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Literature summary extracted from

  • Kang, D.; Ridlon, J.; Moore II, D.; Barnes, S.; Hylemon, P.
    Clostridium scindens baiCD and baiH genes encode stereo-specific 7alpha/7beta-hydroxy-3-oxo-DELTA4-cholenoic acid oxidoreductases (2008), Biochim. Biophys. Acta, 1781, 16-25 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.3.1.115 expression in Escherichia coli [Clostridium] scindens
1.3.1.116 expression in Escherichia coli [Clostridium] scindens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.3.1.116 72000
-
 SDS-PAGE [Clostridium] scindens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.3.1.115 7alpha,12alpha-dihydroxy-3-oxochol-24-oyl-CoA + NAD+ [Clostridium] scindens
-
 7alpha,12alpha-dihydroxy-3-oxochol-4-en-24-oyl-CoA + NADH + H+
-
 ?
1.3.1.115 7alpha,12alpha-dihydroxy-3-oxochol-24-oyl-CoA + NAD+ [Clostridium] scindens the enzyme participates in the bile acid 7alpha-dehydroxylation pathway 7alpha,12alpha-dihydroxy-3-oxochol-4-en-24-oyl-CoA + NADH + H+
-
 ?
1.3.1.115 7alpha,12alpha-dihydroxy-3-oxochol-24-oyl-CoA + NAD+ [Clostridium] scindens JCM 10418
-
 7alpha,12alpha-dihydroxy-3-oxochol-4-en-24-oyl-CoA + NADH + H+
-
 ?
1.3.1.115 7alpha,12alpha-dihydroxy-3-oxochol-24-oyl-CoA + NAD+ [Clostridium] scindens VPI 12708 the enzyme participates in the bile acid 7alpha-dehydroxylation pathway 7alpha,12alpha-dihydroxy-3-oxochol-4-en-24-oyl-CoA + NADH + H+
-
 ?
1.3.1.115 7alpha-hydroxy-3-oxochol-24-oyl-CoA + NAD+ [Clostridium] scindens
-
 7alpha-hydroxy-3-oxochol-4-en-24-oyl-CoA + NADH + H+
-
 ?
1.3.1.115 7alpha-hydroxy-3-oxochol-24-oyl-CoA + NAD+ [Clostridium] scindens the enzyme participates in the bile acid 7alpha-dehydroxylation pathway 7alpha-hydroxy-3-oxochol-4-en-24-oyl-CoA + NADH + H+
-
 ?
1.3.1.115 7alpha-hydroxy-3-oxochol-24-oyl-CoA + NAD+ [Clostridium] scindens JCM 10418
-
 7alpha-hydroxy-3-oxochol-4-en-24-oyl-CoA + NADH + H+
-
 ?
1.3.1.115 7alpha-hydroxy-3-oxochol-24-oyl-CoA + NAD+ [Clostridium] scindens VPI 12708 the enzyme participates in the bile acid 7alpha-dehydroxylation pathway 7alpha-hydroxy-3-oxochol-4-en-24-oyl-CoA + NADH + H+
-
 ?
1.3.1.116 7beta-hydroxy-3-oxochol-24-oyl-CoA + NAD+ [Clostridium] scindens
-
 7beta-hydroxy-3-oxochol-4-en-24-oyl-CoA + NADH + H+
-
 ?
1.3.1.116 7beta-hydroxy-3-oxochol-24-oyl-CoA + NAD+ [Clostridium] scindens the enzyme participates in the bile acid 7beta-dehydroxylation pathway 7beta-hydroxy-3-oxochol-4-en-24-oyl-CoA + NADH + H+
-
 ?
1.3.1.116 7beta-hydroxy-3-oxochol-24-oyl-CoA + NAD+ [Clostridium] scindens JCM 10418
-
 7beta-hydroxy-3-oxochol-4-en-24-oyl-CoA + NADH + H+
-
 ?
1.3.1.116 7beta-hydroxy-3-oxochol-24-oyl-CoA + NAD+ [Clostridium] scindens JCM 10418 the enzyme participates in the bile acid 7beta-dehydroxylation pathway 7beta-hydroxy-3-oxochol-4-en-24-oyl-CoA + NADH + H+
-
 ?
1.3.1.116 7beta-hydroxy-3-oxochol-24-oyl-CoA + NAD+ [Clostridium] scindens VPI 12708
-
 7beta-hydroxy-3-oxochol-4-en-24-oyl-CoA + NADH + H+
-
 ?
1.3.1.116 7beta-hydroxy-3-oxochol-24-oyl-CoA + NAD+ [Clostridium] scindens VPI 12708 the enzyme participates in the bile acid 7beta-dehydroxylation pathway 7beta-hydroxy-3-oxochol-4-en-24-oyl-CoA + NADH + H+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.3.1.115 [Clostridium] scindens
-
-
-
1.3.1.115 [Clostridium] scindens P19410
-
-
1.3.1.115 [Clostridium] scindens JCM 10418
-
-
-
1.3.1.115 [Clostridium] scindens VPI 12708 P19410
-
-
1.3.1.116 [Clostridium] scindens P32370
-
-
1.3.1.116 [Clostridium] scindens JCM 10418 P32370
-
-
1.3.1.116 [Clostridium] scindens VPI 12708 P32370
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.3.1.115
-
 [Clostridium] scindens
1.3.1.116
-
 [Clostridium] scindens

