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Literature summary extracted from

  • Harris, S.; Devendran, S.; Alves, J.; Mythen, S.; Hylemon, P.; Ridlon, J.
    Identification of a gene encoding a flavoprotein involved in bile acid metabolism by the human gut bacterium Clostridium scindens ATCC 35704 (2018), Biochim. Biophys. Acta, 1863, 276-283 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.3.1.114 expressionj in Escherichia coli [Clostridium] scindens
1.3.1.114 overexpression in Escherichia coli [Clostridium] scindens

General Stability

EC Number General Stability Organism
1.3.1.114 purified protein is stable only for less than 12 h, then precipitates [Clostridium] scindens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.3.1.114 12alpha-hydroxy-3-oxochol-4-en-24-oyl-CoA + NAD+ [Clostridium] scindens
-
12alpha-hydroxy-3-oxochol-4,6-dien-24-oyl-CoA + NADH + H+
-
?
1.3.1.114 12alpha-hydroxy-3-oxochol-4-en-24-oyl-CoA + NAD+ [Clostridium] scindens the enzyme participates in the bile acid 7alpha-dehydroxylation pathway. It catalyses two subsequent reductions of the double bonds within the bile acid A/B rings, following 7alpha-dehydration 12alpha-hydroxy-3-oxochol-4,6-dien-24-oyl-CoA + NADH + H+
-
?
1.3.1.114 12alpha-hydroxy-3-oxochol-4-en-24-oyl-CoA + NAD+ [Clostridium] scindens ATCC 35704
-
12alpha-hydroxy-3-oxochol-4,6-dien-24-oyl-CoA + NADH + H+
-
?
1.3.1.114 12alpha-hydroxy-3-oxochol-4-en-24-oyl-CoA + NAD+ [Clostridium] scindens ATCC 35704 the enzyme participates in the bile acid 7alpha-dehydroxylation pathway. It catalyses two subsequent reductions of the double bonds within the bile acid A/B rings, following 7alpha-dehydration 12alpha-hydroxy-3-oxochol-4,6-dien-24-oyl-CoA + NADH + H+
-
?
1.3.1.114 12alpha-hydroxy-3-oxocholan-24-oyl-CoA + NAD+ [Clostridium] scindens
-
12alpha-hydroxy-3-oxochol-4-en-24-oyl-CoA + NADH + H+
-
?
1.3.1.114 12alpha-hydroxy-3-oxocholan-24-oyl-CoA + NAD+ [Clostridium] scindens the enzyme participates in the bile acid 7alpha-dehydroxylation pathway. It catalyses two subsequent reductions of the double bonds within the bile acid A/B rings, following 7alpha-dehydration 12alpha-hydroxy-3-oxochol-4-en-24-oyl-CoA + NADH + H+
-
?
1.3.1.114 12alpha-hydroxy-3-oxocholan-24-oyl-CoA + NAD+ [Clostridium] scindens ATCC 35704
-
12alpha-hydroxy-3-oxochol-4-en-24-oyl-CoA + NADH + H+
-
?
1.3.1.114 12alpha-hydroxy-3-oxocholan-24-oyl-CoA + NAD+ [Clostridium] scindens ATCC 35704 the enzyme participates in the bile acid 7alpha-dehydroxylation pathway. It catalyses two subsequent reductions of the double bonds within the bile acid A/B rings, following 7alpha-dehydration 12alpha-hydroxy-3-oxochol-4-en-24-oyl-CoA + NADH + H+
-
?
1.3.1.114 3-oxochol-4-en-24-oyl-CoA + NAD+ [Clostridium] scindens
-
3-oxochol-4,6-dien-24-oyl-CoA + NADH + H+
-
?
1.3.1.114 3-oxochol-4-en-24-oyl-CoA + NAD+ [Clostridium] scindens the enzyme participates in the bile acid 7alpha-dehydroxylation pathway. It catalyses two subsequent reductions of the double bonds within the bile acid A/B rings, following 7alpha-dehydration 3-oxochol-4,6-dien-24-oyl-CoA + NADH + H+
-
?
1.3.1.114 3-oxochol-4-en-24-oyl-CoA + NAD+ [Clostridium] scindens ATCC 35704
-
3-oxochol-4,6-dien-24-oyl-CoA + NADH + H+
-
?
1.3.1.114 3-oxocholan-24-oyl-CoA + NAD+ [Clostridium] scindens
-
3-oxochol-4-en-24-oyl-CoA + NADH + H+
-
?
1.3.1.114 3-oxocholan-24-oyl-CoA + NAD+ [Clostridium] scindens the enzyme participates in the bile acid 7alpha-dehydroxylation pathway. It catalyses two subsequent reductions of the double bonds within the bile acid A/B rings, following 7alpha-dehydration 3-oxochol-4-en-24-oyl-CoA + NADH + H+
-
?

