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Literature summary extracted from

  • Baron, S.; Hylemon, P.
    Expression of the bile acid-inducible NADH flavin oxidoreductase gene of Eubacterium sp. VPI 12708 in Escherichia coli (1995), Biochim. Biophys. Acta, 1249, 145-154 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.3.1.116 expression in Escherichia coli [Clostridium] scindens
1.3.1.116 subcloned into an IPTG-inducible expression vector, pBaiH2.2. Escherichia coli DH5a cells transformed with pBaiH2.2 express 10fold higher levels of NADH:FOR upon induction with IPTG than does Eubacterium sp. VPI 12708 cells induced with cholic acid [Clostridium] scindens
1.5.1.36 subcloned into an IPTG-inducible expression vector, pBaiH2.2. Escherichia coli DH5a cells transformed with pBaiH2.2 express 10fold higher levels of NADH:FOR upon induction with IPTG than did Eubacterium sp. VPI 12708 cells induced with cholic acid [Clostridium] scindens

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.3.1.116 1,10-phenanthroline
-
 [Clostridium] scindens
1.3.1.116 Acriflavine
-
 [Clostridium] scindens
1.3.1.116 CuCl2
-
 [Clostridium] scindens
1.3.1.116 EDTA
-
 [Clostridium] scindens
1.3.1.116 EGTA
-
 [Clostridium] scindens
1.3.1.116 HgCl2
-
 [Clostridium] scindens
1.3.1.116 additional information iodoacetate and iodoacetamide show no inhibition [Clostridium] scindens
1.3.1.116 N-bromosuccinimide
-
 [Clostridium] scindens
1.3.1.116 N-ethylmaleimide
-
 [Clostridium] scindens
1.3.1.116 p-chloromercuriphenylsulfonic acid
-
 [Clostridium] scindens
1.3.1.116 Sodium azide
-
 [Clostridium] scindens
1.3.1.116 ZnCl2
-
 [Clostridium] scindens
1.5.1.36 1,10-phenanthroline
-
 [Clostridium] scindens
1.5.1.36 Acriflavine
-
 [Clostridium] scindens
1.5.1.36 CuCl2
-
 [Clostridium] scindens
1.5.1.36 EDTA
-
 [Clostridium] scindens
1.5.1.36 EGTA
-
 [Clostridium] scindens
1.5.1.36 HgCl2
-
 [Clostridium] scindens
1.5.1.36 additional information iodoacetate and iodoacetamide show no inhibition [Clostridium] scindens
1.5.1.36 N-bromosuccinimide
-
 [Clostridium] scindens
1.5.1.36 N-ethylmaleimide
-
 [Clostridium] scindens
1.5.1.36 p-chloromercuriphenylsulfonic acid
-
 [Clostridium] scindens
1.5.1.36 Sodium azide
-
 [Clostridium] scindens
1.5.1.36 ZnCl2
-
 [Clostridium] scindens

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.3.1.116 copper 1 mol of copper per mol of subunit [Clostridium] scindens
1.3.1.116 copper contains 1 mol of copper per subunit [Clostridium] scindens
1.3.1.116 Iron 2 mol of iron per mol of subunit [Clostridium] scindens
1.3.1.116 Iron contains up to 2 mol of iron per subunit [Clostridium] scindens
1.5.1.36 copper contains 1 mol of copper per subunit [Clostridium] scindens
1.5.1.36 Iron contains up to 2 mol of iron per subunit [Clostridium] scindens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.3.1.116 71400
-
 SDS-PAGE [Clostridium] scindens
1.3.1.116 210000
-
 gel filtration [Clostridium] scindens
1.3.1.116 210000
-
 non-denaturing PAGE [Clostridium] scindens
1.5.1.36 210000
-
 non-denaturing PAGE [Clostridium] scindens

Organism

EC Number Organism UniProt Comment Textmining
1.3.1.116 [Clostridium] scindens P32370
-
-
1.3.1.116 [Clostridium] scindens JCM 10418 P32370
-
-
1.3.1.116 [Clostridium] scindens VPI 12708 P32370
-
-
1.5.1.36 [Clostridium] scindens P32370
-
-
1.5.1.36 [Clostridium] scindens VPI 12708 P32370
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.3.1.116
-
 [Clostridium] scindens
1.3.1.116 recombinant protein [Clostridium] scindens
1.5.1.36
-
 [Clostridium] scindens

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.1.116 additional information the enzyme reduces synthetic quinones, dyes, flavins, and O2 with NADH as the electron donor, but does not reduce disulfide compounds, various unsaturated bile acids, cytochrome c and physiological quinones [Clostridium] scindens ?
-
 ?
1.3.1.116 additional information the enzyme reduces synthetic quinones, dyes, flavins, and O2 with NADH as the electron donor, but does not reduce disulfide compounds, various unsaturated bile acids, cytochrome c and physiological quinones [Clostridium] scindens JCM 10418 ?
-
 ?
1.3.1.116 additional information the enzyme reduces synthetic quinones, dyes, flavins, and O2 with NADH as the electron donor, but does not reduce disulfide compounds, various unsaturated bile acids, cytochrome c and physiological quinones [Clostridium] scindens VPI 12708 ?
-
 ?
1.5.1.36 reduced flavin + NAD+
-
 [Clostridium] scindens flavin + NADH + H+
-
 ?
1.5.1.36 reduced flavin + NAD+
-
 [Clostridium] scindens JCM 10418 flavin + NADH + H+
-
 ?
1.5.1.36 reduced flavin + NAD+
-
 [Clostridium] scindens VPI 12708 flavin + NADH + H+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.3.1.116 homotrimer 3 * 71400, SDS-PAGE [Clostridium] scindens
1.3.1.116 trimer 3 * 72000, SDS-PAGE [Clostridium] scindens
1.5.1.36 homotrimer 3 * 71400 [Clostridium] scindens

Synonyms

EC Number Synonyms Comment Organism
1.3.1.116 BaiH
-
 [Clostridium] scindens
1.5.1.36 BaiH
-
 [Clostridium] scindens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.3.1.116 40
-
-
 [Clostridium] scindens
1.5.1.36 40
-
-
 [Clostridium] scindens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.3.1.116 6.8
-
-
 [Clostridium] scindens
1.5.1.36 6.8
-
-
 [Clostridium] scindens

Cofactor

EC Number Cofactor Comment Organism Structure
1.3.1.116 FAD 1 mol of FAD per mol of subunit [Clostridium] scindens
1.3.1.116 FAD contains 1 mol of FAD [Clostridium] scindens
1.3.1.116 NADH
-
 [Clostridium] scindens
1.5.1.36 FAD contains 1 mol of FAD [Clostridium] scindens
1.5.1.36 NADH
-
 [Clostridium] scindens

pI Value

EC Number Organism Comment pI Value Maximum pI Value
1.3.1.116 [Clostridium] scindens isoelectric focusing
-
 6
1.5.1.36 [Clostridium] scindens isoelectric focusing
-
 6

General Information

EC Number General Information Comment Organism
1.3.1.116 physiological function enzyme reduces synthetic quinones, dyes, flavins, and O2 with NADH as the electron donor. Addition of purified enzyme to Clostridium scindens cell extracts alters the balance of oxidized and reduced bile acid intermediates produced during cholic acid 7alpha-dehydroxylation [Clostridium] scindens