Characterization of a TET-like aminopeptidase complex from the hyperthermophilic archaeon Pyrococcus horikoshii
Dura, M.A.; Receveur-Brechot, V.; Andrieu, J.P.; Ebel, C.; Schoehn, G.; Roussel, A.; Franzetti, B.; Biochemistry 44, 3477-3486 (2005) 
Data extracted from this reference:
Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
3.4.11.B9
Co2+
activates
Pyrococcus horikoshii
Cloned(Commentary)
EC Number
Cloned (Commentary)
Organism
3.4.11.B9
expression in Escherichia coli
Pyrococcus horikoshii
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
3.4.11.B9
bestatin
-
Pyrococcus horikoshii
3.4.11.B9
EDTA
-
Pyrococcus horikoshii
3.4.11.B9
HEPES
-
Pyrococcus horikoshii
3.4.11.B9
additional information
the enzyme is insensitive to the papain and trypsin inhibitor antipain, to the chymotrypsin inhibitor chymostatin, to the cysteine protease inhibitor E-64, to the serine and cysteine protease inhibitor leupeptin, to the aspartate protease inhibitor pepstatin, and to the serine protease inhibitors Pefabloc SC and aprotinin. Puromycin, an inhibitor of some exopeptidases, has no effect on the activity of PhTET2
Pyrococcus horikoshii
3.4.11.B9
phosphoramidon
weak inhibition
Pyrococcus horikoshii
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.4.11.B9
475000
-
tetrahedral dodecameric complex, sedimentation velocity experiments
Pyrococcus horikoshii
Organism
EC Number
Organism
UniProt
Commentary
Textmining
3.4.11.B9
Pyrococcus horikoshii
O59196
-
-
3.4.11.B9
Pyrococcus horikoshii OT-3
O59196
-
-
Purification (Commentary)
EC Number
Purification (Commentary)
Organism
3.4.11.B9
-
Pyrococcus horikoshii
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
3.4.11.B9
Ala-Ala 4-nitroanilide + H2O
2.9% of the activity with Leu-4-nitroanilide
744302
Pyrococcus horikoshii
Ala-Ala + 4-nitroaniline
-
-
-
?
3.4.11.B9
Ala-Ala 4-nitroanilide + H2O
2.9% of the activity with Leu-4-nitroanilide
744302
Pyrococcus horikoshii OT-3
Ala-Ala + 4-nitroaniline
-
-
-
?
3.4.11.B9
Ala-Ala-Ala 4-nitroanilide + H2O
1.3% of the activity with Leu-4-nitroanilide
744302
Pyrococcus horikoshii
Ala-Ala-Ala + 4-nitroaniline
-
-
-
?
3.4.11.B9
Ala-Ala-Phe 4-nitroanilide + H2O
1.2% of the activity with Leu-4-nitroanilide
744302
Pyrococcus horikoshii
Ala-Ala-Phe + 4-nitroaniline
-
-
-
?
3.4.11.B9
Asn-7-amido-4-methylcoumarin + H2O
0.3% of the activity with Leu-4-nitroanilide
744302
Pyrococcus horikoshii
Asn + 7-amino-4-methylcoumarin
-
-
-
?
3.4.11.B9
Gln-7-amido-4-methylcoumarin + H2O
2.8% of the activity with Leu-4-nitroanilide
744302
Pyrococcus horikoshii
Gln + 7-amino-4-methylcoumarin
-
-
-
?
3.4.11.B9
Gln-7-amido-4-methylcoumarin + H2O
2.8% of the activity with Leu-4-nitroanilide
744302
Pyrococcus horikoshii OT-3
Gln + 7-amino-4-methylcoumarin
-
-
-
?
3.4.11.B9
glycyl 4-nitroanilide + H2O
0.3% of the activity with Leu-4-nitroanilide
744302
Pyrococcus horikoshii
glycine + 4-nitroaniline
-
-
-
?
3.4.11.B9
L-alanyl 4-nitroanilide + H2O
14.3% of the activity with Leu-4-nitroanilide
744302
Pyrococcus horikoshii
L-alanine + 4-nitroaniline
-
-
-
?
3.4.11.B9
L-arginyl 4-nitroanilide + H2O
0.1% of the activity with Leu-4-nitroanilide
744302
Pyrococcus horikoshii
L-arginine + 4-nitroaniline
-
-
-
?
3.4.11.B9
L-isoleucyl 4-nitroanilide + H2O
10.8% of the activity with Leu-4-nitroanilide
744302
Pyrococcus horikoshii
L-isoleucine + 4-nitroaniline
-
-
-
?
3.4.11.B9
L-leucyl 4-nitroanilide + H2O
-
744302
Pyrococcus horikoshii
L-leucine + 4-nitroaniline
-
-
-
?