Organism

EC Number Organism UniProt Comment Textmining
1.3.1.114 [Clostridium] scindens B0NAQ4
-
-
1.3.1.114 [Clostridium] scindens ATCC 35704 B0NAQ4
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.3.1.114
-
[Clostridium] scindens

Storage Stability

EC Number Storage Stability Organism
1.3.1.114 -20°C, 50% glycerol does not improve stability [Clostridium] scindens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.1.114 12alpha-hydroxy-3-oxochol-4-en-24-oyl-CoA + NAD+
-
[Clostridium] scindens 12alpha-hydroxy-3-oxochol-4,6-dien-24-oyl-CoA + NADH + H+
-
?
1.3.1.114 12alpha-hydroxy-3-oxochol-4-en-24-oyl-CoA + NAD+ the enzyme participates in the bile acid 7alpha-dehydroxylation pathway. It catalyses two subsequent reductions of the double bonds within the bile acid A/B rings, following 7alpha-dehydration [Clostridium] scindens 12alpha-hydroxy-3-oxochol-4,6-dien-24-oyl-CoA + NADH + H+
-
?
1.3.1.114 12alpha-hydroxy-3-oxochol-4-en-24-oyl-CoA + NAD+
-
[Clostridium] scindens ATCC 35704 12alpha-hydroxy-3-oxochol-4,6-dien-24-oyl-CoA + NADH + H+
-
?
1.3.1.114 12alpha-hydroxy-3-oxochol-4-en-24-oyl-CoA + NAD+ the enzyme participates in the bile acid 7alpha-dehydroxylation pathway. It catalyses two subsequent reductions of the double bonds within the bile acid A/B rings, following 7alpha-dehydration [Clostridium] scindens ATCC 35704 12alpha-hydroxy-3-oxochol-4,6-dien-24-oyl-CoA + NADH + H+
-
?
1.3.1.114 12alpha-hydroxy-3-oxocholan-24-oyl-CoA + NAD+
-
[Clostridium] scindens 12alpha-hydroxy-3-oxochol-4-en-24-oyl-CoA + NADH + H+
-
?
1.3.1.114 12alpha-hydroxy-3-oxocholan-24-oyl-CoA + NAD+ the enzyme participates in the bile acid 7alpha-dehydroxylation pathway. It catalyses two subsequent reductions of the double bonds within the bile acid A/B rings, following 7alpha-dehydration [Clostridium] scindens 12alpha-hydroxy-3-oxochol-4-en-24-oyl-CoA + NADH + H+
-
?
1.3.1.114 12alpha-hydroxy-3-oxocholan-24-oyl-CoA + NAD+
-
[Clostridium] scindens ATCC 35704 12alpha-hydroxy-3-oxochol-4-en-24-oyl-CoA + NADH + H+
-
?
1.3.1.114 12alpha-hydroxy-3-oxocholan-24-oyl-CoA + NAD+ the enzyme participates in the bile acid 7alpha-dehydroxylation pathway. It catalyses two subsequent reductions of the double bonds within the bile acid A/B rings, following 7alpha-dehydration [Clostridium] scindens ATCC 35704 12alpha-hydroxy-3-oxochol-4-en-24-oyl-CoA + NADH + H+
-
?
1.3.1.114 3-oxochol-4-en-24-oyl-CoA + NAD+
-
[Clostridium] scindens 3-oxochol-4,6-dien-24-oyl-CoA + NADH + H+
-
?
1.3.1.114 3-oxochol-4-en-24-oyl-CoA + NAD+ the enzyme participates in the bile acid 7alpha-dehydroxylation pathway. It catalyses two subsequent reductions of the double bonds within the bile acid A/B rings, following 7alpha-dehydration [Clostridium] scindens 3-oxochol-4,6-dien-24-oyl-CoA + NADH + H+
-
?
1.3.1.114 3-oxochol-4-en-24-oyl-CoA + NAD+
-
[Clostridium] scindens ATCC 35704 3-oxochol-4,6-dien-24-oyl-CoA + NADH + H+
-
?
1.3.1.114 3-oxocholan-24-oyl-CoA + NAD+
-
[Clostridium] scindens 3-oxochol-4-en-24-oyl-CoA + NADH + H+
-
?
1.3.1.114 3-oxocholan-24-oyl-CoA + NAD+ the enzyme participates in the bile acid 7alpha-dehydroxylation pathway. It catalyses two subsequent reductions of the double bonds within the bile acid A/B rings, following 7alpha-dehydration [Clostridium] scindens 3-oxochol-4-en-24-oyl-CoA + NADH + H+
-
?

Subunits

EC Number Subunits Comment Organism
1.3.1.114 ? x * 44500, calculated from sequence, x * 46500, SDS-PAGE, recombinant protein with streptavidin-tag [Clostridium] scindens

Synonyms

EC Number Synonyms Comment Organism
1.3.1.114 BaiN
-
[Clostridium] scindens
1.3.1.114 CLOSCI_00523
-
[Clostridium] scindens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.3.1.114 37
-
assay at [Clostridium] scindens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.3.1.114 7
-
assay at [Clostridium] scindens

Cofactor

EC Number Cofactor Comment Organism Structure
1.3.1.114 FAD
-
[Clostridium] scindens
1.3.1.114 flavin flavoenzyme [Clostridium] scindens

General Information

EC Number General Information Comment Organism
1.3.1.114 metabolism the enzyme participates in the bile acid 7alpha-dehydroxylation pathway. It catalyses two subsequent reductions of the double bonds within the bile acid A/B rings, following 7alpha-dehydration [Clostridium] scindens
1.3.1.114 physiological function enzyme participates in the bile acid 7alpha-dehydroxylation pathway, catalyzing two subsequent reductions of the double bonds within the bile acid A/B rings, following 7alpha-dehydration [Clostridium] scindens