Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Branchini, B.R.; Southworth, T.L.; Fontaine, D.M.; Davis, A.L.; Behney, C.E.; Murtiashaw, M.H.
    A Photinus pyralis and Luciola italica chimeric firefly luciferase produces enhanced bioluminescence (2014), Biochemistry, 53, 6287-6289 .
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
1.13.12.7 additional information construction of a chimeric enzyme PpyLit that contains the N-domain of Photinus pyralis luciferase joined to the C-domain of Luciola italica luciferase, the recombinant chimeric enzyme PpyLit shows 1.8fold enhanced flash-height specific activity, 2.0fold enhanced integration-based specific activity, 2.9fold enhanced catalytic efficiency (kcat/Km), and a 1.4fold greater bioluminescence quantum yield compared to the wild-type Photinus pyralis luciferase. A chimeric enzyme with enhanced catalytic properties that are not simply the sum of the contributions of the two luciferases Photinus pyralis
1.13.12.7 additional information construction of a chimeric enzyme PpyLit that contains the N-domain of Photinus pyralis luciferase joined to the C-domain of Luciola italica luciferase, the recombinant chimeric enzyme PpyLit shows 1.8fold enhanced flash-height specific activity, 2.0fold enhanced integration-based specific activity, 2.9fold enhanced catalytic efficiency (kcat/Km), and a 1.4fold greater bioluminescence quantum yield compared to the wild-type Photinus pyralis luciferase. A chimeric enzyme with enhanced catalytic properties that are not simply the sum of the contributions of the two luciferases Luciola italica

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.13.12.7 dehydroluciferin
-
 Photinus pyralis
1.13.12.7 L-AMP a potent luciferase inhibitor Photinus pyralis
1.13.12.7 oxyluciferin
-
 Photinus pyralis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.13.12.7 D-firefly luciferin + O2 + ATP Photinus pyralis
-
 firefly oxyluciferin + CO2 + AMP + diphosphate + hv
-
 ?
1.13.12.7 D-firefly luciferin + O2 + ATP Luciola italica
-
 firefly oxyluciferin + CO2 + AMP + diphosphate + hv
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.13.12.7 Luciola italica Q1AG35
-
-
1.13.12.7 Photinus pyralis P08659
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.13.12.7 D-firefly luciferin + O2 + ATP
-
 Photinus pyralis firefly oxyluciferin + CO2 + AMP + diphosphate + hv
-
 ?
1.13.12.7 D-firefly luciferin + O2 + ATP
-
 Luciola italica firefly oxyluciferin + CO2 + AMP + diphosphate + hv
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.13.12.7 firefly luciferase
-
 Photinus pyralis
1.13.12.7 Luciola italica luciferase
-
 Luciola italica

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.13.12.7 25
-
-
 Photinus pyralis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.13.12.7 7.8
-
-
 Photinus pyralis

Cofactor

EC Number Cofactor Comment Organism Structure
1.13.12.7 ATP
-
 Photinus pyralis
1.13.12.7 ATP
-
 Luciola italica

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.13.12.7 0.0000038
-
 L-AMP pH 7.8, 25°C Photinus pyralis
1.13.12.7 0.0005
-
 oxyluciferin pH 7.8, 25°C Photinus pyralis
1.13.12.7 0.0005
-
 dehydroluciferin pH 7.8, 25°C Photinus pyralis