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.3.1.116 16.4
-
 37°C, pH 6.8 [Clostridium] scindens
1.3.1.116 16.4
-
 pH 6.8, 25°C [Clostridium] scindens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.1.115 7alpha,12alpha-dihydroxy-3-oxochol-24-oyl-CoA + NAD+
-
 [Clostridium] scindens 7alpha,12alpha-dihydroxy-3-oxochol-4-en-24-oyl-CoA + NADH + H+
-
 ?
1.3.1.115 7alpha,12alpha-dihydroxy-3-oxochol-24-oyl-CoA + NAD+ the enzyme participates in the bile acid 7alpha-dehydroxylation pathway [Clostridium] scindens 7alpha,12alpha-dihydroxy-3-oxochol-4-en-24-oyl-CoA + NADH + H+
-
 ?
1.3.1.115 7alpha,12alpha-dihydroxy-3-oxochol-24-oyl-CoA + NAD+ the enzyme catalyses the stereo-specific oxidation of its substrates and has no activity with the 7beta anomer [Clostridium] scindens 7alpha,12alpha-dihydroxy-3-oxochol-4-en-24-oyl-CoA + NADH + H+
-
 ?
1.3.1.115 7alpha,12alpha-dihydroxy-3-oxochol-24-oyl-CoA + NAD+
-
 [Clostridium] scindens JCM 10418 7alpha,12alpha-dihydroxy-3-oxochol-4-en-24-oyl-CoA + NADH + H+
-
 ?
1.3.1.115 7alpha,12alpha-dihydroxy-3-oxochol-24-oyl-CoA + NAD+ the enzyme participates in the bile acid 7alpha-dehydroxylation pathway [Clostridium] scindens VPI 12708 7alpha,12alpha-dihydroxy-3-oxochol-4-en-24-oyl-CoA + NADH + H+
-
 ?
1.3.1.115 7alpha,12alpha-dihydroxy-3-oxochol-24-oyl-CoA + NAD+ the enzyme catalyses the stereo-specific oxidation of its substrates and has no activity with the 7beta anomer [Clostridium] scindens VPI 12708 7alpha,12alpha-dihydroxy-3-oxochol-4-en-24-oyl-CoA + NADH + H+
-
 ?
1.3.1.115 7alpha-hydroxy-3-oxochol-24-oyl-CoA + NAD+
-
 [Clostridium] scindens 7alpha-hydroxy-3-oxochol-4-en-24-oyl-CoA + NADH + H+
-
 ?
1.3.1.115 7alpha-hydroxy-3-oxochol-24-oyl-CoA + NAD+ the enzyme participates in the bile acid 7alpha-dehydroxylation pathway [Clostridium] scindens 7alpha-hydroxy-3-oxochol-4-en-24-oyl-CoA + NADH + H+
-
 ?
1.3.1.115 7alpha-hydroxy-3-oxochol-24-oyl-CoA + NAD+ the enzyme catalyses the stereo-specific oxidation of its substrates and has no activity with the 7beta anomer [Clostridium] scindens 7alpha-hydroxy-3-oxochol-4-en-24-oyl-CoA + NADH + H+
-
 ?
1.3.1.115 7alpha-hydroxy-3-oxochol-24-oyl-CoA + NAD+
-
 [Clostridium] scindens JCM 10418 7alpha-hydroxy-3-oxochol-4-en-24-oyl-CoA + NADH + H+
-
 ?
1.3.1.115 7alpha-hydroxy-3-oxochol-24-oyl-CoA + NAD+ the enzyme participates in the bile acid 7alpha-dehydroxylation pathway [Clostridium] scindens VPI 12708 7alpha-hydroxy-3-oxochol-4-en-24-oyl-CoA + NADH + H+
-
 ?
1.3.1.115 7alpha-hydroxy-3-oxochol-24-oyl-CoA + NAD+ the enzyme catalyses the stereo-specific oxidation of its substrates and has no activity with the 7beta anomer [Clostridium] scindens VPI 12708 7alpha-hydroxy-3-oxochol-4-en-24-oyl-CoA + NADH + H+
-
 ?
1.3.1.116 7beta-hydroxy-3-oxochol-24-oyl-CoA + NAD+
-
 [Clostridium] scindens 7beta-hydroxy-3-oxochol-4-en-24-oyl-CoA + NADH + H+
-
 ?
1.3.1.116 7beta-hydroxy-3-oxochol-24-oyl-CoA + NAD+ the enzyme participates in the bile acid 7beta-dehydroxylation pathway [Clostridium] scindens 7beta-hydroxy-3-oxochol-4-en-24-oyl-CoA + NADH + H+
-
 ?
1.3.1.116 7beta-hydroxy-3-oxochol-24-oyl-CoA + NAD+ the enzyme catalyses the stereo-specific oxidation of its substrate and has no activity with the 7alpha anomer [Clostridium] scindens 7beta-hydroxy-3-oxochol-4-en-24-oyl-CoA + NADH + H+
-
 ?
1.3.1.116 7beta-hydroxy-3-oxochol-24-oyl-CoA + NAD+
-
 [Clostridium] scindens JCM 10418 7beta-hydroxy-3-oxochol-4-en-24-oyl-CoA + NADH + H+
-
 ?
1.3.1.116 7beta-hydroxy-3-oxochol-24-oyl-CoA + NAD+ the enzyme participates in the bile acid 7beta-dehydroxylation pathway [Clostridium] scindens JCM 10418 7beta-hydroxy-3-oxochol-4-en-24-oyl-CoA + NADH + H+
-
 ?
1.3.1.116 7beta-hydroxy-3-oxochol-24-oyl-CoA + NAD+ the enzyme catalyses the stereo-specific oxidation of its substrate and has no activity with the 7alpha anomer [Clostridium] scindens JCM 10418 7beta-hydroxy-3-oxochol-4-en-24-oyl-CoA + NADH + H+
-
 ?
1.3.1.116 7beta-hydroxy-3-oxochol-24-oyl-CoA + NAD+
-
 [Clostridium] scindens VPI 12708 7beta-hydroxy-3-oxochol-4-en-24-oyl-CoA + NADH + H+
-
 ?
1.3.1.116 7beta-hydroxy-3-oxochol-24-oyl-CoA + NAD+ the enzyme participates in the bile acid 7beta-dehydroxylation pathway [Clostridium] scindens VPI 12708 7beta-hydroxy-3-oxochol-4-en-24-oyl-CoA + NADH + H+
-
 ?
1.3.1.116 7beta-hydroxy-3-oxochol-24-oyl-CoA + NAD+ the enzyme catalyses the stereo-specific oxidation of its substrate and has no activity with the 7alpha anomer [Clostridium] scindens VPI 12708 7beta-hydroxy-3-oxochol-4-en-24-oyl-CoA + NADH + H+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.3.1.115 ? x * 70000, SDS-PAGE [Clostridium] scindens
1.3.1.116 ? x * 72000, SDS-PAGE [Clostridium] scindens
1.3.1.116 homotrimer 3 * 72000, SDS-PAGE [Clostridium] scindens

