EC Number | Cloned (Comment) | Organism |
---|---|---|
1.13.12.13 | recombinant expression of His-tagged 19 kDa catalytic subunit mutant nanoKAZ in Escherichia coli strain BL21. In bacterial cells, wild-type KAZ is mainly expressed as inclusion bodies | Oplophorus gracilirostris |
EC Number | Crystallization (Comment) | Organism |
---|---|---|
1.13.12.13 | purified mutated 19 kDa protein nanoKAZ, X-ray diffraction structure determination and analysis at 1.71-2.3.A resolution and dialysis | Oplophorus gracilirostris |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
1.13.12.13 | A4E/Q11R/Q18L/L27V/A33N/K43R/V44I/A54I/F68D/L72Q/M75K/I90V/P115E/Q124K/Y138I/N166R | construction of mutant nanoKAZ, a mutant with 16 amino acid substitutions of the catalytic subunit, by site-directed mutagenesis, the mutant nanoKAZ lacks the amino-terminal signal peptide, crystal structure determination and analysis, structure-function relationship in nanoKAZ, overview. The truncation of 10 amino acid residues at N- and C-terminal regions of nanoKAZ causes a complete loss of luminescence activity. Both the alpha1-helix and beta11-strand in the nanoKAZ molecule might serve to stabilize the molecule, which is essential in catalyzing the luminescence reaction | Oplophorus gracilirostris |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
1.13.12.13 | extracellular | the enzyme possesses an amino-terminal signal peptide and is secreted | Oplophorus gracilirostris | - |
- |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.13.12.13 | 106000 | - |
full-length enzyme | Oplophorus gracilirostris |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.13.12.13 | coelenterazine + O2 | Oplophorus gracilirostris | - |
coelenteramide + CO2 + hv | - |
? | |
1.13.12.13 | Oplophorus luciferin + O2 | Oplophorus gracilirostris | - |
oxidized Oplophorus luciferin + CO2 + hv | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.13.12.13 | Oplophorus gracilirostris | Q9GV45 AND Q9GV46 | catalytic and noncatalytic subunit | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.13.12.13 | recombinant His-tagged catalytic subunit mutant nanoKAZ from Escherichia coli strain BL21 by nickel affinity chromatography and dialysis, wild-typeKAZ is purified from inclusion bodies using 6 M urea-nickel chelate affinity column chromatography | Oplophorus gracilirostris |
EC Number | Renatured (Comment) | Organism |
---|---|---|
1.13.12.13 | wild-typeKAZ is purified from inclusion bodies using 6 M urea-nickel chelate affinity column chromatography | Oplophorus gracilirostris |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.13.12.13 | coelenterazine + O2 | - |
Oplophorus gracilirostris | coelenteramide + CO2 + hv | - |
? | |
1.13.12.13 | coelenterazine + O2 | a luciferin substrate | Oplophorus gracilirostris | coelenteramide + CO2 + hv | - |
? | |
1.13.12.13 | Oplophorus luciferin + O2 | - |
Oplophorus gracilirostris | oxidized Oplophorus luciferin + CO2 + hv | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.13.12.13 | heterooligomer | x * 19000, catalytic subunit, + x * 35000, noncatalytic subunit, SDS-PAGE | Oplophorus gracilirostris |
1.13.12.13 | More | the structure of the mutant catalytic subunit nanoKAZ consists of 11 antiparallel beta-strands forming a beta-barrel that is capped by 4 short alpha-helices. The coelenterazine-binding site and the catalytic site for the luminescence reaction might be in a central cavity of the beta-barrel structure of the catalytic subunit KAZ | Oplophorus gracilirostris |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.13.12.13 | KAZ | catalytic enzyme subunit | Oplophorus gracilirostris |
1.13.12.13 | Oplophorus luciferase | - |
Oplophorus gracilirostris |
EC Number | General Information | Comment | Organism |
---|---|---|---|
1.13.12.13 | evolution | the enzyme is a member of the coelenterazine-utilizing luciferases such as Renilla luciferase and Gaussia luciferase | Oplophorus gracilirostris |
1.13.12.13 | additional information | the coelenterazine-binding site and the catalytic site for the luminescence reaction might be in a central cavity of the beta-barrel structure of the catalytic subunit KAZ | Oplophorus gracilirostris |