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Literature summary extracted from

  • Bergner, T.; Pavkov-Keller, T.; Kreuzer, K.; Kowaliuk, J.; Plank, M.; Runggatscher, K.; Turrini, N.G.; Zucol, B.; Wallner, S.; Faber, K.; Gruber, K.; Macheroux, P.
    Anthranoyl-CoA monooxygenase/reductase from Azoarcus evansii possesses both FMN and FAD in two distinct and independent active sites (2015), Biochim. Biophys. Acta, 1854, 890-896 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.13.40 expression in Escherichia coli BL21 Aromatoleum evansii

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.14.13.40 165000
-
-
 Aromatoleum evansii

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.13.40 anthraniloyl-CoA + 2 NAD(P)H + 2 H+ + O2 Aromatoleum evansii the enzyme participates in a pathway for the degradation of aromatic compounds in Azoarcus evansii 2-amino-5-oxocyclohex-1-enecarboxyl-CoA + H2O + 2 NAD(P)+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.13.40 Aromatoleum evansii
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.13.40 anthraniloyl-CoA + 2 NAD(P)H + 2 H+ + O2 the enzyme participates in a pathway for the degradation of aromatic compounds in Azoarcus evansii Aromatoleum evansii 2-amino-5-oxocyclohex-1-enecarboxyl-CoA + H2O + 2 NAD(P)+
-
 ?
1.14.13.40 anthraniloyl-CoA + 2 NAD(P)H + 2 H+ + O2 the enzyme catalyzes the monooxygenation of anthranoyl-CoA to 5-hydroxyl-2-aminobenzoyl-CoA and the subsequent reduction to the dearomatized product 2-amino-5-oxo-cyclohex-1-ene-1-carbonyl-CoA. The two reactions occur in separate domains, termed the monooxygenase and reductase domain. Binding studies with 2-aminobenzoyl-CoA and p-hydroxybenzaldehyde as probes for the monooxygenase and reductase domain, respectively, indicate that two functionally distinct and independent active sites exist Aromatoleum evansii 2-amino-5-oxocyclohex-1-enecarboxyl-CoA + H2O + 2 NAD(P)+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.14.13.40 homodimer it is proposed that the monooxygenase and reductase domain of opposite peptide chains are involved in the transformation of anthranoyl-CoA to 2-amino-5-oxo-cyclohex-1-ene-1-carbonyl-CoA Aromatoleum evansii

Synonyms

EC Number Synonyms Comment Organism
1.14.13.40 ACMR
-
 Aromatoleum evansii
1.14.13.40 anthranoyl-CoA monooxygenase/reductase
-
 Aromatoleum evansii

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.13.40 FAD the monooxygenase domain contains FAD Aromatoleum evansii
1.14.13.40 FMN the reductase domain utilizes FMN for the reduction of the intermediate 5-hydroxyl-2-aminobenzoyl-CoA Aromatoleum evansii

General Information

EC Number General Information Comment Organism
1.14.13.40 metabolism the enzyme participates in a pathway for the degradation of aromatic compounds in Azoarcus evansii Aromatoleum evansii