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Literature summary extracted from

  • Dura, M.A.; Receveur-Brechot, V.; Andrieu, J.P.; Ebel, C.; Schoehn, G.; Roussel, A.; Franzetti, B.
    Characterization of a TET-like aminopeptidase complex from the hyperthermophilic archaeon Pyrococcus horikoshii (2005), Biochemistry, 44, 3477-3486 .
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.4.11.B9 Co2+ activates Pyrococcus horikoshii

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.11.B9 expression in Escherichia coli Pyrococcus horikoshii

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.11.B9 bestatin
-
 Pyrococcus horikoshii
3.4.11.B9 EDTA
-
 Pyrococcus horikoshii
3.4.11.B9 HEPES
-
 Pyrococcus horikoshii
3.4.11.B9 additional information the enzyme is insensitive to the papain and trypsin inhibitor antipain, to the chymotrypsin inhibitor chymostatin, to the cysteine protease inhibitor E-64, to the serine and cysteine protease inhibitor leupeptin, to the aspartate protease inhibitor pepstatin, and to the serine protease inhibitors Pefabloc SC and aprotinin. Puromycin, an inhibitor of some exopeptidases, has no effect on the activity of PhTET2 Pyrococcus horikoshii
3.4.11.B9 phosphoramidon weak inhibition Pyrococcus horikoshii

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.11.B9 475000
-
 tetrahedral dodecameric complex, sedimentation velocity experiments Pyrococcus horikoshii

