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Literature summary extracted from

  • Wang, L.; Zhang, J.; Dong, X.; Fu, Z.; Jiang, H.; Zhang, H.
    Identification and functional analysis of anthocyanin biosynthesis genes in Phalaenopsis hybrids (2018), Biol. Plant., 62, 45-54 .
No PubMed abstract available

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.14.81 DNA and amino acid sequence determination and analysis, sequence comparisons, recombinant enzyme overexpression in Petunia hybrida hybrid M1 x R27, which contains a mutated endogenous F3'5'H (HF1), no obvious phenotype Phalaenopsis sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.14.81 dihydrokaempferol + 2 [reduced NADPH-hemoprotein reductase] + 2 O2 Phalaenopsis sp. overall reaction dihydromyricetin + 2 [oxidized NADPH-hemoprotein reductase] + 2 H2O
-
 ?
1.14.14.81 dihydrokaempferol + [reduced NADPH-hemoprotein reductase] + O2 Phalaenopsis sp.
-
 dihydroquercetin + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
1.14.14.81 dihydroquercetin + [reduced NADPH-hemoprotein reductase] + O2 Phalaenopsis sp.
-
 dihydromyricetin + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.14.81 Phalaenopsis hybrid cultivar Q3YAF0 cv. Big Chili
-
1.14.14.81 Phalaenopsis sp.
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.14.14.81 flower bud weak expression at stages 1-3 of bud development Phalaenopsis hybrid cultivar
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.14.81 dihydrokaempferol + 2 [reduced NADPH-hemoprotein reductase] + 2 O2 overall reaction Phalaenopsis sp. dihydromyricetin + 2 [oxidized NADPH-hemoprotein reductase] + 2 H2O
-
 ?
1.14.14.81 dihydrokaempferol + [reduced NADPH-hemoprotein reductase] + O2
-
 Phalaenopsis sp. dihydroquercetin + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
1.14.14.81 dihydroquercetin + [reduced NADPH-hemoprotein reductase] + O2
-
 Phalaenopsis sp. dihydromyricetin + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.14.14.81 F3'5'H
-
 Phalaenopsis sp.

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.14.81 NADPH
-
 Phalaenopsis sp.

General Information

EC Number General Information Comment Organism
1.14.14.81 evolution the F3'5'H gene in Phalaenopsis has a relatively large evolutionary distance to other plant F3'5'Hs Phalaenopsis sp.
1.14.14.81 physiological function overexpression in petunia shows that it has no function in anthocyanin production Phalaenopsis hybrid cultivar
1.14.14.81 physiological function Phalaenopsis genome contains the F3'5'H gene, which is the key gene for violet/blue colour, but the enzyme has no function in anthocyanin production Phalaenopsis sp.