by means of the decrease in absorbance at 505 nm, 37°C Sardinella aurita ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.15.1.1 homodimer 2 * 20000, native enzyme, SDS-PAGE Sardinella aurita
1.15.1.1 More the N-terminal sequence of the purified native enzyme is VLKAVCVLKGTGEVT Sardinella aurita

Synonyms

EC Number Synonyms Comment Organism
1.15.1.1 Cu/Zn SOD
-
 Sardinella aurita
1.15.1.1 Cu/Zn superoxide dismutase
-
 Sardinella aurita
1.15.1.1 SOD
-
 Sardinella aurita

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.15.1.1 40
-
-
 Sardinella aurita

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.15.1.1 25 70 activity range, profile overview. Relative activities at 25°C and 50°C are about 80% and 73%, respectively, of maximal activity at 40°C Sardinella aurita

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.15.1.1 50
-
 purified native enzyme, more than 85% of its initial activity is retained after 1 h of incubation. The enzyme is highly stable at temperatures below 50°C Sardinella aurita
1.15.1.1 60
-
 purified native enzyme, 45% of its initial activity is retained after 1 h of incubation Sardinella aurita
1.15.1.1 70
-
 purified native enzyme, 37% of its initial activity is retained after 1 h of incubation Sardinella aurita

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.15.1.1 7
-
-
 Sardinella aurita

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.15.1.1 4 11 activity range, profile overview Sardinella aurita

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.15.1.1 6 9 at pH 6.0 and pH 9.0, the enzyme retains about 78% and 75%, respectively, of maximal activity after 1 h incubation at 37°C Sardinella aurita

General Information

EC Number General Information Comment Organism
1.15.1.1 physiological function superoxide dismutase (SOD) is an enzyme that protects against oxidative stress from superoxide radicals in living cells Sardinella aurita