Organism

EC Number Organism UniProt Comment Textmining
3.4.11.B9 Pyrococcus horikoshii O59196
-
-
3.4.11.B9 Pyrococcus horikoshii OT-3 O59196
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.11.B9
-
 Pyrococcus horikoshii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.11.B9 Ala-Ala 4-nitroanilide + H2O 2.9% of the activity with Leu-4-nitroanilide Pyrococcus horikoshii Ala-Ala + 4-nitroaniline
-
 ?
3.4.11.B9 Ala-Ala 4-nitroanilide + H2O 2.9% of the activity with Leu-4-nitroanilide Pyrococcus horikoshii OT-3 Ala-Ala + 4-nitroaniline
-
 ?
3.4.11.B9 Ala-Ala-Ala 4-nitroanilide + H2O 1.3% of the activity with Leu-4-nitroanilide Pyrococcus horikoshii Ala-Ala-Ala + 4-nitroaniline
-
 ?
3.4.11.B9 Ala-Ala-Phe 4-nitroanilide + H2O 1.2% of the activity with Leu-4-nitroanilide Pyrococcus horikoshii Ala-Ala-Phe + 4-nitroaniline
-
 ?
3.4.11.B9 Asn-7-amido-4-methylcoumarin + H2O 0.3% of the activity with Leu-4-nitroanilide Pyrococcus horikoshii Asn + 7-amino-4-methylcoumarin
-
 ?
3.4.11.B9 Gln-7-amido-4-methylcoumarin + H2O 2.8% of the activity with Leu-4-nitroanilide Pyrococcus horikoshii Gln + 7-amino-4-methylcoumarin
-
 ?
3.4.11.B9 Gln-7-amido-4-methylcoumarin + H2O 2.8% of the activity with Leu-4-nitroanilide Pyrococcus horikoshii OT-3 Gln + 7-amino-4-methylcoumarin
-
 ?
3.4.11.B9 glycyl 4-nitroanilide + H2O 0.3% of the activity with Leu-4-nitroanilide Pyrococcus horikoshii glycine + 4-nitroaniline
-
 ?
3.4.11.B9 L-alanyl 4-nitroanilide + H2O 14.3% of the activity with Leu-4-nitroanilide Pyrococcus horikoshii L-alanine + 4-nitroaniline
-
 ?
3.4.11.B9 L-arginyl 4-nitroanilide + H2O 0.1% of the activity with Leu-4-nitroanilide Pyrococcus horikoshii L-arginine + 4-nitroaniline
-
 ?
3.4.11.B9 L-isoleucyl 4-nitroanilide + H2O 10.8% of the activity with Leu-4-nitroanilide Pyrococcus horikoshii L-isoleucine + 4-nitroaniline
-
 ?
3.4.11.B9 L-leucyl 4-nitroanilide + H2O
-
 Pyrococcus horikoshii L-leucine + 4-nitroaniline
-
 ?
3.4.11.B9 L-lysyl 4-nitroanilide + H2O 0.2% of the activity with Leu-4-nitroanilide Pyrococcus horikoshii L-lysine + 4-nitroaniline
-
 ?
3.4.11.B9 L-methionyl 4-nitroanilide + H2O 26.3% of the activity with Leu-4-nitroanilide Pyrococcus horikoshii L-methionine + 4-nitroaniline
-
 ?
3.4.11.B9 L-phenylalanyl 4-nitroanilide + H2O 0.8% of the activity with Leu-4-nitroanilide Pyrococcus horikoshii L-phenylalanine + 4-nitroaniline
-
 ?
3.4.11.B9 L-prolyl 4-nitroanilide + H2O 0.9% of the activity with Leu-4-nitroanilide Pyrococcus horikoshii L-proline + 4-nitroaniline
-
 ?
3.4.11.B9 additional information when assayed with short chromogenic peptides, PhTET2 is active as an aminopeptidase, with a clear preference for leucine as the N-terminal amino acid. The enzyme can cleave moderately long polypeptide substrates of various compositions in a fairly unspecific manner. The hydrolytic mechanism is found to be nonprocessive. The enzyme has neither carboxypeptidase nor endoproteolytic activities, and it is devoid of N-terminal deblocking activity Pyrococcus horikoshii ?
-
 ?
3.4.11.B9 additional information when assayed with short chromogenic peptides, PhTET2 is active as an aminopeptidase, with a clear preference for leucine as the N-terminal amino acid. The enzyme can cleave moderately long polypeptide substrates of various compositions in a fairly unspecific manner. The hydrolytic mechanism is found to be nonprocessive. The enzyme has neither carboxypeptidase nor endoproteolytic activities, and it is devoid of N-terminal deblocking activity Pyrococcus horikoshii OT-3 ?
-
 ?
3.4.11.B9 Orn-7-amido-4-methylcoumarin + H2O 0.1% of the activity with Leu-4-nitroanilide Pyrococcus horikoshii Orn + 7-amino-4-methylcoumarin
-
 ?
3.4.11.B9 Ser-7-amido-4-methylcoumarin + H2O 12.8% of the activity with Leu-4-nitroanilide Pyrococcus horikoshii Ser + 7-amino-4-methylcoumarin
-
 ?
3.4.11.B9 Ser-7-amido-4-methylcoumarin + H2O 12.8% of the activity with Leu-4-nitroanilide Pyrococcus horikoshii OT-3 Ser + 7-amino-4-methylcoumarin
-
 ?
3.4.11.B9 Thr-7-amido-4-methylcoumarin + H2O 6.1% of the activity with Leu-4-nitroanilide Pyrococcus horikoshii Thr + 7-amino-4-methylcoumarin
-
 ?
3.4.11.B9 Tyr-7-amido-4-methylcoumarin + H2O 0.9% of the activity with Leu-4-nitroanilide Pyrococcus horikoshii Tyr + 7-amino-4-methylcoumarin
-
 ?
3.4.11.B9 Tyr-7-amido-4-methylcoumarin + H2O 0.9% of the activity with Leu-4-nitroanilide Pyrococcus horikoshii OT-3 Tyr + 7-amino-4-methylcoumarin
-
 ?
3.4.11.B9 Val-7-amido-4-methylcoumarin + H2O 2.1% of the activity with Leu-4-nitroanilide Pyrococcus horikoshii Val + 7-amino-4-methylcoumarin
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.11.B9 dodecamer 12 * 39014, calculated from sequence Pyrococcus horikoshii

Synonyms

EC Number Synonyms Comment Organism
3.4.11.B9 PH1527 locus name Pyrococcus horikoshii
3.4.11.B9 PhTET2
-
 Pyrococcus horikoshii
3.4.11.B9 tetrahedral aminopeptidase 2
-
 Pyrococcus horikoshii

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.11.B9 100
-
-
 Pyrococcus horikoshii

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.4.11.B9 70
-
 at temperatures lower than 70 °C, less than 10% of the maximum activity was detected Pyrococcus horikoshii

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.4.11.B9 80
-
 half-life: 10.03 h Pyrococcus horikoshii
3.4.11.B9 90
-
 half-life: 3.00 h Pyrococcus horikoshii
3.4.11.B9 95
-
 half-life: 59.4 min Pyrococcus horikoshii
3.4.11.B9 100
-
 half-life: 24.8 min Pyrococcus horikoshii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.11.B9 7.5
-
 substrate: Leu-4-nitroanilide, PIPES buffer Pyrococcus horikoshii

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.4.11.B9 6 9 substrate: Leu-4-nitroanilide, PIPES buffer, strong activity was still detectable at pH 6 and 9 Pyrococcus horikoshii