EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.404 | 2,3,5,6-tetrachlorohydroquinone + NAD+ | Sphingobium chlorophenolicum | the enzyme participates in the degradation of pentachlorophenol | 2,3,5,6-tetrachloro-1,4-benzoquinone + NADH + H+ | - |
? | |
1.14.13.50 | pentachlorophenol + NADPH + H+ + O2 | Sphingobium chlorophenolicum | degradation of pentachlorophenol | 2,3,5,6-tetrachloro-1,4-benzoquinone + NADP+ + chloride + H2O | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.1.1.404 | Sphingobium chlorophenolicum | - |
- |
- |
1.14.13.50 | Sphingobium chlorophenolicum | P42535 | - |
- |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.404 | 2,3,5,6-tetrachlorohydroquinone + NAD+ | - |
Sphingobium chlorophenolicum | 2,3,5,6-tetrachloro-1,4-benzoquinone + NADH + H+ | - |
? | |
1.1.1.404 | 2,3,5,6-tetrachlorohydroquinone + NAD+ | the enzyme participates in the degradation of pentachlorophenol | Sphingobium chlorophenolicum | 2,3,5,6-tetrachloro-1,4-benzoquinone + NADH + H+ | - |
? | |
1.14.13.50 | pentachlorophenol + NADPH + H+ + O2 | degradation of pentachlorophenol | Sphingobium chlorophenolicum | 2,3,5,6-tetrachloro-1,4-benzoquinone + NADP+ + chloride + H2O | - |
? | |
1.14.13.50 | pentachlorophenol + NADPH + H+ + O2 | the enzyme is an unusually slow flavin-dependent monooxygenase (kcat = 0.02/s) that converts pentachlorophenol to a highly reactive product, tetrachlorobenzoquinone. By stopped-flow spectroscopy, it is shown that the steps up to and including formation of tetrachlorobenzoquinone are rapid (5-30/s). Before products can be released from the active site, the strongly oxidizing tetrachlorobenzoquinone abstracts an electron from a donor at the active site, possibly a cysteine residue, resulting in an off-pathway diradical state that only slowly reverts to an intermediate capable of completing the catalytic cycle | Sphingobium chlorophenolicum | 2,3,5,6-tetrachloro-1,4-benzoquinone + NADP+ + chloride + H2O | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.1.1.404 | homotrimer | - |
Sphingobium chlorophenolicum |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.1.1.404 | TCBQ reductase | - |
Sphingobium chlorophenolicum |
1.14.13.50 | PCP hydroxylase | - |
Sphingobium chlorophenolicum |
1.14.13.50 | pentachlorophenol hydroxylase | - |
Sphingobium chlorophenolicum |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.14.13.50 | 0.02 | - |
Pentachlorophenol | pH 7.0, 22°C | Sphingobium chlorophenolicum |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.404 | FMN | the enzyme is a homotrimer containing a flavin and an Fe2S2 cluster in each subunit | Sphingobium chlorophenolicum | |
1.1.1.404 | [2Fe-2S]-center | the enzyme is a homotrimer containing a flavin and an Fe2S2 cluster in each subunit | Sphingobium chlorophenolicum | |
1.14.13.50 | FAD | flavoprotein | Sphingobium chlorophenolicum |
EC Number | General Information | Comment | Organism |
---|---|---|---|
1.1.1.404 | metabolism | the enzyme participates in the degradation of pentachlorophenol | Sphingobium chlorophenolicum |
1.14.13.50 | metabolism | degradation of pentachlorophenol | Sphingobium chlorophenolicum |