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Literature summary extracted from

  • Takahashi, T.; Kawagishi, S.; Funahashi, H.; Hayashi, N.; Suzuki, T.
    Production and purification of secretory simian cytidine monophosphate-N-acetylneuraminic acid hydroxylase using baculovirus-protein expression system (2015), Biol. Pharm. Bull., 38, 1220-1226 .
    View publication on PubMed

Application

EC Number Application Comment Organism
1.14.18.2 synthesis enzyme CMAH can be used for large-scale production of N-glycolylneuraminic acid Chlorocebus aethiops

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.18.2 functional recombinant expression of secretory His-tagged enzyme, containing an N-terminal secretion signal peptide, in Spodoptera frugiperda Sf9 cells using the baculovirus infection method, large scale enzyme production Chlorocebus aethiops

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.18.2 CMP-N-acetylneuraminate + 2 ferrocytochrome b5 + O2 + 2 H+ Chlorocebus aethiops
-
CMP-N-glycoloylneuraminate + 2 ferricytochrome b5 + H2O
-
?

Organism

EC Number Organism UniProt Comment Textmining
1.14.18.2 Chlorocebus aethiops
-
-
-
1.14.18.2 no activity in Homo sapiens
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
1.14.18.2 glycoprotein the secreted recombinant His-tagged enzyme contains N-glycans Chlorocebus aethiops

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.18.2 recombinant secretory His-tagged enzyme from Spodoptera frugiperda Sf9 cell culture supernatant by affinity chromatography and ultrafiltration Chlorocebus aethiops

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.14.18.2 COS-7 cell
-
Chlorocebus aethiops
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.18.2 CMP-N-acetylneuraminate + 2 ferrocytochrome b5 + O2 + 2 H+
-
Chlorocebus aethiops CMP-N-glycoloylneuraminate + 2 ferricytochrome b5 + H2O
-
?

Subunits

EC Number Subunits Comment Organism
1.14.18.2 ? x + 65000, secreted recombinant His-tagged enzyme, SDS-PAGE Chlorocebus aethiops

Synonyms

EC Number Synonyms Comment Organism
1.14.18.2 CMAH
-
Chlorocebus aethiops
1.14.18.2 CMP Neu5Ac hydroxylase
-
Chlorocebus aethiops
1.14.18.2 cytidine monophosphate-N-acetylneuraminic acid hydroxylase
-
Chlorocebus aethiops

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.14.18.2 37
-
assay at Chlorocebus aethiops

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.14.18.2 7.9
-
assay at Chlorocebus aethiops

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.18.2 cytochrome b5
-
Chlorocebus aethiops

General Information

EC Number General Information Comment Organism
1.14.18.2 additional information sequence-based, three-dimensional enzyme structure modeling, overview Chlorocebus aethiops
1.14.18.2 physiological function the enzyme is essential for N-glycolylneuraminic acid (Neu5Gc) synthesis Chlorocebus aethiops