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Literature summary extracted from

  • Vashisht, K.; Verma, S.; Gupta, S.; Lynn, A.M.; Dixit, R.; Mishra, N.; Valecha, N.; Hamblin, K.A.; Maytum, R.; Pandey, K.C.; van der Giezen, M.
    Engineering nucleotide specificity of succinyl-CoA synthetase in Blastocystis the emerging role of gatekeeper residues (2017), Biochemistry, 56, 534-542 .
    View publication on PubMedView publication on EuropePMC

Protein Variants

EC Number Protein Variants Comment Organism
6.2.1.4 K46E/K114D the double mutant shows reduced activity with ATP and high specificity towards GTP compared to the wild type enzyme Blastocystis hominis
6.2.1.4 K46E/K114D/V113L/L227F the quadruple mutant shows no activity with ATP and high activity towards GTP compared to the wild type enzyme Blastocystis hominis
6.2.1.4 V113L/L227F the double mutant shows increased activity with ATP compared to the wild type enzyme Blastocystis hominis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.2.1.4 0.082
-
 GTP mutant enzyme K46E/K114D/V113L/L227F, at pH 7.4 and 21°C Blastocystis hominis
6.2.1.4 0.143
-
 GTP mutant enzyme K46E/K114D, at pH 7.4 and 21°C Blastocystis hominis
6.2.1.4 0.145
-
 ATP wild type enzyme, at pH 7.4 and 21°C Blastocystis hominis
6.2.1.4 0.23
-
 ATP mutant enzyme K46E/K114D, at pH 7.4 and 21°C Blastocystis hominis
6.2.1.4 0.265
-
 ATP mutant enzyme V113L/L227F, at pH 7.4 and 21°C Blastocystis hominis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.2.1.4 ATP + succinate + CoA Blastocystis hominis
-
 ADP + phosphate + succinyl-CoA
-
 ?
6.2.1.4 ATP + succinate + CoA Blastocystis hominis NandII
-
 ADP + phosphate + succinyl-CoA
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
6.2.1.4 Blastocystis hominis
-
-
-
6.2.1.4 Blastocystis hominis NandII
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.2.1.4 ATP + succinate + CoA
-
 Blastocystis hominis ADP + phosphate + succinyl-CoA
-
 ?
6.2.1.4 ATP + succinate + CoA
-
 Blastocystis hominis NandII ADP + phosphate + succinyl-CoA
-
 ?
6.2.1.4 GTP + succinate + CoA substrate for mutant enzymes K46E/K114D and K46E/K114D/V113L/L227F Blastocystis hominis GDP + phosphate + succinyl-CoA
-
 ?
6.2.1.4 GTP + succinate + CoA substrate for mutant enzymes K46E/K114D and K46E/K114D/V113L/L227F Blastocystis hominis NandII GDP + phosphate + succinyl-CoA
-
 ?
6.2.1.4 additional information the wild type enzyme has no activity with GTP Blastocystis hominis ?
-
 ?
6.2.1.4 additional information the wild type enzyme has no activity with GTP Blastocystis hominis NandII ?
-
 ?

Subunits

EC Number Subunits Comment Organism
6.2.1.4 ? x * 35000, SDS-PAGE Blastocystis hominis

Synonyms

EC Number Synonyms Comment Organism
6.2.1.4 ATP-specific SCS
-
 Blastocystis hominis
6.2.1.4 SCSalpha
-
 Blastocystis hominis
6.2.1.4 Succinyl-CoA synthetase
-
 Blastocystis hominis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
6.2.1.4 0.0078
-
 GTP mutant enzyme K46E/K114D/V113L/L227F, at pH 7.4 and 21°C Blastocystis hominis
6.2.1.4 0.0139
-
 ATP wild type enzyme, at pH 7.4 and 21°C Blastocystis hominis
6.2.1.4 0.0176
-
 ATP mutant enzyme K46E/K114D, at pH 7.4 and 21°C Blastocystis hominis
6.2.1.4 0.0177
-
 ATP mutant enzyme V113L/L227F, at pH 7.4 and 21°C Blastocystis hominis
6.2.1.4 0.0195
-
 GTP mutant enzyme K46E/K114D, at pH 7.4 and 21°C Blastocystis hominis

Cofactor

EC Number Cofactor Comment Organism Structure
6.2.1.4 ATP
-
 Blastocystis hominis

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
6.2.1.4 0.066
-
 ATP mutant enzyme V113L/L227F, at pH 7.4 and 21°C Blastocystis hominis
6.2.1.4 0.076
-
 ATP mutant enzyme K46E/K114D, at pH 7.4 and 21°C Blastocystis hominis
6.2.1.4 0.096
-
 GTP mutant enzyme K46E/K114D/V113L/L227F, at pH 7.4 and 21°C Blastocystis hominis
6.2.1.4 0.096
-
 ATP wild type enzyme, at pH 7.4 and 21°C Blastocystis hominis
6.2.1.4 0.136
-
 GTP mutant enzyme K46E/K114D, at pH 7.4 and 21°C Blastocystis hominis