Synonyms

EC Number Synonyms Comment Organism
1.3.1.115 3-oxo-DELTA4-cholenoic acid oxidoreductase
-
 [Clostridium] scindens
1.3.1.115 BaiCD
-
 [Clostridium] scindens
1.3.1.116 7beta-hydroxy-3-oxo-DELTA4-cholenoic acid oxidoreductase
-
 [Clostridium] scindens
1.3.1.116 BaiH
-
 [Clostridium] scindens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.3.1.115 25
-
 assay at [Clostridium] scindens
1.3.1.116 25
-
 assay at [Clostridium] scindens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.3.1.115 6.8
-
 assay at [Clostridium] scindens
1.3.1.116 6.8
-
 assay at [Clostridium] scindens

Cofactor

EC Number Cofactor Comment Organism Structure
1.3.1.115 FAD
-
 [Clostridium] scindens
1.3.1.116 FAD flavoprotein containing 1 mol of FMN and 1 mol of FAD per subunit [Clostridium] scindens
1.3.1.116 FMN flavoprotein containing 1 mol of FMN and 1 mol of FAD per subunit [Clostridium] scindens
1.3.1.116 additional information no cofactor: NADP+ [Clostridium] scindens
1.3.1.116 NAD+
-
 [Clostridium] scindens
1.3.1.116 NADH
-
 [Clostridium] scindens

General Information

EC Number General Information Comment Organism
1.3.1.115 metabolism the enzyme participates in the bile acid 7alpha-dehydroxylation pathway [Clostridium] scindens
1.3.1.116 metabolism the enzyme participates in the bile acid 7beta-dehydroxylation pathway [Clostridium] scindens