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Literature summary extracted from

  • Ma, Y.; Ma, X.H.; Meng, F.Y.; Zhan, Z.L.; Guo, J.; Huang, L.Q.
    RNA interference targeting CYP76AH1 in hairy roots of Salvia miltiorrhiza reveals its key role in the biosynthetic pathway of tanshinones (2016), Biochem. Biophys. Res. Commun., 477, 155-160 .
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
1.14.14.175 additional information RNAi-mediated silencing of CYP76AH1 in hairy roots, construction of a CYP76AH1 RNAi system for hairy roots using transformation via Agrobacterium rhizogenes strain C58C1. Metabolic analysis of RNAi-AH1 hairy root lines shows a significantly increased accumulation of miltiradiene and decreased concentration of ferruginol compared to the control lines. The content of tanshinones decreases significantly after silencing of CYP76AH1 Salvia miltiorrhiza

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.14.175 abieta-8,11,13-triene + [reduced NADPH-hemoprotein reductase] + O2 Salvia miltiorrhiza
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ferruginol + [oxidized NADPH-hemoprotein reductase] + H2O
-
?

Organism

EC Number Organism UniProt Comment Textmining
1.14.14.175 Salvia miltiorrhiza S4UX02
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.14.14.175 hairy root
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Salvia miltiorrhiza
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Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.14.175 abieta-8,11,13-triene + [reduced NADPH-hemoprotein reductase] + O2
-
Salvia miltiorrhiza ferruginol + [oxidized NADPH-hemoprotein reductase] + H2O
-
?
1.14.14.175 abieta-8,11,13-triene + [reduced NADPH-hemoprotein reductase] + O2 i.e. miltiradiene Salvia miltiorrhiza ferruginol + [oxidized NADPH-hemoprotein reductase] + H2O
-
?

Synonyms

EC Number Synonyms Comment Organism
1.14.14.175 CYP76AH1
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Salvia miltiorrhiza

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.14.175 cytochrome P-450
-
Salvia miltiorrhiza
1.14.14.175 NADPH
-
Salvia miltiorrhiza

General Information

EC Number General Information Comment Organism
1.14.14.175 metabolism the enzyme catalyzes the first committed step in the biosynthesis of tanshinones, pathay overview Salvia miltiorrhiza
1.14.14.175 physiological function CYP76AH1 catalyzes the conversion of the carbon skeleton miltiradiene into the intermediate ferruginol, which is involved in the biosynthesis of tanshinones, the chief bioactive ingredients of Salvia miltiorrhiza Salvia miltiorrhiza