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Literature summary extracted from

  • Toyama, D.; de Morais, M.; Ramos, F.; Zanphorlin, L.; Tonoli, C.; Balula, A.; de Miranda, F.; Almeida, V.; Marana, S.; Ruller, R.; Murakami, M.; Henrique-Silva, F.
    A novel beta-glucosidase isolated from the microbial metagenome of Lake Poraque (Amazon, Brazil) (2018), Biochim. Biophys. Acta, 1866, 569-579 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.21 expression in Escherichia coli unidentified microorganism

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.2.1.21 to 2.75 A resolution. The predicted aglycone region is surrounded by hydrophobic residues Val220, Pro294, and Ala237. The residues His174 and Trp342 delimit the access to the -1 subsite and are critical to define the active site architecture unidentified microorganism

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.21 additional information
-
 dimer structure, SAXS scattering and energetic analysis unidentified microorganism

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.21 unidentified microorganism A0A2I2LGB3 microbial metagenome of Lake Poraque, Amazon, Brazil
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.21 4-nitrophenyl beta-D-galactopyranoside + H2O
-
 unidentified microorganism 4-nitrophenol + beta-D-galactose
-
 ?
3.2.1.21 4-nitrophenyl beta-D-glucopyranoside + H2O
-
 unidentified microorganism 4-nitrophenol + beta-D-glucose
-
 ?
3.2.1.21 cellobiose + H2O
-
 unidentified microorganism 2 D-glucose
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.21 dimer 2 * 52900, calculated unidentified microorganism

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.21 40
-
-
 unidentified microorganism

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.21 35
-
 96% of maximum activity unidentified microorganism

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.21 40
-
 30 min, 60% residual activity unidentified microorganism
3.2.1.21 46.7
-
 melting temperature unidentified microorganism

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.21 6
-
-
 unidentified microorganism

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.21 5.5 6.5
-
 unidentified microorganism