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Literature summary extracted from

  • Contesini, F.J.; Liberato, M.V.; Rubio, M.V.; Calzado, F.; Zubieta, M.P.; Riano-Pachon, D.M.; Squina, F.M.; Bracht, F.; Skaf, M.S.; Damasio, A.R.
    Structural and functional characterization of a highly secreted alpha-L-arabinofuranosidase (GH62) from Aspergillus nidulans grown on sugarcane bagasse (2017), Biochim. Biophys. Acta, 1865, 1758-1769 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.55 expressed in Escherichia coli Arctic cells Aspergillus nidulans

Protein Variants

EC Number Protein Variants Comment Organism
3.2.1.55 N83Q the mutant is not glycosylated but shows about wild type activity Aspergillus nidulans
3.2.1.55 Y312F the mutant shows significantly reduced activity compared to the wild type enzyme Aspergillus nidulans
3.2.1.55 Y312S the mutant shows significantly reduced activity compared to the wild type enzyme Aspergillus nidulans

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.55 Aspergillus nidulans Q5AUX2
-
-
3.2.1.55 Aspergillus nidulans A773 Q5AUX2
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.2.1.55 glycoprotein the enzyme is N-glycosylated at asparagine 83 Aspergillus nidulans

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.55
-
 Aspergillus nidulans

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.55 culture condition:sugarcane bagasse-grown cell
-
 Aspergillus nidulans
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.55 4-nitrophenyl alpha-L-arabinofuranoside + H2O
-
 Aspergillus nidulans 4-nitrophenol + alpha-L-arabinofuranose
-
 ?
3.2.1.55 4-nitrophenyl alpha-L-arabinofuranoside + H2O
-
 Aspergillus nidulans A773 4-nitrophenol + alpha-L-arabinofuranose
-
 ?
3.2.1.55 additional information no activity with debranched arabinan, sugar beet arabinan, linear arabinan, larch arabinogalactan Aspergillus nidulans ?
-
 ?
3.2.1.55 additional information no activity with debranched arabinan, sugar beet arabinan, linear arabinan, larch arabinogalactan Aspergillus nidulans A773 ?
-
 ?
3.2.1.55 rye arabinoxylan + H2O
-
 Aspergillus nidulans arabinose + ?
-
 ?
3.2.1.55 rye arabinoxylan + H2O
-
 Aspergillus nidulans A773 arabinose + ?
-
 ?
3.2.1.55 wheat arabinoxylan + H2O
-
 Aspergillus nidulans arabinose + ?
-
 ?
3.2.1.55 wheat arabinoxylan + H2O
-
 Aspergillus nidulans A773 arabinose + ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.55 ? x * 35500, SDS-PAGE Aspergillus nidulans

Synonyms

EC Number Synonyms Comment Organism
3.2.1.55 alpha-L-arabinofuranosidase
-
 Aspergillus nidulans
3.2.1.55 AN7908
-
 Aspergillus nidulans
3.2.1.55 GH62
-
 Aspergillus nidulans

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.55 60 65
-
 Aspergillus nidulans

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.55 354.17
-
 rye arabinoxylan at pH 5.5 and 60°C Aspergillus nidulans
3.2.1.55 361.74
-
 wheat arabinoxylan at pH 5.5 and 60°C Aspergillus nidulans

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.55 5.5
-
-
 Aspergillus nidulans

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.55 4.5 6
-
 Aspergillus nidulans