3.4.11.B9
L-lysyl 4-nitroanilide + H2O
0.2% of the activity with Leu-4-nitroanilide
744302
Pyrococcus horikoshii
L-lysine + 4-nitroaniline
-
-
-
?
3.4.11.B9
L-methionyl 4-nitroanilide + H2O
26.3% of the activity with Leu-4-nitroanilide
744302
Pyrococcus horikoshii
L-methionine + 4-nitroaniline
-
-
-
?
3.4.11.B9
L-phenylalanyl 4-nitroanilide + H2O
0.8% of the activity with Leu-4-nitroanilide
744302
Pyrococcus horikoshii
L-phenylalanine + 4-nitroaniline
-
-
-
?
3.4.11.B9
L-prolyl 4-nitroanilide + H2O
0.9% of the activity with Leu-4-nitroanilide
744302
Pyrococcus horikoshii
L-proline + 4-nitroaniline
-
-
-
?
3.4.11.B9
additional information
when assayed with short chromogenic peptides, PhTET2 is active as an aminopeptidase, with a clear preference for leucine as the N-terminal amino acid. The enzyme can cleave moderately long polypeptide substrates of various compositions in a fairly unspecific manner. The hydrolytic mechanism is found to be nonprocessive. The enzyme has neither carboxypeptidase nor endoproteolytic activities, and it is devoid of N-terminal deblocking activity
744302
Pyrococcus horikoshii
?
-
-
-
?
3.4.11.B9
additional information
when assayed with short chromogenic peptides, PhTET2 is active as an aminopeptidase, with a clear preference for leucine as the N-terminal amino acid. The enzyme can cleave moderately long polypeptide substrates of various compositions in a fairly unspecific manner. The hydrolytic mechanism is found to be nonprocessive. The enzyme has neither carboxypeptidase nor endoproteolytic activities, and it is devoid of N-terminal deblocking activity
744302
Pyrococcus horikoshii OT-3
?
-
-
-
?
3.4.11.B9
Orn-7-amido-4-methylcoumarin + H2O
0.1% of the activity with Leu-4-nitroanilide
744302
Pyrococcus horikoshii
Orn + 7-amino-4-methylcoumarin
-
-
-
?
3.4.11.B9
Ser-7-amido-4-methylcoumarin + H2O
12.8% of the activity with Leu-4-nitroanilide
744302
Pyrococcus horikoshii
Ser + 7-amino-4-methylcoumarin
-
-
-
?
3.4.11.B9
Ser-7-amido-4-methylcoumarin + H2O
12.8% of the activity with Leu-4-nitroanilide
744302
Pyrococcus horikoshii OT-3
Ser + 7-amino-4-methylcoumarin
-
-
-
?
3.4.11.B9
Thr-7-amido-4-methylcoumarin + H2O
6.1% of the activity with Leu-4-nitroanilide
744302
Pyrococcus horikoshii
Thr + 7-amino-4-methylcoumarin
-
-
-
?
3.4.11.B9
Tyr-7-amido-4-methylcoumarin + H2O
0.9% of the activity with Leu-4-nitroanilide
744302
Pyrococcus horikoshii
Tyr + 7-amino-4-methylcoumarin
-
-
-
?
3.4.11.B9
Tyr-7-amido-4-methylcoumarin + H2O
0.9% of the activity with Leu-4-nitroanilide
744302
Pyrococcus horikoshii OT-3
Tyr + 7-amino-4-methylcoumarin
-
-
-
?
