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Literature summary extracted from

  • Machida, S.; Shiraiwa, Y.; Suzuki, I.
    Construction of a cyanobacterium synthesizing cyclopropane fatty acids (2016), Biochim. Biophys. Acta, 1861, 980-987 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.19.27 gene decC2, recombinant expression in Synechocystis sp. PCC 6803 in wild-type and desA-/des- mutant strains, coexpression with gene cfa, fatty acid compositions of total lipids from Synechocystis sp. wild-type and transformants, synthesis of C17:1cycloDELTA9 by coexpression of the cfa and desC2 genes in the wild-type and desA-/desD- strains of Synechocystis, cell growth, overview Nostoc sp. 36

Protein Variants

EC Number Protein Variants Comment Organism
1.14.19.27 additional information synthesis of cyclopropane fatty acids in the cyanobacterium Synechocystis sp. PCC 6803 by expressing the cyclopropane fatty acid synthase from Escherichia coli to produce fatty acids that are fluid and stable in response to oxidization. To increase the yield of cyclopropane fatty acids in vivo, the desC2 gene from Nostoc sp. is also expressed, expression of the desC2 gene for sn-2 specific DELTA9 desaturase positively affects growth at low temperatures, producing C16:1DELTA9 synthesis in the desA?/desD? strain, via promotion of various cellular processes, with the exceptions of photosynthesis and respiration Nostoc sp. 36

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.19.27 a 1-acyl-2-palmitoyl-[glycerolipid] + 2 reduced ferredoxin [iron-sulfur] cluster + O2 + 2 H+ Nostoc sp. 36
-
 a 1-acyl-2-palmitoleoyl-[glycerolipid] + 2 oxidized ferredoxin [iron-sulfur] cluster + 2 H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.19.27 Nostoc sp. 36 Q704E9
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.19.27 a 1-acyl-2-palmitoyl-[glycerolipid] + 2 reduced ferredoxin [iron-sulfur] cluster + O2 + 2 H+
-
 Nostoc sp. 36 a 1-acyl-2-palmitoleoyl-[glycerolipid] + 2 oxidized ferredoxin [iron-sulfur] cluster + 2 H2O
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.14.19.27 Cfa
-
 Nostoc sp. 36
1.14.19.27 DesC2
-
 Nostoc sp. 36
1.14.19.27 sn-2 specific DELTA9 desaturase
-
 Nostoc sp. 36

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.19.27 Ferredoxin
-
 Nostoc sp. 36