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Literature summary extracted from

  • Basom, E.; Manifold, B.; Thielges, M.
    Conformational heterogeneity and the affinity of substrate molecular recognition by cytochrome P450cam (2017), Biochemistry, 56, 3248-3256 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.15.1 recombinant expression of wild-type and mutant enzymes in Escherichia coli strain BL21(DE3) Pseudomonas putida

Protein Variants

EC Number Protein Variants Comment Organism
1.14.15.1 C334A site-directed mutagenesis, the mutation reduces protein aggregation, but has no effect on catalytic activity Pseudomonas putida
1.14.15.1 Y96C/C334A site-directed mutagenesis, substrate recognition and binding compared to the wild-type, conformational selection mechanism Pseudomonas putida
1.14.15.1 Y96F/C334A site-directed mutagenesis, substrate recognition and binding compared to the wild-type, conformational selection mechanism Pseudomonas putida
1.14.15.1 Y96N/C334A site-directed mutagenesis, substrate recognition and binding compared to the wild-type, conformational selection mechanism Pseudomonas putida

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.15.1 additional information
-
 additional information thermodynamics of wild-type and mutant enzymes with different substrates, overview Pseudomonas putida

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.14.15.1 Fe2+ in heme Pseudomonas putida

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.15.1 (+)-camphor + reduced putidaredoxin + O2 Pseudomonas putida
-
 (+)-exo-5-hydroxycamphor + oxidized putidaredoxin + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.15.1 Pseudomonas putida P00183
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.15.1 recombinant wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) by a process including anion exchange chromatography and gel filtration Pseudomonas putida

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.15.1 (+)-camphor + reduced putidaredoxin + O2
-
 Pseudomonas putida (+)-exo-5-hydroxycamphor + oxidized putidaredoxin + H2O
-
 ?
1.14.15.1 camphane + reduced putidaredoxin + O2
-
 Pseudomonas putida ? + oxidized putidaredoxin + H2O
-
 ?
1.14.15.1 isoborneol + reduced putidaredoxin + O2
-
 Pseudomonas putida ? + oxidized putidaredoxin + H2O
-
 ?
1.14.15.1 additional information analysis of the molecular substrate recognition of wild-type and mutant P450cams by infrared (IR) spectroscopy, differing conformational heterogeneity in the active site of the P450cam variants and changes in heterogeneity upon binding of different substrates likely contribute to their variable affinities via a conformational selection mechanism, overview. Although P450cam is relatively specific for camphor, it also recognizes a number of small, camphor-like substrates, albeit with variable affinity. Substrate binding and active site structure, overview Pseudomonas putida ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.14.15.1 cytochrome p450cam
-
 Pseudomonas putida
1.14.15.1 P450cam
-
 Pseudomonas putida

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.15.1 cytochrome P450
-
 Pseudomonas putida
1.14.15.1 heme
-
 Pseudomonas putida
1.14.15.1 putidaredoxin
-
 Pseudomonas putida