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Literature summary extracted from

  • Koh, C.Y.; Wetzel, A.B.; de van der Schueren, W.J.; Hol, W.G.
    Comparison of histidine recognition in human and trypanosomatid histidyl-tRNA synthetases (2014), Biochimie, 106, 111-120 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.1.1.21 expressed in Escherichia coli BL21(DE3) cells Homo sapiens

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
6.1.1.21 vapor diffusion method, using 0.1 M Tris pH 8.5, 0.2 M MgCl2 and 25% (w/v) PEG 3350 Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
6.1.1.21 cytosol
-
 Homo sapiens 5829
-
6.1.1.21 cytosol
-
 Trypanosoma brucei 5829
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.1.1.21 ATP + L-histidine + tRNAHis Homo sapiens
-
 AMP + diphosphate + L-histidyl-tRNAHis
-
 ?
6.1.1.21 ATP + L-histidine + tRNAHis Trypanosoma brucei
-
 AMP + diphosphate + L-histidyl-tRNAHis
-
 ?
6.1.1.21 ATP + L-histidine + tRNAHis Trypanosoma brucei CL Brener
-
 AMP + diphosphate + L-histidyl-tRNAHis
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
6.1.1.21 Homo sapiens P12081
-
-
6.1.1.21 Trypanosoma brucei Q4DA54
-
-
6.1.1.21 Trypanosoma brucei CL Brener Q4DA54
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.1.1.21 Ni-NTA column chromatography, and Superdex 200 gel filtration Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.1.1.21 ATP + L-histidine + tRNAHis
-
 Homo sapiens AMP + diphosphate + L-histidyl-tRNAHis
-
 ?
6.1.1.21 ATP + L-histidine + tRNAHis
-
 Trypanosoma brucei AMP + diphosphate + L-histidyl-tRNAHis
-
 ?
6.1.1.21 ATP + L-histidine + tRNAHis
-
 Trypanosoma brucei CL Brener AMP + diphosphate + L-histidyl-tRNAHis
-
 ?

Subunits

EC Number Subunits Comment Organism
6.1.1.21 homodimer
-
 Homo sapiens

Synonyms

EC Number Synonyms Comment Organism
6.1.1.21 HisRS
-
 Homo sapiens
6.1.1.21 HisRS
-
 Trypanosoma brucei
6.1.1.21 Histidyl-tRNA synthetase
-
 Homo sapiens
6.1.1.21 Histidyl-tRNA synthetase
-
 Trypanosoma brucei

Cofactor

EC Number Cofactor Comment Organism Structure
6.1.1.21 ATP
-
 Homo sapiens
6.1.1.21 ATP
-
 Trypanosoma brucei