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Literature summary extracted from

  • Olson, L.; Orsi, R.; Peterson, F.; Parodi, A.; Kim, J.; DAlessio, C.; Dahms, N.
    Crystal structure and functional analyses of the lectin domain of glucosidase II insights into oligomannose recognition (2015), Biochemistry, 54, 4097-4111.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.207 expression in Escherichia coli Schizosaccharomyces pombe

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.2.1.207 mannose 6-phosphate receptor homology domain of GII beta subunit bound to mannose, to 1.6 A resolution. No major difference in the overall fold of ligand-bound and unbound structures, but a repositioning of side chains throughout the binding pocket, including Y372 Schizosaccharomyces pombe

Protein Variants

EC Number Protein Variants Comment Organism
3.2.1.207 additional information mutations of the primary binding pocket residues and adjacent W409, all inhibit the activity of GII both in vitro and in vivo, they do not cause a significant change in the overall fold of the GII beta subunit mannose 6-phosphate receptor homology domain but impact locally the stability of the binding pocket Schizosaccharomyces pombe
3.2.1.207 W409A mutation substantially decreases GII activity Schizosaccharomyces pombe
3.2.1.207 Y372A mutation of Y372 substantially decreases GII activity Schizosaccharomyces pombe
3.2.1.207 Y372F mutation of Y372 substantially decreases GII activity Schizosaccharomyces pombe

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.207 Schizosaccharomyces pombe Q9USH8 glucosidase 2 subunit beta
-
3.2.1.207 Schizosaccharomyces pombe 972 Q9USH8 glucosidase 2 subunit beta
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.207 GlcMan9GlcNAc + H2O
-
 Schizosaccharomyces pombe D-glucose + Man9GlcNAc
-
 ?
3.2.1.207 GlcMan9GlcNAc + H2O
-
 Schizosaccharomyces pombe 972 D-glucose + Man9GlcNAc
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.207 GTB1
-
 Schizosaccharomyces pombe