3.4.11.B9
Val-7-amido-4-methylcoumarin + H2O
2.1% of the activity with Leu-4-nitroanilide
744302
Pyrococcus horikoshii
Val + 7-amino-4-methylcoumarin
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
3.4.11.B9
dodecamer
12 * 39014, calculated from sequence
Pyrococcus horikoshii
Synonyms
EC Number
Synonyms
Commentary
Organism
3.4.11.B9
PH1527
locus name
Pyrococcus horikoshii
3.4.11.B9
PhTET2
-
Pyrococcus horikoshii
3.4.11.B9
tetrahedral aminopeptidase 2
-
Pyrococcus horikoshii
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
3.4.11.B9
100
-
-
Pyrococcus horikoshii
Temperature Range [°C]
EC Number
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
3.4.11.B9
70
-
at temperatures lower than 70 °C, less than 10% of the maximum activity was detected
Pyrococcus horikoshii
Temperature Stability [°C]
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
3.4.11.B9
80
-
half-life: 10.03 h
Pyrococcus horikoshii
3.4.11.B9
90
-
half-life: 3.00 h
Pyrococcus horikoshii
3.4.11.B9
95
-
half-life: 59.4 min
Pyrococcus horikoshii
3.4.11.B9
100
-
half-life: 24.8 min
Pyrococcus horikoshii
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.4.11.B9
7.5
-
substrate: Leu-4-nitroanilide, PIPES buffer
Pyrococcus horikoshii
pH Range
EC Number
pH Minimum
pH Maximum
Commentary
Organism
3.4.11.B9
6
9
substrate: Leu-4-nitroanilide, PIPES buffer, strong activity was still detectable at pH 6 and 9
Pyrococcus horikoshii
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
3.4.11.B9
Co2+
activates
Pyrococcus horikoshii
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
3.4.11.B9
expression in Escherichia coli
Pyrococcus horikoshii
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
3.4.11.B9
bestatin
-
Pyrococcus horikoshii
3.4.11.B9
EDTA
-
Pyrococcus horikoshii
3.4.11.B9
HEPES
-
Pyrococcus horikoshii
3.4.11.B9
additional information
the enzyme is insensitive to the papain and trypsin inhibitor antipain, to the chymotrypsin inhibitor chymostatin, to the cysteine protease inhibitor E-64, to the serine and cysteine protease inhibitor leupeptin, to the aspartate protease inhibitor pepstatin, and to the serine protease inhibitors Pefabloc SC and aprotinin. Puromycin, an inhibitor of some exopeptidases, has no effect on the activity of PhTET2
Pyrococcus horikoshii
3.4.11.B9
phosphoramidon
weak inhibition
Pyrococcus horikoshii
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.4.11.B9
475000
-
tetrahedral dodecameric complex, sedimentation velocity experiments
Pyrococcus horikoshii
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
3.4.11.B9
-
Pyrococcus horikoshii
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
3.4.11.B9
Ala-Ala 4-nitroanilide + H2O
2.9% of the activity with Leu-4-nitroanilide
744302
Pyrococcus horikoshii
Ala-Ala + 4-nitroaniline
-
-
-
?
3.4.11.B9
Ala-Ala 4-nitroanilide + H2O
2.9% of the activity with Leu-4-nitroanilide
744302
Pyrococcus horikoshii OT-3
Ala-Ala + 4-nitroaniline
-
-
-
?
3.4.11.B9
Ala-Ala-Ala 4-nitroanilide + H2O
1.3% of the activity with Leu-4-nitroanilide
744302
Pyrococcus horikoshii
Ala-Ala-Ala + 4-nitroaniline
-
-
-
?
3.4.11.B9
Ala-Ala-Phe 4-nitroanilide + H2O
1.2% of the activity with Leu-4-nitroanilide
744302
Pyrococcus horikoshii
Ala-Ala-Phe + 4-nitroaniline
-
-
-
?
3.4.11.B9
Asn-7-amido-4-methylcoumarin + H2O
0.3% of the activity with Leu-4-nitroanilide
744302
Pyrococcus horikoshii
Asn + 7-amino-4-methylcoumarin
-
-
-
?
3.4.11.B9
Gln-7-amido-4-methylcoumarin + H2O
2.8% of the activity with Leu-4-nitroanilide
744302
Pyrococcus horikoshii
Gln + 7-amino-4-methylcoumarin
-
-
-
?
3.4.11.B9
Gln-7-amido-4-methylcoumarin + H2O
2.8% of the activity with Leu-4-nitroanilide
744302
Pyrococcus horikoshii OT-3
Gln + 7-amino-4-methylcoumarin
-
-
-
?
3.4.11.B9
glycyl 4-nitroanilide + H2O
0.3% of the activity with Leu-4-nitroanilide
744302
Pyrococcus horikoshii
glycine + 4-nitroaniline
-
-
-
?
3.4.11.B9
L-alanyl 4-nitroanilide + H2O
14.3% of the activity with Leu-4-nitroanilide
744302
Pyrococcus horikoshii
L-alanine + 4-nitroaniline
-
-
-
?
3.4.11.B9
L-arginyl 4-nitroanilide + H2O
0.1% of the activity with Leu-4-nitroanilide
744302
Pyrococcus horikoshii
L-arginine + 4-nitroaniline
-
-
-
?
3.4.11.B9
L-isoleucyl 4-nitroanilide + H2O
10.8% of the activity with Leu-4-nitroanilide
744302
Pyrococcus horikoshii
L-isoleucine + 4-nitroaniline
-
-
-
?
3.4.11.B9
L-leucyl 4-nitroanilide + H2O
-
744302
Pyrococcus horikoshii
L-leucine + 4-nitroaniline
-
-
-
?
3.4.11.B9
L-lysyl 4-nitroanilide + H2O
0.2% of the activity with Leu-4-nitroanilide
744302
Pyrococcus horikoshii
L-lysine + 4-nitroaniline
-
-
-
?
