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Literature summary extracted from

  • Wang, P.; Xia, Y.; Li, J.; Kang, Z.; Zhou, J.; Chen, J.
    Overexpression of pyrroloquinoline quinone biosynthetic genes affects L-sorbose production in Gluconobacter oxydans WSH-003 (2016), Biochem. Eng. J., 112, 70-77 .
No PubMed abstract available

Application

EC Number Application Comment Organism
1.3.3.11 biotechnology cofactor engineering of PQQ in Gluconobacter oxydans is beneficial for enhancing the production of quinoprotein-related products Gluconobacter oxydans
1.3.3.11 synthesis cofactor engineering of PQQ in Gluconobacter oxydans is beneficial for enhancing the production of quinoprotein-related products Gluconobacter oxydans

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.3.3.11 gene pqqC, enzymes that biosynthesize PQQ are encoded by the pqqABCDE gene cluster and the tldD gene. The tufB promoter is ligated withpqqA, pqqB, pqqC, pqqD, pqqE and tldD, and the pqqA promoter is ligated with pqqB, pqqC, pqqD and pqqE, respectively, through fusionPCR, subcloning in Escherichia coli. Overexpression Gluconobacter oxydans strain WSH-003 of pqqA, pqqB, pqqC, pqqD and pqqE with the pqqA promoter enhances the extracellular PQQ concentration by 38.5%, 68.4%, 19.9%, 30.3% and 8.2%, respectively, whereas production is enhanced by 59.4%, 85.7%, 30.9%, 42.2%, and 19.3%, respectively, using the tufB promoter. Metabolic effects, overview Gluconobacter oxydans

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.3.3.11 6-(2-amino-2-carboxyethyl)-7,8-dioxo-1,2,3,4,7,8-hexahydroquinoline-2,4-dicarboxylate + 3 O2 Gluconobacter oxydans
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 4,5-dioxo-1H-pyrrolo[2,3-f]quinoline-2,7,9-tricarboxylate + 2 H2O2 + 2 H2O
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 ?
1.3.3.11 6-(2-amino-2-carboxyethyl)-7,8-dioxo-1,2,3,4,7,8-hexahydroquinoline-2,4-dicarboxylate + 3 O2 Gluconobacter oxydans WSH-003
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 4,5-dioxo-1H-pyrrolo[2,3-f]quinoline-2,7,9-tricarboxylate + 2 H2O2 + 2 H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.3.3.11 Gluconobacter oxydans Q9L3B2
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1.3.3.11 Gluconobacter oxydans WSH-003 Q9L3B2
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-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.3.11 6-(2-amino-2-carboxyethyl)-7,8-dioxo-1,2,3,4,7,8-hexahydroquinoline-2,4-dicarboxylate + 3 O2
-
 Gluconobacter oxydans 4,5-dioxo-1H-pyrrolo[2,3-f]quinoline-2,7,9-tricarboxylate + 2 H2O2 + 2 H2O
-
 ?
1.3.3.11 6-(2-amino-2-carboxyethyl)-7,8-dioxo-1,2,3,4,7,8-hexahydroquinoline-2,4-dicarboxylate + 3 O2
-
 Gluconobacter oxydans WSH-003 4,5-dioxo-1H-pyrrolo[2,3-f]quinoline-2,7,9-tricarboxylate + 2 H2O2 + 2 H2O
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.3.3.11 PqqC
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 Gluconobacter oxydans

General Information

EC Number General Information Comment Organism
1.3.3.11 physiological function the enzyme is involved in biosynthesis of pyrroloquinoline quinone, PQQ, a cofactor of various membrane-bound dehydrogenases. The amount of endogenous PQQ is generally regarded as a bottleneck to achieving higher catalytic efficiency of PQQ-dependent dehydrogenases. PQQ biosynthesis can be enhanced by overexpressing some of the individual genes in the gene cluster in Gluconobacter oxydans and the PQQ levels are positively correlated with the efficiency of conversion of D-sorbitol to L-sorbose, overexpression of PQQ-related genes activates L-sorbose production and D-sorbitol dehydrogenase activity Gluconobacter oxydans