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Literature summary extracted from

  • Miles, Z.D.; Myers, W.K.; Kincannon, W.M.; Britt, R.D.; Bandarian, V.
    Biochemical and spectroscopic studies of epoxyqueuosine reductase a novel iron-sulfur cluster- and cobalamin-containing protein involved in the biosynthesis of queuosine (2015), Biochemistry, 54, 4927-4935 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.17.99.6 expressed in Escherichia coli BL21(DE3) cells Bacillus subtilis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.17.99.6 queuosine34 in tRNA + acceptor + H2O Bacillus subtilis
-
 epoxyqueuosine34 in tRNA + reduced acceptor
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.17.99.6 Bacillus subtilis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.17.99.6 StrepTrap column chromatography Bacillus subtilis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.17.99.6 epoxyqueuosine34 in tRNA + reduced methyl viologen
-
 Bacillus subtilis queuosine34 in tRNA + methyl viologen + H2O
-
 ?
1.17.99.6 queuosine34 in tRNA + acceptor + H2O
-
 Bacillus subtilis epoxyqueuosine34 in tRNA + reduced acceptor
-
 ?

Subunits

EC Number Subunits Comment Organism
1.17.99.6 ? x * 48700, calculated from amino acid sequence Bacillus subtilis
1.17.99.6 ? x * 50000. SDS-PAGE Bacillus subtilis

Synonyms

EC Number Synonyms Comment Organism
1.17.99.6 QueG
-
 Bacillus subtilis

Cofactor

EC Number Cofactor Comment Organism Structure
1.17.99.6 Cobalamin
-
 Bacillus subtilis
1.17.99.6 [4Fe-4S]-center two [4Fe-4S] clusters Bacillus subtilis