3.4.11.B9
L-methionyl 4-nitroanilide + H2O
26.3% of the activity with Leu-4-nitroanilide
744302
Pyrococcus horikoshii
L-methionine + 4-nitroaniline
-
-
-
?
3.4.11.B9
L-phenylalanyl 4-nitroanilide + H2O
0.8% of the activity with Leu-4-nitroanilide
744302
Pyrococcus horikoshii
L-phenylalanine + 4-nitroaniline
-
-
-
?
3.4.11.B9
L-prolyl 4-nitroanilide + H2O
0.9% of the activity with Leu-4-nitroanilide
744302
Pyrococcus horikoshii
L-proline + 4-nitroaniline
-
-
-
?
3.4.11.B9
additional information
when assayed with short chromogenic peptides, PhTET2 is active as an aminopeptidase, with a clear preference for leucine as the N-terminal amino acid. The enzyme can cleave moderately long polypeptide substrates of various compositions in a fairly unspecific manner. The hydrolytic mechanism is found to be nonprocessive. The enzyme has neither carboxypeptidase nor endoproteolytic activities, and it is devoid of N-terminal deblocking activity
744302
Pyrococcus horikoshii
?
-
-
-
?
3.4.11.B9
additional information
when assayed with short chromogenic peptides, PhTET2 is active as an aminopeptidase, with a clear preference for leucine as the N-terminal amino acid. The enzyme can cleave moderately long polypeptide substrates of various compositions in a fairly unspecific manner. The hydrolytic mechanism is found to be nonprocessive. The enzyme has neither carboxypeptidase nor endoproteolytic activities, and it is devoid of N-terminal deblocking activity
744302
Pyrococcus horikoshii OT-3
?
-
-
-
?
3.4.11.B9
Orn-7-amido-4-methylcoumarin + H2O
0.1% of the activity with Leu-4-nitroanilide
744302
Pyrococcus horikoshii
Orn + 7-amino-4-methylcoumarin
-
-
-
?
3.4.11.B9
Ser-7-amido-4-methylcoumarin + H2O
12.8% of the activity with Leu-4-nitroanilide
744302
Pyrococcus horikoshii
Ser + 7-amino-4-methylcoumarin
-
-
-
?
3.4.11.B9
Ser-7-amido-4-methylcoumarin + H2O
12.8% of the activity with Leu-4-nitroanilide
744302
Pyrococcus horikoshii OT-3
Ser + 7-amino-4-methylcoumarin
-
-
-
?
3.4.11.B9
Thr-7-amido-4-methylcoumarin + H2O
6.1% of the activity with Leu-4-nitroanilide
744302
Pyrococcus horikoshii
Thr + 7-amino-4-methylcoumarin
-
-
-
?
3.4.11.B9
Tyr-7-amido-4-methylcoumarin + H2O
0.9% of the activity with Leu-4-nitroanilide
744302
Pyrococcus horikoshii
Tyr + 7-amino-4-methylcoumarin
-
-
-
?
3.4.11.B9
Tyr-7-amido-4-methylcoumarin + H2O
0.9% of the activity with Leu-4-nitroanilide
744302
Pyrococcus horikoshii OT-3
Tyr + 7-amino-4-methylcoumarin
-
-
-
?
3.4.11.B9
Val-7-amido-4-methylcoumarin + H2O
2.1% of the activity with Leu-4-nitroanilide
744302
Pyrococcus horikoshii
Val + 7-amino-4-methylcoumarin
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
3.4.11.B9
dodecamer
12 * 39014, calculated from sequence
Pyrococcus horikoshii
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
3.4.11.B9
100
-
-
Pyrococcus horikoshii
Temperature Range [°C] (protein specific)
EC Number
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
3.4.11.B9
70
-
at temperatures lower than 70 °C, less than 10% of the maximum activity was detected
Pyrococcus horikoshii
Temperature Stability [°C] (protein specific)
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
3.4.11.B9
80
-
half-life: 10.03 h
Pyrococcus horikoshii
3.4.11.B9
90
-
half-life: 3.00 h
Pyrococcus horikoshii
3.4.11.B9
95
-
half-life: 59.4 min
Pyrococcus horikoshii
3.4.11.B9
100
-
half-life: 24.8 min
Pyrococcus horikoshii
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.4.11.B9
7.5
-
substrate: Leu-4-nitroanilide, PIPES buffer
Pyrococcus horikoshii
pH Range (protein specific)
EC Number
pH Minimum
pH Maximum
Commentary
Organism
3.4.11.B9
6
9
substrate: Leu-4-nitroanilide, PIPES buffer, strong activity was still detectable at pH 6 and 9
Pyrococcus horikoshii